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Journal of Zhejiang University SCIENCE A 2003 Vol.4 No.1 P.86~94

http://doi.org/10.1631/jzus.2003.0086


Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups' binding with human aFGF and bFGF


Author(s):  WU Xiao-feng, XU Ya-xiang, SHEN Guo-xin, KAMEI Kaeko, TAKANO Ryo, HARA Saburo

Affiliation(s):  College of Animal Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China; more

Corresponding email(s):   wuxiaofeng@zju.edu.cn

Key Words:  Surface plasmon resonance analyses, Heparin, Sulfate groups, Human aFGF and bFGF


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WU Xiao-feng, XU Ya-xiang, SHEN Guo-xin, KAMEI Kaeko, TAKANO Ryo, HARA Saburo. Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups' binding with human aFGF and bFGF[J]. Journal of Zhejiang University Science A, 2003, 4(1): 86~94.

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author="WU Xiao-feng, XU Ya-xiang, SHEN Guo-xin, KAMEI Kaeko, TAKANO Ryo, HARA Saburo",
journal="Journal of Zhejiang University Science A",
volume="4",
number="1",
pages="86~94",
year="2003",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2003.0086"
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%0 Journal Article
%T Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups' binding with human aFGF and bFGF
%A WU Xiao-feng
%A XU Ya-xiang
%A SHEN Guo-xin
%A KAMEI Kaeko
%A TAKANO Ryo
%A HARA Saburo
%J Journal of Zhejiang University SCIENCE A
%V 4
%N 1
%P 86~94
%@ 1869-1951
%D 2003
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2003.0086

TY - JOUR
T1 - Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups' binding with human aFGF and bFGF
A1 - WU Xiao-feng
A1 - XU Ya-xiang
A1 - SHEN Guo-xin
A1 - KAMEI Kaeko
A1 - TAKANO Ryo
A1 - HARA Saburo
J0 - Journal of Zhejiang University Science A
VL - 4
IS - 1
SP - 86
EP - 94
%@ 1869-1951
Y1 - 2003
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2003.0086


Abstract: 
Human acidic and basic fibroblast growth factors (aFGF and bFGF) are classic and well characterized members of the heparin-binding growth factor family. heparin is generally thought to play an extremely important role in regulating aFGF and bFGF bioactivities through its strong binding with them. In order to unravel the mechanism of the interactions between heparin and FGFs, and evaluate the importance of heparin sulfate groups' binding with FGFs, surface plasmon resonance analyses were performed using IAsys Cuvettes System. heparin and its regioselectively desulfated derivatives were immobilized on the cuvettes. aFGF and bFGF solutions with different concentrations were pipetted into the cuvettes and the progress of the interaction was monitored in real-time by Windows-based software, yielding kinetic and equilibrium constants for these interactions. In addition, in order to reduce the delicate difference among the cuvettes, inhibition analyses of mixture of FGFs and immobilized native heparin by modified heparins were also done. The data from these two methods were similar, indicating that all sulfate groups at 2-O, 6-O and N- in heparin were required for the binding to aFGF; and that their contribution to the binding was in the order 2-O, N- and 6-O-sulfate group. In contrast, definite contribution of the 6-O-sulfate group to the binding with bFGF was most apparent, while the other two sulfate groups appeared to be necessary in the order 2-O and N-sulfate group. These methods established here can be used for analysing the effect of sulfate groups in heparin on the binding with other human FGF members or other heparin-binding proteins.

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