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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.9 P.702~706

10.1631/jzus.B0920066


PI-3 kinase pathway can mediate the effect of TGF-β1 in inducing the expression of SHARP-2 in LLC-PK1 cells


Author(s):  Zhang-fei SHOU, Qin ZHOU, Jie-ru CAI, Jiang-hua CHEN, Kazuya YAMADA, Kaoru MIYAMOTO

Affiliation(s):  Kidney Disease Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   zfshou@zju.edu.cn

Key Words:  Transforming growth factor (TGF)-&beta, 1, Split- and hairy-related protein-2 (SHARP-2), Phosphoinositide 3-kinases (PI-3) kinase pathway, LY294002


Zhang-fei SHOU, Qin ZHOU, Jie-ru CAI, Jiang-hua CHEN, Kazuya YAMADA, Kaoru MIYAMOTO. PI-3 kinase pathway can mediate the effect of TGF-β1 in inducing the expression of SHARP-2 in LLC-PK1 cells[J]. Journal of Zhejiang University Science B, 2009, 10(9): 702~706.

@article{title="PI-3 kinase pathway can mediate the effect of TGF-β1 in inducing the expression of SHARP-2 in LLC-PK1 cells",
author="Zhang-fei SHOU, Qin ZHOU, Jie-ru CAI, Jiang-hua CHEN, Kazuya YAMADA, Kaoru MIYAMOTO",
journal="Journal of Zhejiang University Science B",
volume="10",
number="9",
pages="702~706",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920066"
}

%0 Journal Article
%T PI-3 kinase pathway can mediate the effect of TGF-β1 in inducing the expression of SHARP-2 in LLC-PK1 cells
%A Zhang-fei SHOU
%A Qin ZHOU
%A Jie-ru CAI
%A Jiang-hua CHEN
%A Kazuya YAMADA
%A Kaoru MIYAMOTO
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 9
%P 702~706
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920066

TY - JOUR
T1 - PI-3 kinase pathway can mediate the effect of TGF-β1 in inducing the expression of SHARP-2 in LLC-PK1 cells
A1 - Zhang-fei SHOU
A1 - Qin ZHOU
A1 - Jie-ru CAI
A1 - Jiang-hua CHEN
A1 - Kazuya YAMADA
A1 - Kaoru MIYAMOTO
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 9
SP - 702
EP - 706
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920066


Abstract: 
We aim to investigate the effect of transforming growth factor (TGF)-&beta;1 on the expression of enhancer of split- and hairy-related protein-2 (SHARP-2) messenger RNA (mRNA) and its signaling pathway. In this study, several cell lines including LLC-PK1 (a porcine kidney tubular epithelial cell line), MDCK (Madin-Darby canine kidney) and CTLL-2 (cytotoxic T-lymphocyte line) were treated with recombinant human TGF-β1, and a series of experiments were carried out, involving Northern blot analysis of total RNA from these cells. Further, several specific chemical inhibitors were applied before TGF-β1 treatment to probe the signaling pathway. The results showed that TGF-β1 can significantly up-regulate SHARP-2 mRNA expression in the LLC-PK1 cell line. The peak level of induction was found 2 h after TGF-β1 stimulation. While one phosphoinositide 3-kinases (PI-3) kinase inhibitor, LY294002, completely blocked the effect of TGF-β1 on SHARP-2 mRNA expression in LLC-PK1 cells at a low concentration, other inhibitors, including PD98059, staurosporine, AG490, wortmannin, okadaic acid and rapamycin, had no effect. The effect of LY294002 was dose-dependent. We conclude that, in LLC-PK1 cells at least, TGF-β1 can effectively induce the SHARP-2 mRNA expression and that the PI-3 kinase pathway can mediate this effect.

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Reference

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