CLC number: Q27
On-line Access:
Received: 2004-09-17
Revision Accepted: 2004-11-18
Crosschecked: 0000-00-00
Cited: 3
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SUN Lei, NIWA Koichi, LIN Jian-zhong, KARINO Takeshi. Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft[J]. Journal of Zhejiang University Science B, 2005, 6(2): 79-82.
@article{title="Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft",
author="SUN Lei, NIWA Koichi, LIN Jian-zhong, KARINO Takeshi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="2",
pages="79-82",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0079"
}
%0 Journal Article
%T Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft
%A SUN Lei
%A NIWA Koichi
%A LIN Jian-zhong
%A KARINO Takeshi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 2
%P 79-82
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0079
TY - JOUR
T1 - Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft
A1 - SUN Lei
A1 - NIWA Koichi
A1 - LIN Jian-zhong
A1 - KARINO Takeshi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 2
SP - 79
EP - 82
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0079
Abstract: High blood pressure (hypertension) is implicated in the development of atherosclerosis. Blood vessels are constantly subjected to stretch due to blood pressure and changes in stretch usually instigate adaptive vascular remodeling, including abnormal growth and proliferation of vascular smooth muscle cells (VSMCs) as well as extracellular matrix (ECM). In this experiment, we used bovine aortic endothelial cells and smooth muscle cells (EC-SMC) co-cultured ePTFE vascular grafts subjected to normal atmospheric pressure (as a control), and 100 mmHg hydrostatic pressure for 7 d. The increase of cell layer thickness was observed. When measured, the cell layer thickness increased by 116.2%. The increase of collagen (Type IV) synthesis was also observed in the immunohistochemistry assay. When stained with toluidine blue, the cells showed metachromatic phenomenon.
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