Full Text:   <1187>

Summary:  <375>

CLC number: S828

On-line Access: 2016-10-02

Received: 2015-08-13

Revision Accepted: 2016-02-17

Crosschecked: 2016-09-11

Cited: 3

Clicked: 1671

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hong-nan Liu

http://orcid.org/0000-0002-5710-8624

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.10 P.752-762

http://doi.org/10.1631/jzus.B1500192


Effects of yeast products on the intestinal morphology, barrier function, cytokine expression, and antioxidant system of weaned piglets


Author(s):  Huan-sheng Yang, Fei Wu, Li-na Long, Tie-jun Li, Xia Xiong, Peng Liao, Hong-nan Liu, Yu-long Yin

Affiliation(s):  Observation and Experiment Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture; more

Corresponding email(s):   xx@isa.ac.cn, liuhn@isa.ac.cn

Key Words:  Yeast products, Weaned piglet, Intestinal morphology, Barrier function, Antioxidant system


Huan-sheng Yang, Fei Wu, Li-na Long, Tie-jun Li, Xia Xiong, Peng Liao, Hong-nan Liu, Yu-long Yin. Effects of yeast products on the intestinal morphology, barrier function, cytokine expression, and antioxidant system of weaned piglets[J]. Journal of Zhejiang University Science B, 2016, 17(10): 752-762.

@article{title="Effects of yeast products on the intestinal morphology, barrier function, cytokine expression, and antioxidant system of weaned piglets",
author="Huan-sheng Yang, Fei Wu, Li-na Long, Tie-jun Li, Xia Xiong, Peng Liao, Hong-nan Liu, Yu-long Yin",
journal="Journal of Zhejiang University Science B",
volume="17",
number="10",
pages="752-762",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500192"
}

%0 Journal Article
%T Effects of yeast products on the intestinal morphology, barrier function, cytokine expression, and antioxidant system of weaned piglets
%A Huan-sheng Yang
%A Fei Wu
%A Li-na Long
%A Tie-jun Li
%A Xia Xiong
%A Peng Liao
%A Hong-nan Liu
%A Yu-long Yin
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 10
%P 752-762
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500192

TY - JOUR
T1 - Effects of yeast products on the intestinal morphology, barrier function, cytokine expression, and antioxidant system of weaned piglets
A1 - Huan-sheng Yang
A1 - Fei Wu
A1 - Li-na Long
A1 - Tie-jun Li
A1 - Xia Xiong
A1 - Peng Liao
A1 - Hong-nan Liu
A1 - Yu-long Yin
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 10
SP - 752
EP - 762
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500192


Abstract: 
The goal of this study was to evaluate the effects of a mixture of yeast culture, cell wall hydrolysates, and yeast extracts (collectively “yeast products,” YP) on the performance, intestinal physiology, and health of weaned piglets. A total of 90 piglets weaned at 21 d of age were blocked by body weight, sex, and litter and randomly assigned to one of three treatments for a 14-d feeding experiment, including (1) a basal diet (control), (2) 1.2 g/kg of YP, and (3) 20 mg/kg of colistin sulfate (CSE). No statistically significant differences were observed in average daily feed intake, average daily weight gain, or gain-to-feed ratio among CSE, YP, and control piglets. Increased prevalence of diarrhea was observed among piglets fed the YP diet, whereas diarrhea was less prevalent among those fed CSE. Duodenal and jejunal villus height and duodenal crypt depth were greater in the control group than they were in the YP or CSE groups. Intraepithelial lymphocytes (IEL) in the duodenal and jejunal villi were enhanced by YP, whereas IEL in the ileal villi were reduced in weaned piglets fed YP. Secretion of jejunal and ileal interleukin-10 (IL-10) was higher and intestinal and serum antioxidant indexes were affected by YP and CSE. In YP- and CSE-supplemented animals, serum D-lactate concentration and diamine oxidase (DAO) activity were both increased, and intestinal mRNA expressions of occludin and ZO-1 were reduced as compared to the control animals. In conclusion, YP supplementation in the diets of weaned piglets appears to increase the incidence of diarrhea and has adverse effects on intestinal morphology and barrier function.

酵母产物对断奶仔猪肠道形态、屏障功能、细胞因子表达和抗氧化系统的影响

目的:验证添加酵母混合物(酵母培养物、酵母细胞壁水解物和酵母提取物)对断奶仔猪生长性能、腹泻发生率、肠道形态、屏障功能、免疫反应和抗氧化系统的影响。
创新点:考察酵母培养物、酵母细胞壁水解物和酵母提取物混合物对断奶仔猪的协同作用。
方法:90头21日龄断奶仔猪随机分为3组,分别饲喂基础日粮(对照组),含1.2 g/kg的酵母混合物(YP组)及含20 mg/kg硫酸粘杆菌素日粮(CSE组)14天,比较三组间各项指标差异。
结论:结果表明,三组之间平均日采食量、平均日增重和料肉比无显著差异。YP组腹泻发生率显著高于其它两组。对照组十二指肠和空肠的绒毛高度以及十二指肠的隐窝深度显著高于YP组和CSE组。相对于对照组或CSE组,YP组十二指肠和空肠绒毛淋巴细胞数目显著增加,而回肠绒毛内淋巴细胞数目显著降低。相对于对照组,YP组空肠和回肠内白介素-10(IL-10)的分泌增加,YP和CSE也显著影响了仔猪肠道和血清中抗氧化因子;YP和CSE组血清d-乳酸浓度和二胺氧化酶活性都增强;YP组或CSE组肠道occludinZO-1 mRNA表达降低。综上所述,酵母混合物添加会增加断奶仔猪腹泻发生率,并对断奶仔猪肠道形态学和屏障功能具有副作用。

关键词:酵母产物;断奶仔猪;肠道形态;屏障功能;抗氧化系统

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Blecha, F., Charley, B., 1990. Rationale for using immunopotentiators in domestic food animals. Adv. Vet. Sci. Comp. Med., 35:3-19.

[2]Boudry, G., Péron, V., le Huërou-Luron, I., et al., 2004. Weaning induces both transient and long-lasting modifications of absorptive, secretory, and barrier properties of piglet intestine. J. Nutr., 134:2256-2262.

[3]Carlson, M.S., Veum, T.L., Turk, J.R., 2005. Effects of yeast extract versus animal plasma in weanling pig diets on growth performance and intestinal morphology. J. Swine Health Prod., 13:204-209.

[4]Clark, I.A., 2007. How TNF was recognized as a key mechanism of disease. Cytokine Growth Factor Rev., 18(3-4):335-343.

[5]Fleck, C.C., Carey, H.V., 2005. Modulation of apoptotic pathways in intestinal mucosa during hibernation. AJP Regul. Integr. Comp. Physiol., 289(2):R586-R595.

[6]Granot, E., Kohen, R., 2004. Oxidative stress in childhood—in health and disease states. Clin. Nutr., 23(1):3-11.

[7]Hahn, T.W., Lohakare, J.D., Lee, S.L., et al., 2006. Effects of supplementation of β-glucans on growth performance, nutrient digestibility, and immunity in weanling pigs. J. Anim. Sci., 84(6):1422-1428.

[8]He, Q.H., Tang, H.R., Ren, P.P., et al., 2011. Dietary supplementation with L-arginine partially counteracts serum metabonome induced by weaning stress in piglets. J. Proteome Res., 10(11):5214-5221.

[9]Irshad, M., Chaudhuri, P.S., 2002. Oxidant-antioxidant system: role and significance in human body. Indian J. Exp. Biol., 40(11):1233-1239.

[10]Jiang, R., Chang, X., Stoll, B., et al., 2000. Dietary plasma protein reduces small intestinal growth and lamina propria cell density in early weaned pigs. J. Nutr., 130(1):21-26.

[11]Jiang, Z.Y., Sun, L.H., Lin, Y.C., et al., 2009. Effects of dietary glycyl-glutamine on growth performance, small intestinal integrity, and immune responses of weaning piglets challenged with lipopolysaccharide. J. Anim. Sci., 87(12):4050-4056.

[12]Kang, P., Wang, M., Hou, Y.Q., et al., 2012. Effects of oral administration of spermine on the development of small intestine and growth performance of weaned pigs. J. Anim. Vet. Adv., 11(15):2782-2787.

[13]Kaplan, M., Mutlu, E.A., Benson, M., et al., 2007. Use of herbal preparations in the treatment of oxidant-mediated inflammatory disorders. Complement. Ther. Med., 15(3):207-216.

[14]King, M.R., Kelly, D., Morel, P.C.H., et al., 2003. Aspects of intestinal immunity in the pig around weaning. In: Pluske, J.R., Dividich, J.L., Verstegen, M.W.A. (Eds.), Weaning the Pig: Concepts and Consequences. Wageningen Academic Publishers, the Netherlands, p.219-257.

[15]Kogan, G., Kocher, A., 2007. Role of yeast cell wall polysaccharides in pig nutrition and health protection. Livest. Sci., 109(1-3):161-165.

[16]Kogan, G., Staško, A., Bauerová, K., et al., 2005. Antioxidant properties of yeast (1→3)-β-D-glucan studied by electron paramagnetic resonance spectroscopy and its activity in the adjuvant arthritis. Carbohyd. Polym., 61(1):18-28.

[17]Liu, P., Piao, X.S., Kim, S.W., et al., 2008. Effects of chito-oligosaccharide supplementation on the growth performance, nutrient digestibility, intestinal morphology, and fecal shedding of Escherichia coli and Lactobacillus in weaning pigs. J. Anim. Sci., 86(10):2609-2618.

[18]Luk, G.D., Bayless, T.M., Baylin, S.B., 1980. Diamine oxidase (histaminase): a circulating marker for rat intestinal mucosal maturation and integrity. J. Clin. Invest., 66(1):66-70.

[19]Maribo, H., Spring, P., 2003. Yeast extract as a protein source for weaning piglets. In: Schubert, R., Flachowsky, G., Jahreis, G., et al. (Eds.), Vitamine und Zusatzstoffe in der Ernährung von Mensch und Tier. Jena Thüringen, p.433-437.

[20]Montagne, L., Boudry, G., Favier, C., et al., 2007. Main intestinal markers associated with the changes in gut architecture and function in piglets after weaning. Brit. J. Nutr., 97(1):45-57.

[21]NRC (National Research Council), 2012. Nutrient Requirements of Swine: eleventh revised Ed. National Academic Press, Washington, DC.

[22]Pié, S., Lallès, J.P., Blazy, F., et al., 2004. Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets. J. Nutr., 134(3):641-647.

[23]Pluske, J.R., Williams, I.H., Aherne, F.X., 1996. Nutrition of the neonatal pig. In: Varley, M.A. (Ed.), The Neonatal Pig: Development and Survival. CAB Int., Wallingford, p.187-235.

[24]Pourahmad, J., Shaki, F., Tanbakosazan, F., et al., 2011. Protective effects of fungal β-(1→3)-D-glucan against oxidative stress cytotoxicity induced by depleted uranium in isolated rat hepatocytes. Human Exp. Toxicol., 30(3):173-181.

[25]Ren, W.K., Yin, Y.L., Liu, G., et al., 2012. Effect of dietary arginine supplementation on reproductive performance of mice with porcine circovirus type 2 (PCV2) infection. Amino Acids, 42(6):2089-2094.

[26]Schiepers, O.J., Wichers, M.C., Maes, M., 2005. Cytokines and major depression. Prog. Neuro-Psychopharmacol. Biol. Psychiatry, 29(2):201-217.

[27]Shen, Y.B., Piao, X.S., Kim, S.W., et al., 2009. Effects of yeast culture supplementation on growth performance, intestinal health, and immune response of nursery pigs. J. Anim. Sci., 87(8):2614-2624.

[28]Sun, Z.W., Wang, X.D., Andersson, R., 1998. Role of intestinal permeability in monitoring mucosal barrier function. Digest. Surg., 15(5):386-397.

[29]Tan, B.E., Yin, Y.L., Liu, Z.Q., et al., 2009. Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids, 37(1):169-175.

[30]Tan, B.E., Yin, Y.L., Liu, Z.Q., et al., 2011. Dietary L-arginine supplementation differentially regulates expression of fat-metabolic genes in porcine adipose tissue and skeletal muscle. J. Nutr. Biochem., 22(5):441-445.

[31]Ushida, K., Kameue, C., Tsukahara, T., et al., 2008. Decreasing traits of fecal immunoglobulin A in neonatal and weaning piglets. J. Vet. Med. Sci., 70(8):849-852.

[32]van den Bogaard, A.E., Stobberingh, E.E., 2000. Epidemiology of resistance to antibiotics: links between animals and humans. Int. J. Antimicrob. Agents, 14(4):327-335.

[33]van der Peet-Schwering, C.M., Jansman, A.J., Smidt, H., et al., 2007. Effects of yeast culture on performance, gut integrity, and blood cell composition of weanling pigs. J. Anim. Sci., 85(11):3099-3109.

[34]van Heugten, E., Funderburke, D.W., Dorton, K.L., 2003. Growth performance, nutrient digestibility, and fecal microflora in weanling pigs fed live yeast. J. Anim. Sci., 81(4):1004-1012.

[35]Vella, A., Farrugia, G., 1998. D-Lactic acidosis: pathologic consequence of saprophytism. Mayo Clin. Proc., 73(5):451-456.

[36]Verdonk, J.M., Bruininx, E.M., van der Meulen, J., et al., 2007. Post-weaning feed intake level modulates gut morphology but not gut permeability in weaned piglets. Livest. Sci., 108(1-3):146-149.

[37]Wang, J., Chen, L., Li, P., et al., 2008. Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J. Nutr., 138(11):1025-1032.

[38]Wu, X., Yin, Y.L., Li, T.J., et al., 2010. Dietary supplementation with L-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in weanling pigs fed a corn- and soybean meal-based diet. Amino Acids, 39(3):831-839.

[39]Wu, X., Zhang, Y., Liu, Z., et al., 2015. Effects of oral supplementation with glutamate or combination of glutamate and N-carbamylglutamate on intestinal mucosa in piglets. J. Anim. Sci., 90(Suppl. 4):337-339.

[40]Yang, H., Fu, D., Shao, H., et al., 2012. Impacts of birth weight on plasma, liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets. PLOS ONE, 7:e50921.

[41]Yang, H., Li, F., Xiong, X., et al., 2013. Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigs. Mol. Cell. Endocrinol., 365(1):44-51.

[42]Yin, F.G., Zhang, Z.Z., Huang, J., et al., 2010. Digestion rate of dietary starch affects systemic circulation of amino acids in weaned pigs. Brit. J. Nutr., 103(10):1404-1412.

[43]Yin, Y.L., Yao, K., Liu, Z.J., et al., 2010. Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs. Amino Acids, 39(5):1477-1486.

[44]Yin, J., Ren, W.K., Liu, G., et al., 2013. Birth oxidative stress and the development of an antioxidant system in newborn piglets. Free Radical Res., 47(12):1027-1035.

[45]Zelnickova, P., Leva, L., Stepanova, H., et al., 2008. Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes. Vet. Immunol. Immunopathol., 124(3-4):367-378.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE