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On-line Access: 2010-12-09

Received: 2010-04-20

Revision Accepted: 2010-07-22

Crosschecked: 2010-11-05

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.12 P.965-972

10.1631/jzus.B1000147


Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination


Author(s):  Yu-qin Mei, Song-quan Song

Affiliation(s):  Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Corresponding email(s):   sqsong@ibcas.ac.cn

Key Words:  Barley seed, Cross-tolerance, Germination/growth, Reactive oxygen species (ROS) scavenging enzyme, Pretreatment at low temperature, Temperature stress


Yu-qin Mei, Song-quan Song. Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination[J]. Journal of Zhejiang University Science B, 2010, 11(12): 965-972.

@article{title="Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination",
author="Yu-qin Mei, Song-quan Song",
journal="Journal of Zhejiang University Science B",
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year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000147"
}

%0 Journal Article
%T Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination
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%A Song-quan Song
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 12
%P 965-972
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000147

TY - JOUR
T1 - Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination
A1 - Yu-qin Mei
A1 - Song-quan Song
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 12
SP - 965
EP - 972
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000147


Abstract: 
A number of studies have shown the existence of cross-tolerance in plants, but the physiological mechanism is poorly understood. In this study, we used the germination of barley seeds as a system to investigate the cross-tolerance of low-temperature pretreatment to high-temperature stress and the possible involvement of reactive oxygen species (ROS) scavenging enzymes in the cross-tolerance. After pretreatment at 0 °C for different periods of time, barley seeds were germinated at 35 °C, and the content of malondialdehyde (MDA) and the activities of ROS scavenging enzymes were measured by a spectrophotometer analysis. The results showed that barley seed germinated very poorly at 35 °C, and this inhibitive effect could be overcome by pretreatment at 0 °C. The MDA content varied, depending on the temperature at which seeds germinated, while barley seeds pretreated at 0 °C did not change the MDA content. Compared with seeds germinated directly at 35 °C, the seeds pretreated first at 0 °C and then germinated at 35 °C had markedly increased activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR). The SOD and APX activities of seeds germinated at 35 °C after 0 °C-pretreatment were even substantially higher than those at 25 °C, and GR activity was similar to that at 25 °C, at which the highest germination performance of barley seeds was achieved. These results indicate that low-temperature pretreatment can markedly increase the tolerance of barley seed to high temperature during germination, this being related to the increase in ROS scavenging enzyme activity. This may provide a new method for increasing seed germination under stress environments, and may be an excellent model system for the study of cross-tolerance.

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