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Received: 2004-09-17

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.2 P.79~82

10.1631/jzus.2005.B0079


Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft


Author(s):  SUN Lei, NIWA Koichi, LIN Jian-zhong, KARINO Takeshi

Affiliation(s):  Department of Mechanics, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   slsunlei@yahoo.com.cn

Key Words:  Pressure, ePTFE graft, EC-SMC co-culture, Collagen, Proliferation, Metachromatic


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SUN Lei, NIWA Koichi, LIN Jian-zhong, KARINO Takeshi. Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft[J]. Journal of Zhejiang University Science B, 2005, 6(2): 79~82.

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author="SUN Lei, NIWA Koichi, LIN Jian-zhong, KARINO Takeshi",
journal="Journal of Zhejiang University Science B",
volume="6",
number="2",
pages="79~82",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0079"
}

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%T Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft
%A SUN Lei
%A NIWA Koichi
%A LIN Jian-zhong
%A KARINO Takeshi
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 2
%P 79~82
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0079

TY - JOUR
T1 - Cellular growth under hydrostatic pressure using bovine aortic EC-SMC co-cultured ePTFE vascular graft
A1 - SUN Lei
A1 - NIWA Koichi
A1 - LIN Jian-zhong
A1 - KARINO Takeshi
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 2
SP - 79
EP - 82
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0079


Abstract: 
High blood pressure (hypertension) is implicated in the development of atherosclerosis. Blood vessels are constantly subjected to stretch due to blood pressure and changes in stretch usually instigate adaptive vascular remodeling, including abnormal growth and proliferation of vascular smooth muscle cells (VSMCs) as well as extracellular matrix (ECM). In this experiment, we used bovine aortic endothelial cells and smooth muscle cells (EC-SMC) co-cultured ePTFE vascular grafts subjected to normal atmospheric pressure (as a control), and 100 mmHg hydrostatic pressure for 7 d. The increase of cell layer thickness was observed. When measured, the cell layer thickness increased by 116.2%. The increase of collagen (Type IV) synthesis was also observed in the immunohistochemistry assay. When stained with toluidine blue, the cells showed metachromatic phenomenon.

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Reference

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[5] Ozaki, T., Iizuka, K., Suzuki, M., Murakami, T., Kitabatake, A., Kawaguchi, H., 1999. Threshold-dependent DNA synthesis by pure pressure in human aortic smooth muscle cells: Gialpha-dependent and -independent pathways. Biochemical and Biophysical Research Communications, 256:212-217.

[6] Williams, S.K., Rose, D.G., Jarrell, B.E., 1994. Microvascular endothelial cell sodding of ePTFE vascular grafts: improved patency and stability of the cellular lining. Journal of Biomedical Mater Research, 28:203-212.

[7] Willis, A.I., Pierre-Paul, D., Sumpio, B.E. Gahtan, V., 2004. Vascular smooth muscle cell migration: Current research and clinical implications. Vascular and Endovascular Surgery, 38:11-23

[8] Xing, Y., He, Z., Warnock, J.N. Hilbert, S.L., Yoganathan, A.P., 2004. Effects of constant static pressure on the biological properties of porcine aortic valve leaflets. Annals of Biomedical Engineering, 32:555-562.

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