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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.8 P.623~629

http://doi.org/10.1631/jzus.B0820046


Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture


Author(s):  Li-jie FAN, Takeshi KARINO

Affiliation(s):  Laboratory of Biofluid Dynamics, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan; more

Corresponding email(s):   karino@es.hokudai.ac.jp

Key Words:  Endothelial cells (ECs), Smooth muscle cells (SMCs), Monocyte, THP-1 cells, Low-density lipoprotein (LDL), Adhesion, Transendothelial migration, Serum concentration


Li-jie FAN, Takeshi KARINO. Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture[J]. Journal of Zhejiang University Science B, 2008, 9(8): 623~629.

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author="Li-jie FAN, Takeshi KARINO",
journal="Journal of Zhejiang University Science B",
volume="9",
number="8",
pages="623~629",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820046"
}

%0 Journal Article
%T Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture
%A Li-jie FAN
%A Takeshi KARINO
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 8
%P 623~629
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820046

TY - JOUR
T1 - Effect of serum concentration on adhesion of monocytic THP-1 cells onto cultured EC monolayer and EC-SMC co-culture
A1 - Li-jie FAN
A1 - Takeshi KARINO
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 8
SP - 623
EP - 629
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820046


Abstract: 
Background: The adhesion of monocytes to the endothelium following accumulation of low-density lipoprotein (LDL) in subendothelial spaces is an important step in the development of intimal hyperplasia in arterially implanted vein grafts and atherosclerosis in both animals and humans. However, it is not well known how serum factors affect the adhesion of monocytes. Methods: We have studied the effect of fetal calf serum (FCS), which we considered a source of LDL, on the adhesion of monocytes to endothelial cells (ECs) by using human monocytic THP-1 cells and both a monolayer of cultured bovine aortic endothelial cells (EC monoculture) and a co-culture with bovine aortic smooth muscle cells (EC-SMC co-culture). Results: It was found that the addition of FCS to the medium greatly affected the adhesion of THP-1 cells, and the higher the concentration of FCS in the medium, the greater the adhesion of THP-1 cells to endothelial cells. adhesion of THP-1 cells to an EC-SMC co-culture was approximately twofold greater than that to an EC monoculture, and after adhering to endothelial cells, many THP-1 cells transmigrated into the layer of smooth muscle cells. Conclusion: The results suggest that the elevation of the LDL (cholesterol) level in blood provides a favorable condition for the development of intimal hyperplasia and atherosclerosis by promoting the adhesion of monocytes to the endothelium and their subsequent migration into subendothelial spaces.

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Reference

[1] Back, M.R., Carew, T.E., Schmid-Schoenbein, G.W., 1995. Deposition pattern of monocytes and fatty streak development in hypercholesterolemic rabbits. Atherosclerosis, 116(1):103-115.

[2] Berliner, J.A., Territo, M.C., Sevanian, A., Ramin, S., Kim, J.A., Bamshad, B., Esterson, M., Fogelman, A.M., 1990. Minimally modified low density lipoprotein stimulates monocyte endothelial interactions. J. Clin. Invest., 85(4):1260-1266.

[3] Cai, Q.J., Lanting, L., Natarajan, R., 2004. Interaction of monocytes with vascular smooth muscle cells regulate monocyte survival and differentiation through distant pathways. Atheriosclerosis Thrombosis and Vascular Biology, 24(12):2263-2270.

[4] Cushing, S.D., Berliner, J.A., Valente, A.J., Territo, M.C., Navab, M., Parhami, F., Gerrity, R., Schwartz, C.J., Fogelman, A.M., 1990. Minimally modified low density lipoprotein induces monocyte chemotactic protein 1 in human endothelial cells and smooth muscle cells. Proc. Natl. Acad. Sci. USA, 87(13):5134-5138.

[5] Gerrity, R.G., 1981a. The role of the monocyte in atherogenesis. I: Transition of blood-bone monocytes into foam cells in fatty lesions. Am. J. Pathol., 103:181-190.

[6] Gerrity, R.G., 1981b. The role of the monocyte in atherogenesis. II: Migration of foam cells from atherosclerotic lesions. Am. J. Pathol., 103:191-200.

[7] Itoh, H., Komori, K., Funahashi, S., Okadome, K., Sugimachi, K., 1994. Intimal hyperplasia of experimental autologous vein graft in hyperlipidemic rabbits with poor distal runoff. Atherosclerosis, 110(2):259-270.

[8] Kinard, F., Jaworshi, K., Sergent-Engelen, T., Goldstein, D., van Veldhoven, P.P., Holvoet, P., Trouet, A., Schneider, Y.J., Remacle, C., 2001. Smooth muscle cells influence monocyte response to LDL as well as their adhesion and transmigration in a coculture model of the arterial wall. J. Vasc. Res., 38(5):479-491.

[9] Malinauskas, R.A., Herrmann, R.A., Truskey, G.A., 1995. The distribution of intimal white blood cells in the normal rabbit aorta. Atherosclerosis, 115(2):147-163.

[10] Michison, M.J., Carpenter, K.L.H., Ball, R.Y., 1990. The Role of Macrophages in Human Atherosclerosis. In: Glagov, S., Newman, W.P., Schaffer, S.A. (Eds.), Pathobiology of the Human Atherosclerotic Plaque. Springer-Verlag, New York, p.121-128.

[11] Schwenke, D.C., Carew, T.E., 1989. Initiation of atherosclerotic lesions in cholesterol-fed rabbits. I. Focal increases in arterial LDL concentration precedes development of fatty streak lesions. Arteriosclerosis, 9:895-907.

[12] Stark, V.K., Warner, T.F., Hoch, J.R., 1997. An ultrastructural study of progressive intimal hyperplasia in rat vein grafts. J. Vasc. Surg., 26(1):94-103.

[13] Steinberg, D., Lewis, A., 1997. Conner Memorial Lecture. Oxidative modification of LDL and atherogenesis. Circulation, 95:1062-1071.

[14] Zwolak, R.M., Kirkman, T.R., Clowes, A.W., 1989. Atherosclerosis in rabbit vein Grafts. Arteriosclerosis, 9:374-379.

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