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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.4 P.248~255


Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation

Author(s):  WANG Guan-yu, ZHANG Jia-wei, LÜ, Qing-hua, XU Rong-zhen, DONG Qing-hua

Affiliation(s):  Department of Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more

Corresponding email(s):   wangguanyu@zju.edu.cn, dongqinghua@zju.edu.cn

Key Words:  Berbamine, Apoptosis, Mitochondrial membrane potential, Caspase, Hepatoma

WANG Guan-yu, ZHANG Jia-wei, LÜ Qing-hua, XU Rong-zhen, DONG Qing-hua. Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation[J]. Journal of Zhejiang University Science B, 2007, 8(4): 248~255.

@article{title="Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation",
author="WANG Guan-yu, ZHANG Jia-wei, LÜ Qing-hua, XU Rong-zhen, DONG Qing-hua",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation
%A WANG Guan-yu
%A ZHANG Jia-wei
%A Qing-hua
%A XU Rong-zhen
%A DONG Qing-hua
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 4
%P 248~255
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0248

T1 - Berbamine induces apoptosis in human hepatoma cell line SMMC7721 by loss in mitochondrial transmembrane potential and caspase activation
A1 - WANG Guan-yu
A1 - ZHANG Jia-wei
A1 - LÜ
A1 - Qing-hua
A1 - XU Rong-zhen
A1 - DONG Qing-hua
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 4
SP - 248
EP - 255
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0248

Objective: To investigate the effect of berbamine on human hepatoma cell line SMMC7721. Methods: The effects of 24 h and 48 h incubation with different concentrations (0~64 μg/ml) of the berbamine on SMMC7721 cells were evaluated using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Hoechst 33258 staining was conducted to distinguish the apoptotic cell, and the appearance of sub-G1 stage was determined by PI (propidium iodide) staining, the percentage of apoptotic cell was determined by flow cytometry following annexin V/PI staining. Flow cytometry was performed to analyze the cell cycle distribution and the mitochondrial membrane potential (∆ψm); the expression of activated caspase3 and caspase9 was analyzed by Western-blot. Results: The proliferation of SMMC7721 was decreased after treatment with berbamine in a dose- and time-dependent manner. berbamine could induce apoptosis in SMMC7721 cells and could cause cell cycle arrest in G0/G1 phase, to induce loss of mitochondrial membrane potential (∆ψm) and activate caspase3 and caspase9. berbamine-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk. Conclusion: berbamine exerts antiproliferative effects on human hepatocellular carcinoma SMMC7721 cells. The anticancer activity of berbamine could be attributed partly to its inhibition of cell proliferation and induction of apoptosis in cancer cells through loss in mitochondrial transmembrane potential and caspase activation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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