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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.9 P.659~667

10.1631/jzus.B0920110


Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro


Author(s):  Fei-yan LI, Xi-sheng XIE, Jun-ming FAN, Zi LI, Jiang WU, Rong ZHENG

Affiliation(s):  Department of Nephrology, West China Hospital of Sichuan University, Chengdu 610041, China; more

Corresponding email(s):   junmingfan2007@yahoo.com.cn

Key Words:  Hydraulic pressure, Tubular epithelial-myofibroblast transdifferentiation, Transforming growth factor-&beta, 1 (TGF-&beta, 1)


Fei-yan LI, Xi-sheng XIE, Jun-ming FAN, Zi LI, Jiang WU, Rong ZHENG. Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro[J]. Journal of Zhejiang University Science B, 2009, 10(9): 659~667.

@article{title="Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro",
author="Fei-yan LI, Xi-sheng XIE, Jun-ming FAN, Zi LI, Jiang WU, Rong ZHENG",
journal="Journal of Zhejiang University Science B",
volume="10",
number="9",
pages="659~667",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0920110"
}

%0 Journal Article
%T Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro
%A Fei-yan LI
%A Xi-sheng XIE
%A Jun-ming FAN
%A Zi LI
%A Jiang WU
%A Rong ZHENG
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 9
%P 659~667
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920110

TY - JOUR
T1 - Hydraulic pressure inducing renal tubular epithelial-myofibroblast transdifferentiation in vitro
A1 - Fei-yan LI
A1 - Xi-sheng XIE
A1 - Jun-ming FAN
A1 - Zi LI
A1 - Jiang WU
A1 - Rong ZHENG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 9
SP - 659
EP - 667
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920110


Abstract: 
Objective: The effects of hydraulic pressure on renal tubular epithelial-myofibroblast transdifferentiation (TEMT) were investigated. Methods: We applied hydraulic pressure (50 cmH2O) to normal rat kidney tubular epithelial cells (NRK52E) for different durations. Furthermore, different pressure magnitudes were applied to cells. The morphology, cytoskeleton, and expression of myofibroblastic marker protein and transforming growth factor-&beta;1 (TGF-&beta;1) of NRK52E cells were examined. Results: Disorganized actin filaments and formation of curling clusters in actin were seen in the cytoplasm of pressurized cells. We verified that de novo expression of α-smooth muscle actin induced by pressure, which indicated TEMT, was dependent on both the magnitude and duration of pressure. TGF-β1 expression was significantly upregulated under certain conditions, which implies that the induction of TEMT by hydraulic pressure is related with TGF-β1. Conclusion: We illustrate for the first time that hydraulic pressure can induce TEMT in a pressure magnitude- and duration-dependent manner, and that this TEMT is accompanied by TGF-β1 secretion.

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