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On-line Access: 2023-11-14

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 ORCID:

Xianghua YAN

https://orcid.org/0000-0003-2238-6218

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.11 P.1014-1026

http://doi.org/10.1631/jzus.B2200621


Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens


Author(s):  Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN

Affiliation(s):  National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology, Huazhong Agricultural University,Wuhan430070,China; more

Corresponding email(s):   xhyan@mail.hzau.edu.cn

Key Words:  Chicken, Aeriscardovia aeriphila, Gut health, Microbiome, Immune function


Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN. Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens[J]. Journal of Zhejiang University Science B, 2023, 24(11): 1014-1026.

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author="Muhammad Zahid FAROOQ, Xinkai WANG, Xianghua YAN",
journal="Journal of Zhejiang University Science B",
volume="24",
number="11",
pages="1014-1026",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200621"
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%0 Journal Article
%T Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens
%A Muhammad Zahid FAROOQ
%A Xinkai WANG
%A Xianghua YAN
%J Journal of Zhejiang University SCIENCE B
%V 24
%N 11
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%D 2023
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2200621

TY - JOUR
T1 - Effects of Aeriscardovia aeriphila on growth performance, antioxidant functions, immune responses, and gut microbiota in broiler chickens
A1 - Muhammad Zahid FAROOQ
A1 - Xinkai WANG
A1 - Xianghua YAN
J0 - Journal of Zhejiang University Science B
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IS - 11
SP - 1014
EP - 1026
%@ 1673-1581
Y1 - 2023
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2200621


Abstract: 
Aeriscardovia aeriphila, also known asBifidobacterium aerophilum, was first isolated from the caecal contents of pigs and the faeces of cotton-top tamarin.Bifidobacterium species play important roles in preventing intestinal infections, decreasing cholesterol levels, and stimulating the immune system. In this study, we isolated a strain of bacteria from the duodenal contents of broiler chickens, which was identified asA. aeriphila, and then evaluated the effects ofA. aeriphila on growth performance, antioxidant functions, immune functions, and gut microbiota in commercial broiler chickens. chickens were orally gavaged withA. aeriphila (1×109 CFU/mL) for 21 d. The results showed thatA. aeriphila treatment significantly increased the average daily gain and reduced the feed conversion ratio (P<0.001). The levels of serum growth hormone (GH) and insulin-like growth factor 1 (IGF-1) were significantly increased followingA. aeriphila treatment (P<0.05). Blood urea nitrogen and aspartate aminotransferase levels were decreased, whereas glucose and creatinine levels increased as a result ofA. aeriphila treatment. Furthermore, the levels of serum antioxidant enzymes, including catalase (P<0.01), superoxide dismutase (P<0.001), and glutathione peroxidase (P<0.05), and total antioxidant capacity (P<0.05) were enhanced followingA. aeriphila treatment.A. aeriphila treatment significantly increased the levels of serum immunoglobulin A (IgA) (P<0.05), IgG (P<0.01), IgM (P<0.05), interleukin-1 (IL-1) (P<0.05), IL-4 (P<0.05), and IL-10 (P<0.05). The broiler chickens in theA. aeriphila group had higher secretory IgA (SIgA) levels in the duodenum (P<0.01), jejunum (P<0.001), and cecum (P<0.001) than those in the control group. The messenger RNA (mRNA) relative expression levels ofIL-10 (P<0.05) andIL-4 (P<0.001) in the intestinal mucosa of chickens were increased, while nuclear factor-‍κB (NF-κB) (P<0.001) expression was decreased in theA. aeriphila group compared to the control group. Phylum-level analysis revealed Firmicutes as the main phylum, followed by Bacteroidetes, in both groups. The data also found thatPhascolarctobacterium andBarnesiella were increased inA. aeriphila-treated group. In conclusion, oral administration ofA. aeriphila could improve the growth performance, serum antioxidant capacity, immune modulation, and gut health of broilers. Our findings may provide important information for the application ofA. aeriphila in poultry production.

Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响

Muhammad Zahid FAROOQ1,2,王新锴1,晏向华1
1华中农业大学动物科学技术学院,农业微生物资源发掘与利用全国重点实验室,湖北洪山实验室,动物育种与健康养殖前沿科学中心,中国武汉市,430070
2巴基斯坦兽医和动物科学大学(Jhang校区)动物科学系,拉合尔54000,巴基斯坦
摘要:Aeriscardovia aeriphila,又名Bifidobacterium aerophilum,是从猪盲肠内容物中分离出的一种双歧杆菌。A. aeriphila在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌,经16S 测序鉴定为 A. aeriphila,然后我们评估了A. aeriphila对商用肉鸡生长性能、抗氧化功能、免疫功能和肠道菌群的影响。实验对21日龄的实验鸡进行了为期3周的灌服A. aeriphila(1×109 CFU/mL)处理。研究结果显示:A. aeriphila处理显著增加了鸡的平均日增重,降低了饲料转化率(P<0.001)。A. aeriphila处理后,血清生长激素(GH)和胰岛素样生长因子1(IGF-1)水平显著升高(P<0.05)。A. aeriphila处理降低了血尿素氮和天门冬氨酸氨基转移酶水平,增加了血糖和肌酐水平。同时,A. aeriphila处理显著提高了血清中抗氧化酶水平(包括过氧化氢酶(P<0.01)、超氧化物歧化酶(P<0.001)和谷胱甘肽过氧化物酶(P<0.05))及总抗氧化能力(P<0.05)。A. aeriphila处理显著增加了血清免疫球蛋白A(IgA)(P<0.05)、IgG(P<0.01)、IgM(P<0.05)、白细胞介素-1(IL-1)(P<0.05)、IL-4(P<0.05)和IL-10(P<0.05)的水平。与对照组相比,A. aeriphila组肉鸡在十二指肠(P<0.01)、空肠(P<0.001)和盲肠(P<0.001)中的分泌型免疫球蛋白A(SIgA)水平显著提高。在肠道黏膜(包括十二指肠、空肠和盲肠)中,A. aeriphila组鸡的IL-10P<0.05)、IL-4P<0.001)和NF-κBP<0.001)信使RNA(mRNA)相对表达水平高于对照组。肠道微生物门水平分析显示,两组的差异菌群主要是厚壁菌门,其次是拟杆菌门。此外,A. aeriphila可提高肠道中PhascolarctobacteriumBarnesiella的丰度。综上所述,灌服A. aeriphila可改善肉鸡的生长性能、血清抗氧化能力、免疫调节和肠道健康。因此,本研究结果可以为A. aeriphila在家禽生产中的应用提供理论依据。

关键词:鸡;Aeriscardovia aeriphila;肠道健康;菌群;免疫功能

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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