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CLC number: TS201.6

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Received: 2007-06-21

Revision Accepted: 2007-09-13

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.12 P.900-905


Development and evaluation of immunoassay for zeranol in bovine urine

Author(s):  Liu Yuan, Zhang Cun-zhen, Yu Xiang-yang, Zhang Zhi-yong, Zhang Xiao, Liu Rong-rong, Liu Xian-jin, Gong Zhen-ming

Affiliation(s):  Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; more

Corresponding email(s):   jaasliu@jaas.ac.cn

Key Words:  Zeranol, Enzyme linked immunosorbent assay (ELISA), Bovine urine

Liu Yuan, Zhang Cun-zhen, Yu Xiang-yang, Zhang Zhi-yong, Zhang Xiao, Liu Rong-rong, Liu Xian-jin, Gong Zhen-ming. Development and evaluation of immunoassay for zeranol in bovine urine[J]. Journal of Zhejiang University Science B, 2007, 8(12): 900-905.

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author="Liu Yuan, Zhang Cun-zhen, Yu Xiang-yang, Zhang Zhi-yong, Zhang Xiao, Liu Rong-rong, Liu Xian-jin, Gong Zhen-ming",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Development and evaluation of immunoassay for zeranol in bovine urine
%A Liu Yuan
%A Zhang Cun-zhen
%A Yu Xiang-yang
%A Zhang Zhi-yong
%A Zhang Xiao
%A Liu Rong-rong
%A Liu Xian-jin
%A Gong Zhen-ming
%J Journal of Zhejiang University SCIENCE B
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%P 900-905
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0900

T1 - Development and evaluation of immunoassay for zeranol in bovine urine
A1 - Liu Yuan
A1 - Zhang Cun-zhen
A1 - Yu Xiang-yang
A1 - Zhang Zhi-yong
A1 - Zhang Xiao
A1 - Liu Rong-rong
A1 - Liu Xian-jin
A1 - Gong Zhen-ming
J0 - Journal of Zhejiang University Science B
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SP - 900
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%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2007.B0900

A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1:5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml , and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation (CVs) were in the range of 82%~127% and 3.5%~8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol (ranging from 0.2 to 10 μg/ml) were detected by the ELISA and liquid chromatography (LC) method, and good correlations were obtained between the two methods (R2=0.9643). We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an alternative for the conventional LC method for zeranol in bovine urine.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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