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Received: 2004-10-15

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.2 P.83~86

10.1631/jzus.2005.B0083


Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division


Author(s):  CUI Xiao-feng, LI Yun-qin, HU Dong-wei, ZHOU Xue-ping

Affiliation(s):  School of Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   zzhou@zju.edu.cn

Key Words:  Tomato yellow leaf curl China virus (TYLCCNV), DNA&beta


CUI Xiao-feng, LI Yun-qin, HU Dong-wei, ZHOU Xue-ping. Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division[J]. Journal of Zhejiang University Science B, 2005, 6(2): 83~86.

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author="CUI Xiao-feng, LI Yun-qin, HU Dong-wei, ZHOU Xue-ping",
journal="Journal of Zhejiang University Science B",
volume="6",
number="2",
pages="83~86",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0083"
}

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%T Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division
%A CUI Xiao-feng
%A LI Yun-qin
%A HU Dong-wei
%A ZHOU Xue-ping
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 2
%P 83~86
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0083

TY - JOUR
T1 - Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division
A1 - CUI Xiao-feng
A1 - LI Yun-qin
A1 - HU Dong-wei
A1 - ZHOU Xue-ping
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 2
SP - 83
EP - 86
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0083


Abstract: 
An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNA&beta;) is required to induce typical symptoms in host plants. DNA&beta; encodes a single gene (termed βC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing the βc1 gene of a DNA&beta; associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. transgenic plants expressing βC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the βC1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 protein was discussed.

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Reference

[1] Briddon, R.W., Mansoor, S., Bedford, I.D., Pinner, M.S., Saunders, K., Stanley, J., Zafar, Y., Malik, K.A., Markham, P.G., 2001. Identification of DNA components required for induction of cotton leaf curl disease. Virology, 285:234-243.

[2] Briddon, R.W., Bull, S.E., Amin, I., Idris, A.M., Mansoor, S., Bedford, I.D., Dhawan, P., Rishi, N., Siwatch, S.S., Abdel-Salam, A.M., Brown, J.K., Zafar, Y., Markham, P.G., 2003. Diversity of DNAβ, a satellite molecule associated with some monopartite begomoviruses. Virology, 312:106-121.

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[4] Hanley-Bowdoin, L., Settlage, S.B., Orozco, B.M., Nagar, S., Robertson, D., 1999. Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation. Cri. Rev. Plant Sci., 18:71-106.

[5] Jose, J., Usha, R., 2003. Bhendi yellow vein mosaic disease in India is caused by association of a DNAβ satellite with a Begomovirus. Virology, 305:310-317.

[6] Saunders, K., Bedford, I.D., Briddon, R.W., Markham, P.G., Wong, S.M., Stanley, J., 2000. A unique virus complex causes ageratum yellow vein disease. Proc. Natl. Acad. Sci. USA, 97:6890-6895.

[7] Saunders, K., Norman, A., Gucciardo, S., Stanley, J., 2004. The DNAβ satellite component associated with ageratum yellow vein disease encodes an essential pathogenicity protein (βC1). Virology, 324:37-47.

[8] Zhou, X.P., Xie, Y., Tao, X.R., Zhang, Z.K., Li, Z.H., Fauquet, C.M., 2003. Characterization of DNA associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A. J. Gen. Virol., 84:237-247.

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