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CLC number: Q27

On-line Access: 2010-11-04

Received: 2010-05-19

Revision Accepted: 2010-08-01

Crosschecked: 2010-10-13

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.11 P.889-894

10.1631/jzus.B1000184


Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells


Author(s):  Shao-zhi Zhang, Huan Qian, Zhen Wang, Ju-li Fan, Qian Zhou, Guang-ming Chen, Rui Li, Shan Fu, Jie Sun

Affiliation(s):  Refrigeration and Cryogenic Engineering Institute, Zhejiang University, Hangzhou 310027, China, Bone Marrow Transplantation Center, the First Affiliate Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   jsun1492@gmail.com

Key Words:  Human bone marrow-derived mesenchymal stem cells (hBM-MSCs), Freeze-drying, Trehalose, Rehydration


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Shao-zhi Zhang, Huan Qian, Zhen Wang, Ju-li Fan, Qian Zhou, Guang-ming Chen, Rui Li, Shan Fu, Jie Sun. Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells[J]. Journal of Zhejiang University Science B, 2010, 11(11): 889-894.

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author="Shao-zhi Zhang, Huan Qian, Zhen Wang, Ju-li Fan, Qian Zhou, Guang-ming Chen, Rui Li, Shan Fu, Jie Sun",
journal="Journal of Zhejiang University Science B",
volume="11",
number="11",
pages="889-894",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000184"
}

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%T Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells
%A Shao-zhi Zhang
%A Huan Qian
%A Zhen Wang
%A Ju-li Fan
%A Qian Zhou
%A Guang-ming Chen
%A Rui Li
%A Shan Fu
%A Jie Sun
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 11
%P 889-894
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000184

TY - JOUR
T1 - Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells
A1 - Shao-zhi Zhang
A1 - Huan Qian
A1 - Zhen Wang
A1 - Ju-li Fan
A1 - Qian Zhou
A1 - Guang-ming Chen
A1 - Rui Li
A1 - Shan Fu
A1 - Jie Sun
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 11
SP - 889
EP - 894
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000184


Abstract: 
Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33±13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Alpert, P., 2006. Constraints of tolerance: why are desiccation-tolerant organisms so small or rare? J. Exp. Biol., 209(9):1575-1584.

[2]Carvalho, K.A., Cury, C.C., Oliveira, L., Cattaned, R.I., Malvezzi, M., Francisco, J.C., Pachalok, A., Olandoski, M., Faria-Neto, J.R., Guarita-Souza, L.C., 2008. Evaluation of bone marrow mesenchymal stem cell standard cryopreservation procedure efficiency. Transp. Proc., 40(3):839-841.

[3]Chen, T., Acker, J.P., Eroglu, A., 2001. Beneficial effect of intracellular trehalose on the membrane integrity of dried mammalian cells. Cryobiology, 43(2):168-181.

[4]Crowe, J.H., Crowe, L.M., Oliver, A.E., 2001. The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state. Cryobiology, 43(2):89-105.

[5]Eroglu, A., Toner, M., Toth, T.L., 2002. Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes. Fertil. Steril., 77(1):152-158.

[6]Gordon, S.L., Oppenheimer, S.R., Mackay, A.M., 2001. Recovery of human mesenchymal stem cells following dehydration and rehydration. Cryobiology, 43(2):182-187.

[7]Han, Y., Quan, G.B., Liu, X.Z., Liu, A., Jin, P., Cao, W., 2004. Effect of reconstitution solutions on the recovery of lyophilized red blood cells. Chin. J. Blood Transfus., 17(1):308-311.

[8]Han, Y., Quan, G.B., Liu, X.Z., 2005. Improved preservation of human red blood cells by lyophilization. Cryobiology, 51(2):152-164.

[9]Jamil, K., Crowe, J.H., Tablin, F., Oliver, A.E., 2005. Arbutin enhances recovery and osteogenic differentiation in dried and rehydrated human mesenchymal stem cells. Cell Preserv. Technol., 3(4):244-255.

[10]Li, J., Hua, T.C., Gu, X.L., 2005. Morphology study of freeze-drying mononuclear cells of human cord blood. CryoLetters, 26(3):193-200.

[11]Li, X.S., Fan, H.T., Yuan, Y., Hou, C.M., Mao, N., 2003. Modification of in situ cryopreservation of human bone marrow mesenchymal stem cells. J. Exp. Hematol., 11(5):530-533 (in Chinese).

[12]Luo, Y., Huang, H., Yu, J., Liang, B., 2006. Effect of cryopreservation on the biological characteristics of adult mesenchymal stem cells derived from bone marrow. Chin. J. Pathophysiol., 2(2):384-387 (in Chinese).

[13]Nakamizo, F., Marini, T., Amano, T., 2005. Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas. Cancer Res., 64(4):3307-3318.

[14]Oliver, A., Jamil, K., Crowe, J.H., Tablin, F., 2004. Loading human mesenchymal stem cells with trehalose by fluid-phase endocytosis. Cell Preserv. Technol., 2(1):35-49.

[15]Potts, M., Slaughter, S.M., Hunneke, F.U., 2005. Desiccation tolerance of prokaryotes: application of principles to human cells. Integr. Comp. Biol., 45(5):800-809.

[16]Shirakashi, R., Kostner, C.M., Muller, K.J., 2002. Intracellular delivery of trehalose into mammalian cells by electropermeabilization. J. Membr. Boil., 189(1):45-54.

[17]Török, Z., Satpathy, G.R., Banerjee, M., Bali, R., Little, E., Novaes, R., Ly, H.V., Dwyre, D.M., Kheirolomoom, A., Tablin, F., et al., 2005. Preservation of trehalose-loaded red blood cells by lyophilization. Cell Preserv. Technol., 3(2):96-111.

[18]Umbreit, W.W., Burris, R.H., Stauffer, J.F., 1972. Manometric and Biochemical Techniques, 5th Ed. Burgess Publishing Company, Minneapolis, USA, p.262.

[19]Wang, J., Zhang, H., 2007. The influence of one-step −80 °C cryopreservation on the osteogenesis differentiation ability of human bone marrow-derived mesenchymal stem cells. Guangdong Med., 28(3):365-367 (in Chinese).

[20]Weng, Y., Zhou, X.L., Chen, G.M., 2004. The development of a freeze-dryer. Low Temp. Technol., 44(3):12-16 (in Chinese).

[21]Winer, J.P., Janmey, P.A., McCormick, M.E., Funaki, M., 2009. Bone marrow-derived human mesenchymal stem cells become quiescent on soft substrates but remain responsive to chemical or mechanical stimuli. Tissue Eng. Part A, 15(1):147-154.

[22]Wolkers, W.F., Walker, N.J., Tablin, F., Crowe, J.H., 2001. Human platelets loaded with trehalose survive freeze-drying. Cryobiology, 42(2):79-87.

[23]Wolkers, W.F., Walker, N.J., Tamari, Y., 2002. Towards a clinical application of freeze-dried human platelets. Cell Preserv. Technol., 1(3):175-188.

[24]Xiang, Y., Zheng, Q., Jia, B., Huang, G., Xie, C., Pan, J., Wang, J., 2007. Ex vivo expansion, adipogenesis and neurogenesis of cryopreserved human bone marrow mesenchymal stem cells. J. Zhejiang Univ.-Sci. B, 8(2):136-146.

[25]Xiao, H.H., Hua, T.C., Li, J., 2004. Freeze drying of mononuclear cells and whole blood of human cord blood. CryoLetters, 25(2):111-120.

[26]Yang, F., Cheng, Q.K., Wang, X., Hua, Z.Z., Chen, F.F., Shu, C.F., Cui, L., Cao, Y.L., 2005. Research on freeze-drying of human bone mesenchymal stem cells. J. Refriger., 26(1):19-23 (in Chinese).

[27]Zeng, X.M., Martin, G.P., Marriott, C., 2001. Effects of molecular weight of polyvinylpyrrolidone on the glass transition and crystallization of co-lyophilized sucrose. Int. J. Pharm., 218(1-2):63-73.

[28]Zhang, S.Z., Fan, J.L., Xu, X.G., 2009. An experimental study of the use of ultrasound to facilitate the loading of trehalose into platelets. Cryobiology, 59(2):135-140.

[29]Zhou, X.L., Zhu, H., Zhang, S.Z., Zhu, F.M., Chen, G.M., Yan, L.X., 2007. Freeze-drying of human platelets: influence of saccharide, freezing rate and cell concentration. CryoLetters, 28(3):187-196.

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