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CLC number: S512.3

On-line Access: 2016-10-02

Received: 2015-11-11

Revision Accepted: 2016-04-17

Crosschecked: 2016-09-11

Cited: 2

Clicked: 1400

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Bao-jian Guo

http://orcid.org/0000-0003-4147-7414

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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.10 P.763-774

10.1631/jzus.B1500277


A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)


Author(s):  Bao-jian Guo, Jun Wang, Shen Lin, Zheng Tian, Kai Zhou, Hai-ye Luan, Chao Lyu, Xin-zhong Zhang, Ru-gen Xu

Affiliation(s):  Jiangsu Key Laboratory of Crop Genetics and Physiology / Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China; more

Corresponding email(s):   rgxu@yzu.edu.cn

Key Words:  Barley, AS2/LOB gene family, Phylogenetic tree, Expression pattern


Bao-jian Guo, Jun Wang, Shen Lin, Zheng Tian, Kai Zhou, Hai-ye Luan, Chao Lyu, Xin-zhong Zhang, Ru-gen Xu. A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)[J]. Journal of Zhejiang University Science B, 2016, 17(10): 763-774.

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author="Bao-jian Guo, Jun Wang, Shen Lin, Zheng Tian, Kai Zhou, Hai-ye Luan, Chao Lyu, Xin-zhong Zhang, Ru-gen Xu",
journal="Journal of Zhejiang University Science B",
volume="17",
number="10",
pages="763-774",
year="2016",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500277"
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%0 Journal Article
%T A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)
%A Bao-jian Guo
%A Jun Wang
%A Shen Lin
%A Zheng Tian
%A Kai Zhou
%A Hai-ye Luan
%A Chao Lyu
%A Xin-zhong Zhang
%A Ru-gen Xu
%J Journal of Zhejiang University SCIENCE B
%V 17
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%P 763-774
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500277

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T1 - A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)
A1 - Bao-jian Guo
A1 - Jun Wang
A1 - Shen Lin
A1 - Zheng Tian
A1 - Kai Zhou
A1 - Hai-ye Luan
A1 - Chao Lyu
A1 - Xin-zhong Zhang
A1 - Ru-gen Xu
J0 - Journal of Zhejiang University Science B
VL - 17
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SP - 763
EP - 774
%@ 1673-1581
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500277


Abstract: 
ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) genes are a family of plant specific transcription factors, which play an important role in the regulation of plant lateral organ development and metabolism. However, a genome-wide analysis of the AS2/LOB gene family is still not available for barley. In the present study, 24 AS2-like (ASL)/LOB domain (LBD) genes were identified based on the barley (Hordeum vulgare L.) genome sequence. A phylogenetic tree of ASL/LBD proteins from barley, Arabidopsis, maize, and rice was constructed. The ASL/LBD genes were classified into two classes, class I and class II, which were divided into five and two subgroups, respectively. Genes homologous in barley and Arabidopsis were analyzed. In addition, the structure and chromosomal locations of the genes were analyzed. Expression profiles indicated that barley HvASL/LBD genes exhibit a variety of expression patterns, suggesting that they are involved in various aspects of physiological and developmental processes. This genome-wide analysis of the barley AS2/LOB gene family contributes to our understanding of the functions of the AS2/LOB gene family.

大麦ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES(AS2/LOB)基因家族的全基因组分析

目的:从大麦全基因组中鉴定AS2/LOB基因家族,并进行基因进化、基因结构、染色体定位以及组织、表达分析,为大麦ASL/LBD基因进一步功能研究与鉴定奠定基础。
创新点:首次在大麦全基因组水平上分析AS2/LOB基因家族,并对部分基因的功能进行预测和分析。
方法:利用大麦基因组数据库,通过生物信息学手段,鉴定大麦AS2/LOB基因家族成员;采用MEGA6软件进行系统进化树分析;利用GSDS及MapDraw工具进行基因结构及染色体定位分析;利用已有的大麦RNAseq数据进行组织表达谱分析。
结论:通过全基因组分析,大麦AS2/LOB家族基因包括24个成员,在进化上分为两大类,7个亚家族,分布于大麦7条染色体上,组织表达模式具有多样性,与已经报道的ASL/LBD基因具有良好的同源性。这些信息为大麦AS2/LOB基因家族的功能分析奠定了基础。

关键词:大麦;AS2/LOB基因家族;进化树;表达模式

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[41]Table S1 Amino acid sequence of the ASL/LBD genes in barley

[42]Fig. S1 Promoter analysis of HvASL12/LBD12 and HvASL14/ LBD19 genes

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