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CLC number: R730.1; R730.231.3

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Received: 2005-01-18

Revision Accepted: 2005-01-30

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.4 P.236~241

http://doi.org/10.1631/jzus.2005.B0236


Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression


Author(s):  CHEN Zhe, XU Ze-feng, YE Jing-jia, YAO Hang-ping, ZHENG Shu, DING Jia-yi

Affiliation(s):  Cancer Institute, Second affiliated hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   Zhengshu@mail.hz.zj.cn, djy@zju.edu.cn

Key Words:  Hepatitis B virus, Combination of siRNAs, HBV replication, Antigen expression


CHEN Zhe, XU Ze-feng, YE Jing-jia, YAO Hang-ping, ZHENG Shu, DING Jia-yi. Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression[J]. Journal of Zhejiang University Science B, 2005, 6(4): 236~241.

@article{title="Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression",
author="CHEN Zhe, XU Ze-feng, YE Jing-jia, YAO Hang-ping, ZHENG Shu, DING Jia-yi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="4",
pages="236~241",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0236"
}

%0 Journal Article
%T Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression
%A CHEN Zhe
%A XU Ze-feng
%A YE Jing-jia
%A YAO Hang-ping
%A ZHENG Shu
%A DING Jia-yi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 4
%P 236~241
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0236

TY - JOUR
T1 - Combination of small interfering RNAs mediates greater inhibition of human hepatitis B virus replication and antigen expression
A1 - CHEN Zhe
A1 - XU Ze-feng
A1 - YE Jing-jia
A1 - YAO Hang-ping
A1 - ZHENG Shu
A1 - DING Jia-yi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 4
SP - 236
EP - 241
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0236


Abstract: 
Objectives: To evaluate the inhibitory effect mediated by combination of small interfering RNAs (siRNAs) targeting different sites of hepatitis B virus (HBV) transcripts on the viral replication and antigen expression in vitro. Methods: (1) Seven siRNAs targeting surface (S), polymerase (P) or precore (PreC) region of HBV genome were designed and chemically synthesized. (2) HBV-producing HepG2.2.15 cells were treated with or without siRNAs for 72 h. (3) HBsAg and HBeAg in the cell culture medium were detected by enzyme-linked immunoadsorbent assay. (4) Intracellular viral DNA was quantified by real-time PCR (Polymerase Chain Reaction). (5) HBV viral mRNA was reverse transcribed and quantified by real-time PCR. (6) The change of cell cycle and apoptosis was determined by flow cytometry. Results: Our data demonstrated that synthetic small interfering RNAs (siRNAs) targeting S and PreC gene could efficiently and specifically inhibit HBV replication and antigen expression. The expression of HBsAg and HBeAg and the replication of HBV could be specifically inhibited in a dose-dependent manner by siRNAs. Furthermore, our results showed that the combination of siRNAs targeting various regions could inhibit HBV replication and antigen expression in a more efficient way than the use of single siRNA at the same final concentration. No apoptotic change was observed in the cell after siRNA treatment. Conclusion: Our results demonstrated that siRNAs exerted robust and specific inhibition on HBV replication and antigen expression in a cell culture system and combination of siRNAs targeting different regions exhibited more potency.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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