Full Text:   <554>

Summary:  <166>

CLC number: 

On-line Access: 2023-11-14

Received: 2022-12-14

Revision Accepted: 2023-04-16

Crosschecked: 2023-11-15

Cited: 0

Clicked: 657

Citations:  Bibtex RefMan EndNote GB/T7714


Xianghua YAN


-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.11 P.1014-1026


Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens

Author(s):  Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN

Affiliation(s):  National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology, Huazhong Agricultural University,Wuhan430070,China; more

Corresponding email(s):   xhyan@mail.hzau.edu.cn

Key Words:  Chicken, Aeriscardovia aeriphila, Gut health, Microbiome, Immune function

Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN. Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens[J]. Journal of Zhejiang University Science B, 2023, 24(11): 1014-1026.

@article{title="Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens",
author="Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens
%A Muhammad Zahid FAROOQ
%A Xinkai WANG
%A Xianghua YAN
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 11
%P 1014-1026
%@ 1673-1581
%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200621

T1 - Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens
A1 - Muhammad Zahid FAROOQ
A1 - Xinkai WANG
A1 - Xianghua YAN
J0 - Journal of Zhejiang University Science B
VL - 24
IS - 11
SP - 1014
EP - 1026
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2200621

Aeriscardovia aeriphila, also known asBifidobacterium aerophilum, was first isolated from the caecal contents of pigs and the faeces of cotton-top tamarin.Bifidobacterium species play important roles in preventing intestinal infections, decreasing cholesterol levels, and stimulating the immune system. In this study, we isolated a strain of bacteria from the duodenal contents of broiler chickens, which was identified asA. aeriphila, and then evaluated the effects ofA. aeriphila on growth performance, antioxidant functions, immune functions, and gut microbiota in commercial broiler chickens. chickens were orally gavaged withA. aeriphila (1×109 CFU/mL) for 21 d. The results showed thatA. aeriphila treatment significantly increased the average daily gain and reduced the feed conversion ratio (P<0.001). The levels of serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) were significantly increased followingA. aeriphila treatment (P<0.05). Blood urea nitrogen and aspartate aminotransferase levels were decreased, whereas glucose and creatinine levels increased as a result ofA. aeriphila treatment. Furthermore, the levels of serum antioxidant enzymes, including catalase (P<0.01), superoxide dismutase (P<0.001), and glutathione peroxidase (P<0.05), and total antioxidant capacity (P<0.05) were enhanced followingA. aeriphila treatment.A. aeriphila treatment significantly increased the levels of serum immunoglobulin A (IgA) (P<0.05), IgG (P<0.01), IgM (P<0.05), interleukin-1 (IL-1) (P<0.05), IL-4 (P<0.05), and IL-10 (P<0.05). The broiler chickens in theA. aeriphila group had higher secretory IgA (SIgA) levels in the duodenum (P<0.01), jejunum (P<0.001), and cecum (P<0.001) than those in the control group. The messenger RNA (mRNA) relative expression levels ofIL-10 (P<0.05) andIL-4 (P<0.001) in the intestinal mucosa of chickens were increased, while nuclear factor-‍κB (NF-κB) (P<0.001) expression was decreased in theA. aeriphila group compared to the control group. Phylum-level analysis revealed Firmicutes as the main phylum, followed by Bacteroidetes, in both groups. The data also found thatPhascolarctobacterium andBarnesiella were increased inA. aeriphila-treated group. In conclusion, oral administration ofA. aeriphila could improve the growth performance, serum antioxidant capacity, immune modulation, and gut health of broilers. Our findings may provide important information for the application ofA. aeriphila in poultry production.

Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响

Muhammad Zahid FAROOQ1,2,王新锴1,晏向华1
摘要:Aeriscardovia aeriphila,又名Bifidobacterium aerophilum,是从猪盲肠内容物中分离出的一种双歧杆菌。A. aeriphila在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌,经16S 测序鉴定为 A. aeriphila,然后我们评估了A. aeriphila对商用肉鸡生长性能、抗氧化功能、免疫功能和肠道菌群的影响。实验对21日龄的实验鸡进行了为期3周的灌服A. aeriphila(1×109 CFU/mL)处理。研究结果显示:A. aeriphila处理显著增加了鸡的平均日增重,降低了饲料转化率(P<0.001)。A. aeriphila处理后,血清生长激素(GH)和胰岛素样生长因子1(IGF-1)水平显著升高(P<0.05)。A. aeriphila处理降低了血尿素氮和天门冬氨酸氨基转移酶水平,增加了血糖和肌酐水平。同时,A. aeriphila处理显著提高了血清中抗氧化酶水平(包括过氧化氢酶(P<0.01)、超氧化物歧化酶(P<0.001)和谷胱甘肽过氧化物酶(P<0.05))及总抗氧化能力(P<0.05)。A. aeriphila处理显著增加了血清免疫球蛋白A(IgA)(P<0.05)、IgG(P<0.01)、IgM(P<0.05)、白细胞介素-1(IL-1)(P<0.05)、IL-4(P<0.05)和IL-10(P<0.05)的水平。与对照组相比,A. aeriphila组肉鸡在十二指肠(P<0.01)、空肠(P<0.001)和盲肠(P<0.001)中的分泌型免疫球蛋白A(SIgA)水平显著提高。在肠道黏膜(包括十二指肠、空肠和盲肠)中,A. aeriphila组鸡的IL-10P<0.05)、IL-4P<0.001)和NF-κBP<0.001)信使RNA(mRNA)相对表达水平高于对照组。肠道微生物门水平分析显示,两组的差异菌群主要是厚壁菌门,其次是拟杆菌门。此外,A. aeriphila可提高肠道中PhascolarctobacteriumBarnesiella的丰度。综上所述,灌服A. aeriphila可改善肉鸡的生长性能、血清抗氧化能力、免疫调节和肠道健康。因此,本研究结果可以为A. aeriphila在家禽生产中的应用提供理论依据。

关键词:鸡;Aeriscardovia aeriphila;肠道健康;菌群;免疫功能

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]AbdulrahimSM,HaddadinMSY,HashlamounEAR,et al.,1996.The influence ofLactobacillusacidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk.Br Poultry Sci,37(2):341-346.

[2]AdalsteinsdottirSA,MagnusdottirOK,HalldorssonTI,et al.,2018.Towards an individualized nutrition treatment: role of the gastrointestinal microbiome in the interplay between diet and obesity.Curr Obes Rep,7(4):289-293.

[3]AlaqabyARA,GlaskovichAA,KapitonovaEA,et al.,2014.Study the effect of using probiotic (vetlactoflorum) on some of biochemical and immunological parameters of broiler chickens.Basrah J Vet Res,13(1):166-179.

[4]BaderJ,AlbinA,StahlU,2012.Spore-forming bacteria and their utilisation as probiotics.Benefic Microbes,3(1):67-75.

[5]BaiSP,WuAM,DingXM,et al.,2013.Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens.Poult Sci,92(3):663-670.

[6]BeckmanBR,LarsenDA,MoriyamaS,et al.,1998.Insulin-like growth factor-I and environmental modulation of growth during smoltification of spring chinook salmon (Oncorhynchus tshawytscha).Gen Comp Endocrinol,109(3):325-335.

[7]BianXF,WallstromG,DavisA,et al.,2016.Immunoproteomic profiling of antiviral antibodies in new-onset type 1 diabetes using protein arrays.Diabetes,65(1):285-296.

[8]BirbrairA,ZhangT,WangZM,et al.,2013.Role of pericytes in skeletal muscle regeneration and fat accumulation.Stem Cells Dev,22(16):2298-2314.

[9]BuntynJO,SchmidtTB,NisbetDJ,et al.,2016.The role of direct-fed microbials in conventional livestock production.Annu Rev Anim Biosci,4:335-355.

[10]CaoGT,DaiB,WangKL,et al.,2019.Bacillus licheniformis, a potential probiotic, inhibits obesity by modulating colonic microflora in C57BL/6J mice model.J Appl Microbiol,127(3):880-888.

[11]CaporasoJG,KuczynskiJ,StombaughJ,et al.,2010.QIIME allows analysis of high-throughput community sequencing data.Nat Methods,7(5):335-336.

[12]ChenYC,YuYH,2020.Bacillus licheniformis-fermented products improve growth performance and the fecal microbiota community in broilers.Poult Sci,99(3):‍1432-1443.

[13]ChengGY,HaoHH,XieSY,et al.,2014.Antibiotic alternatives: the substitution of antibiotics in animal husbandry?Front Microbiol,5:217.

[14]DasHK,MedhiAK,IslamM,2005.Effect of probiotics on certain blood parameters and carcass characteristics of broiler chicken.Indian J Poult Sci,40(1):83-86.

[15]de BoeverS,VangestelC,de BackerP,et al.,2008.Identification and validation of housekeeping genes as internal control for gene expression in an intravenous LPS inflammation model in chickens.Vet Immunol Immunopathol,122(3-4):312-317.

[16]DjouvinovD,BoichevaS,SimeonovaT,et al.,2005.Effect of feeding Lactina® probiotic on performance, some blood parameters and caecal microflora of mule ducklings.Trakia J Sci,3(2):22-28.

[17]EdgarRC,2013.UPARSE: highly accurate OTU sequences from microbial amplicon reads.Nat Methods,10(10):996-998.

[18]EdgarRC,HaasBJ,ClementeJC,et al.,2011.UCHIME improves sensitivity and speed of chimera detection.Bioinformatics,27(16):2194-2200.

[19]FerrarioC,MilaniC,MancabelliL,et al.,2016.Modulation of theeps-ome transcription ofBifidobacteria through simulation of human intestinal environment.FEMS Microbiol Ecol,92(4):fiw056.

[20]García-CayuelaT,KoranyAM,BustosI,et al.,2014.Adhesion abilities of dairyLactobacillusplantarum strains showing an aggregation phenotype.Food Res Int,57:44-50.

[21]HillC,GuarnerF,ReidG,et al.,2014.Expert consensus document: the international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic.Nat Rev Gastroenterol Hepatol,11(8):506-514.

[22]HirayamaH,SuzukiY,AbeM,et al.,2011.Methylothermus subterraneus sp. Nov., a moderately thermophilic methanotroph isolated from a terrestrial subsurface hot aquifer.Int J Syst Evol Microbiol,61(Pt 11):2646-2653.

[23]KhaliqueA,ZengD,ShoaibM,et al.,2020.Probiotics mitigating subclinical necrotic enteritis (SNE) as potential alternatives to antibiotics in poultry.AMB Express,10:50.

[24]KhanS,RobertsJ,WuSB,2017.Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus.Sci Rep,7:14271.

[25]LammersA,WielandWH,KruijtL,et al.,2010.Successive immunoglobulin and cytokine expression in the small intestine of juvenile chicken.Dev Comp Immunol,34(12):1254-1262.

[26]LinLH,KeFR,ZhanTT,et al.,2014.Effects ofBacilluscoagulans on performance, serum biochemical indices and antioxidant function of yellow broilers.Chin J Anim Nutr,26(12):3806-3813(in Chinese).

[27]LiuJB,YanHL,ZhangY,et al.,2020.Effects of stale maize on growth performance, immunity, intestinal morphology and antioxidant capacity in broilers.Asian-Australas J Anim Sci,33(4):605-614.

[28]LiuSQ,WangLY,LiuGH,et al.,2018.Leucine alters immunoglobulin a secretion and inflammatory cytokine expression induced by lipopolysaccharide via the nuclear factor‍-‍κB pathway in intestine of chicken embryos.Animal,12(9):1903-1911.

[29]LiuX,LiSR,WangZY,et al.,2018.Effects of dietary lipids and clostridium butyricum on chicken volatile flavour compounds.Indian J Anim Res,52(8):1167-1173.

[30]LiuXL,YanH,LvL,et al.,2012.Growth performance and meat quality of broiler chickens supplemented withBacilluslicheniformis in drinking water.Asian-Australas J Anim Sci,25(5):682-689.

[31]LivakKJ,SchmittgenTD,2001.Analysis of relative gene expression data using real-time quantitative PCR and the2-∆∆CTmethod.Methods,25(4):402-408.

[32]LivingstonML,CowiesonAJ,CrespoR,et al.,2020.Effect of broiler genetics, age, and gender on performance and blood chemistry.Heliyon,6(7):e04400.

[33]LokapirnasariWP,al ArifA,SoeharsonoS,et al.,2019.Improves in external and internal egg quality of Japanese quail (Coturnix coturnix japonica) by giving lactic acid bacteria as alternative antibiotic growth promoter.Iran J Microbiol,11(5):406-411.

[34]LouisP,FlintHJ,2009.Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine.FEMS Microbiol Lett,294(1):1-8.

[35]MagočT,SalzbergSL,2011.FLASH: fast length adjustment of short reads to improve genome assemblies.Bioinformatics,27(21):2957-2963.

[36]MassacciFR,LovitoC,TofaniS,et al.,2019.DietarySaccharomyces cerevisiae boulardii CNCM I-1079 positively affects performance and intestinal ecosystem in broilers during aCampylobacter jejuni infection.Microorganisms,7(12):596.

[37]MehdiY,Létourneau-MontminyMP,GaucherML,et al.,2018.Use of antibiotics in broiler production: global impacts and alternatives.Anim Nutr,4(2):170-178.

[38]MichaelidouK,TzovarasA,MissitzisI,et al.,2013.The expression of the CEACAM19 gene, a novel member of the CEA family, is associated with breast cancer progression.Int J Oncol,42(5):1770-1777.

[39]MohanB,KadirvelR,BhaskaranM,et al.,1995.Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers.Br Poultry Sci,36(5):799-803.

[40]MohanB,KadirvelR,NatarajanA,et al.,1996.Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers.Br Poultry Sci,37(2):‍395-401.

[41]PandaAK,RajuMVLN,RamaRSV,et al.,2005.The influence of supplementation ofLactobacillussporogenes on the performance of broilers.Indian J Anim Nutr,22(1):37-40.

[42]PandaAK,RaoSVR,RajuMVLN,et al.,2006.Dietary supplementation ofLactobacillus sporogenes on performance and serum biochemico-lipid profile of broiler chickens.J Poult Sci,43(3):235-240.

[43]RussellDA,RossRP,FitzgeraldGF,et al.,2011.Metabolic activities and probiotic potential of bifidobacteria.Int J Food Microbiol,149(1):88-105.

[44]ScanesCG,2009.Perspectives on the endocrinology of poultry growth and metabolism.Gen Comp Endocrinol,163(1-2):24-32.

[45]SuraiPF,KochishII,FisininVI,2017.Antioxidant systems in poultry biology: nutritional modulation of vitagenes.Eur Poult Sci,81:1-21.

[46]ThapaBR,WaliaA,2007.Liver function tests and their interpretation.Indian J Pediatr,74(7):663-671.

[47]VitalM,HoweAC,TiedjeJM,2014.Revealing the bacterial butyrate synthesis pathways by analyzing (meta) genomic data.mBio,5(2):e00889-14.

[48]WangX.,HuY.,ZhuX,et al.,2023.Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilers.J Animal Sci Biotechnol,14:4.

[49]ZhengJS,WittouckS,SalvettiE,et al.,2020.A taxonomic note on the genusLactobacillus: description of 23 novel genera, emended description of the genusLactobacillus Beijerinck 1901, and union ofLactobacillaceae andLeuconostocaceae.Int J Syst Evol Microbiol,70(4):‍2782-2858.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE