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Abstract: To investigate the effect of glycitein, a synthetic soybean isoflavone (ISF), on the intestinal antioxidant capacity, morphology, and cytokine content in young piglets fed oxidized fish oil, 72 4-d-old male piglets were assigned to three treatments. The control group was fed a basal diet containing fresh fish oil, and the other two groups received the same diet except for the substitution with the same dosage of oxidized fish oil alone or with ISF (oxidized fish oil plus ISF). After 21 d of feeding, supplementation of oxidized fish oil increased the levels of malondialdehyde (MDA), oxidized glutathione (GSSG), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), nuclear factor κ B (NF-κB), inducible nitric oxide synthase (iNOS), NO, and Caspase-3 in jejunal mucosa, and decreased the villous height in duodenum and the levels of secretory immunoglobulin A (sIgA) and IL-4 in the jejunal mucosa compared with supplementation with fresh oil. The addition of oxidized fish oil plus ISF partially alleviated this negative effect. The addition of oxidized fish oil plus ISF increased the villous height and levels of sIgA and IL-4 in jejunal mucosa, but decreased the levels of IL-1β and IL-2 in jejunal mucosa (P<0.05) compared with oxidized fish oil. Collectively, these results show that dietary supplementation of ISF could partly alleviate the negative effect of oxidized fish oil by improving the intestinal morphology as well as the antioxidant capacity and immune function in young piglets.

大豆异黄酮对饲喂氧化鱼油新生仔猪肠道抗氧化功能及细胞因子的影响

[1]Andrew, J.S., Griffith, W.H., Mead, J.F., et al., 1960. Toxicity of air-oxidized soybean oil. J. Nutr., 70:199-210.

[2]Aw, T.Y., 1999. Molecular and cellular responses to oxidative stress and changes in oxidation-reduction imbalance in the intestine. Am. J. Clin. Nutr., 70(4):557-565.

[3]Ban, H., Shigemitsu, K., Yamatsuji, T., 2004. Arginine and Leucine regulate p70 S6 kinase and 4E-BP1 in intestinal epithelial cells. Int. J. Mol. Med., 13(4):537-543.

[4]Banan, A., Fields, J.Z., Zhang, Y., et al., 2001. iNOS upregulation mediates oxidant-induced disruption of F-actin and barrier of intestinal monolayers. Am. J. Physiol. Gastrointest Liver Physiol., 280:G1234-G1246.

[5]Blikslager, A.T., Moeser, A.J., Gookin, J.L., et al., 2007. Restoration of barrier function in injured intestinal mucosa. Physiol. Rev., 87(2):545-564.

[6]Burrin, D.G., Stoll, B., Jiang, R., et al., 2000. Minimal enteral nutrient requirements for intestinal growth in neonatal piglets: how much is enough? Am. J. Clin. Nutr., 71:1603-1610.

[7]Cera, K.R., Mahan, D.C., Cross, R.F., et al., 1988. Effect of age, weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine. J. Anim. Sci., 66(2):574-584.

[8]Chamulitrat, W., Skrepnik, N.V., Spitzer. J.J., 1996. Endotoxin-induced oxidative stress in the rat small intestine: role of nitric oxide. Shock, 5(3):217-222.

[9]Chang, R.H., Feng, M.H., Liu, W.H., et al., 1997. Nitric oxide increased interleukin-4 expression in T lymphocytes. Immunology, 90(3):364-369.

[10]Daniel, T., Alexander, M., Hubbard, W.J., et al., 2006. Nitric oxide contributes to the development of a post-injury Th2 T cell phenotype and immune dysfunction. J. Cell. Physiol., 208(2):418-427.

[11]Deng, Q., Xu, J., Yu, B., et al., 2010. Effect of dietary tea polyphenols on growth performance and cell-mediated immune response of post-weaning piglets under oxidative stress. Arch. Anim. Nutr., 64(1):12-21.

[12]de Wilde, A., Lieberherr, M., Colin, C., et al., 2004. A low dose of daidzein acts as an ERβ-selective agonist in trabecular osteoblasts of young female piglets. J. Cell. Physiol., 200(2):253-262.

[13]Dixit, A.K., Bhatnagar, D., Kumar, V., et al., 2012. Antioxidant potential and radioprotective effect of soy isoflavone against gamma irradiation induced oxidative stress. J. Funct. Foods, 4(1):197-206.

[14]Fan, J., Meng, Q., Guo, G., et al., 2010. Effects of early enteral nutrition supplemented with arginine on intestinal mucosal immunity in severely burned mice. Clin. Nutr., 29(1):124-130.

[15]Foti, P., Erba, D., Riso, P., et al., 2005. Comparison between daidzein and genistein antioxidant activity in primary and cancer lymphocytes. Arch. Biochem. Biophys., 433(2):421-427.

[16]Frankel, W.L., Zhang, W., Afonso, J., et al., 1993. Glutamine enhancement of structure and function in transplanted small-intestine in the rat. J. Parenter. Enteral Nutr., 17(1):47-55.

[17]Goerke, M., Eklund, M., Sauer, N., et al., 2012. Standardized ileal digestibilities of crude protein, amino acids, and contents of antinutritional factors, mycotoxins, and isoflavones of European soybean meal imports fed to piglets. J. Anim. Sci., 90(13):4883-4895.

[18]Gookin, J.L., Rhoads, J.M., Argenzio, R.A., 2002. Inducible nitric oxide synthase mediates early epithelial repair of porcine ileum. Am. J. Physiol.-Gastrointest. Liver Physiol., 283(1):G157-G168.

[19]Halliwell, B., Chirico, S., 1993. Lipid peroxidation: its mechanism, measurement, and significance. Am. J. Clin. Nutr., 57(5):715S-724S.

[20]Huang, L., Jiang, Z.Y., Lin, Y.C., et al., 2011. Effects of L-arginine on intestinal development and endogenous arginine-synthesizing enzymes in neonatal pigs. Afr. J. Biotechnol., 10(40):7915-7925.

[21]Jelínková, L., Tučková, L., Cinová, J., et al., 2004. Gliadin stimulates human monocytes to production of IL-8 and TNF-α through a mechanism involving NF-κB. FEBS Lett., 571(1-3):81-85.

[22]Jensen, A.R., Elnif, J., Burrin, D.G., et al., 2001. Development of intestinal immunoglobulin absorption and enzyme activities in neonatal pigs is diet dependent. J. Nutr., 131(12):3259-3265.

[23]Jiang, Z.Y., Jiang, S.Q., Lin, Y.C., et al., 2007. Effects of soybean isoflavone on growth performance, meat quality, and antioxidation in male broilers. Poult. Sci., 86(7):1356-1362.

[24]Jiang, Z.Y., Zhou, G.L., Lin, Y.C., et al., 2011. Effects of soybean isoflavones on in vitro antioxidative capacity of satellite cells of porcine skeletal muscles. Agric. Sci. Chin., 10(1):120-125 (in Chinese).

[25]John, S.A., Wendell, H.G., James, F.M., et al., 1959. Toxicity of air-oxidized soybean oil. J. Nutr., 70(2):199-210.

[26]Kalinski, P., Moser, M., 2005. Consensual immunity: success driven development of T-helper-1 and T-helper-2 responses. Nat. Rev. Immunol., 5(3):251-260.

[27]Kelley, D.S., 2001. Modulation of human immune and inflammatory responses by dietary fatty acids. Nutrition, 17(7-8):669-673.

[28]Kim, M.J., Ryu, G.R., Kang, J.H., et al., 2004. Inhibitory effects of epicatechin on interleukin-1β-induced inducible nitric oxide synthase expression in RINm5F cells and rat pancreatic islets by down-regulation of NF-κB activation. Biochem. Pharmacol., 68(9):1775-1785.

[29]Kudsk, K.A., 2001. Importance of enteral feeding in maintaining gut integrity. Tech. Gastrointest. Endosc., 3(1):2-8.

[30]Lackeyram, D., Mine, Y., Widowski, T., et al., 2012. The in vivo infusion of hydrogen peroxide induces oxidative stress and differentially affects the activities of small intestinal carbohydrate digestive enzymes in the neonatal pig. J. Anim. Sci., 90(Suppl. 4):418-420.

[31]Lee, C.H., Yang, L., Xu, J.Z., et al., 2005. Relative antioxidant activity of soybean isoflavones and their glycosides. Food Chem., 90(4):735-741.

[32]Leitch, G.J., He, Q., 1999. Reactive nitrogen and oxygen species ameliorate experimental cryptosporidiosis in the neonatal BALB/c mouse model. Infect. Immun., 67:5885-5891.

[33]Li, D.F., Nelssen, J.L., Reddy, P.G., et al., 1990. Transient hypersensitivity to soybean meal in early-weaned pig. J. Anim. Sci., 68(6):1790-1799.

[34]Li, D.F., Nelssen, J.L., Reddy, P.G., et al., 1991. Measuring suitability of soybean products for early-weaned pigs with immunological criteria. J. Anim. Sci., 69(8):3299-3307.

[35]Li, Y., Ahmed, F., Ali, S., et al., 2005. Inactivation of nuclear factor κB by soy isoflavone genistein contributes to increased apoptosis induced by chemotherapeutic agents in human cancer cells. Cancer Res., 65(15):6934-6943.

[36]Liu, J.B., Chang, S.K.C., Wiesenborn, D., 2005. Antioxidant properties of soybean isoflavone extract and tofu in vitro and in vivo. J. Agric. Food. Chem., 53(6):2333-2340.

[37]Liu, J.F., Lee, Y.W., 1998. Vitamin C supplementation restores the impaired Vitamin E status of guinea pigs fed oxidized frying oil. J. Nutr., 128(1):116-122.

[38]Maeda, T., Miyazono, Y., Ito, K., et al., 2010. Oxidative stress and enhanced paracellular permeability in the small intestine of methotrexate-treated rats. Cancer Chemother. Pharmacol., 65(6):1117-1123.

[39]Mau, M., Kalbe, C., Viergutz, T., et al., 2008. Effects of dietary isoflavones on proliferation and DNA integrity of myoblasts derived from newborn piglets. Pediatr. Res., 63(1):39-45.

[40]Mercer, D.W., Smith, G.S., Cross, J.M., et al., 1996. Effects of lipopolysaccharide on intestinal injury: potential role of nitric oxide and lipid peroxidation. J. Surg. Res., 63(1):185-192.

[41]Moreto, M., Perez-Bosque, A., 2009. Dietary plasma proteins, the intestinal immune system, and the barrier functions of the intestinal mucosa. J. Anim. Sci., 87(14_Suppl.):E92-E100.

[42]Morimoto, M., Watanabe, T., Yamori, M., et al., 2009. Isoflavones regulate innate immunity and inhibit experimental colitis. J. Gastroenterol. Hepatol., 24(6):1123-1129.

[43]Otani, K., Shimizu, S., Chijiiwa, K., et al., 2000. Administration of bacterial lipopolysaccharide to rats induces heme oxygenase-1 and formation of antioxidant bilirubin in the intestinal mucosa. Digest. Dis. Sci., 45(12):2313-2319.

[44]Ozacmak, H.S., Ozacmak, V.H., Barut, F., et al., 2014. Pretreatment with mineralocorticoid receptor blocker reduces intestinal injury induced by ischemia and reperfusion: involvement of inhibition of inflammatory response, oxidative stress, nuclear factor κB, and inducible nitric oxide synthase. J. Surg. Res., 191(2):350-361.

[45]Pampusch, M.S., Bennaars, A.M., Harsch, S., et al., 1998. Inducible nitric oxide synthase expression in porcine immune cells. Vet. Immunol. Immunopathol., 61:279-289.

[46]Parikh, N.A., Katsetos, C.D., Ashraf, Q.M., et al., 2003. Hypoxia-induced Caspase-3 activation and DNA fragmentation in cortical neurons of newborn piglets: role of nitric oxide. Neurochem. Res., 28(9):1351-1357.

[47]Pruett, S.B., 2003. Stress and the immune system. Pathophysiology, 9(3):133-153.

[48]Science and Technology Ministry of China, 2006. The guiding suggestion about treating experimental animals amicably. Document No. 398.

[49]Shang, H., Tsai, H., Chiu, W., et al., 2004. Effects of Arginine supplementation on mucosal immunity in rats with septic peritonitis. Clin. Nutr., 23(4):561-569.

[50]Slater, T.F., 1984. Free radical mechanisms in tissue injury. Biochem. J., 222(1):1-15.

[51]Taylor-Robinson, A.W., 1997. Inhibition of IL2 production by nitric oxide: a novel self regulatory mechanism for Th1 cell proliferation. Immunol. Cell Biol., 75(2):167-175.

[52]Valachovicova, T., Slivova, V., Bergman, H., et al., 2005. Soy isoflavones suppress invasiveness of breast cells by the inhibition of NF-κB/AP-1-dependent and independent pathways. Int. J. Oncol., 25(5):1389-1395.

[53]VázquezAñón, M., Jenkins, T., 2007. Effects of feeding oxidized fat with or without dietary antioxidants on nutrient digestibility, microbial nitrogen and fatty acid metabolism. J. Dairy Sci., 90(9):4361-4867.

[54]Xi, P.B., Jiang, Z.Y., Zheng, C.T., et al., 2011. Regulation of protein metabolism by glutamine: implications for nutrition and health. Front. Biosci., 16(1):578-597.

[55]Yousef, M.I., Kamel, K.I., Esmail, A.M., et al., 2004. Antioxidant activities and lipid lowering effects of isoflavone in male rabbits. Food Chem. Toxicol., 42(9):1497-1503.

[56]Ypsilantis, P., Tentes, L., Lambropoulou, M., et al., 2008. Prophylaxis with mesna prevents oxidative stress induced by ischemia reperfusion in the intestine via inhibition of nuclear factor-κB activation. J. Gastroenterol. Hepatol., 23(2):328-335.

[57]Yuan, S.B., 2007. Impacts of Oxidative Stress on Piglets and the Anti-stress Effect and Mechanism of Selenium. PhD Thesis, Sichuan Agriculture University, China (in Chinese).

[58]Zhan, X., Qie, Y., Wang, M., et al., 2011. Selenomethionine: an effective selenium source for sow to improve Se distribution, antioxidant status, and growth performance of pig offspring. Biol. Trace Elem. Res., 142(3):481-491.