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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.11 P.885~894


Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction

Author(s):  Xi-sheng XIE, Man YANG, Heng-cuang LIU, Chuan ZUO, Zi LI, Yao DENG, Jun-ming FAN

Affiliation(s):  Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China; more

Corresponding email(s):   junmingfan2007@yahoo.com.cn

Key Words:  Ginsenoside Rg1, Renal fibrosis, Tubular epithelial-myofibroblast transition (TEMT), Thrombospondin-1 (TSP-1), Transforming growth factor-&beta, 1 (TGF-&beta, 1)

Xi-sheng XIE, Man YANG, Heng-cuang LIU, Chuan ZUO, Zi LI, Yao DENG, Jun-ming FAN. Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction[J]. Journal of Zhejiang University Science B, 2008, 9(11): 885~894.

@article{title="Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction",
author="Xi-sheng XIE, Man YANG, Heng-cuang LIU, Chuan ZUO, Zi LI, Yao DENG, Jun-ming FAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction
%A Xi-sheng XIE
%A Heng-cuang LIU
%A Chuan ZUO
%A Zi LI
%A Jun-ming FAN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 11
%P 885~894
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820024

T1 - Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction
A1 - Xi-sheng XIE
A1 - Man YANG
A1 - Heng-cuang LIU
A1 - Chuan ZUO
A1 - Zi LI
A1 - Yao DENG
A1 - Jun-ming FAN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 11
SP - 885
EP - 894
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820024

Total saponins of Panax notoginseng (PNS) have been shown to ameliorate renal interstitial fibrosis. ginsenoside Rg1, a panaxatriol saponin, is one of the major active molecules from PNS. The present study was undertaken to investigate the effect of ginsenoside Rg1 on renal fibrosis in rats with unilateral ureteral obstruction (UUO). The rats were randomly divided into 3 groups: sham-operation (n=15), UUO (n=15) and UUO with ginsenoside Rg1 treatment (n=15, 50 mg per kg body weight, intraperitoneally (i.p.) injected). The rats were sacrificed on Days 7 and 14 after the surgery. Histological examination demonstrated that ginsenoside Rg1 significantly inhibited interstitial fibrosis including tubular injury as well as collagen deposition. α-smooth muscle actin (α-SMA) and E-cadherin are two markers of tubular epithelial-myofibroblast transition (TEMT). Interestingly, ginsenoside Rg1 notably decreased α-SMA expression and simultaneously enhanced E-cadherin expression. The messenger RNA (mRNA) of transforming growth factor-&beta;1 (TGF-&beta;1), a key mediator to regulate TEMT, in the obstructed kidney increased dramatically, but was found to decrease significantly after administration of ginsenoside Rg1. Further study showed that ginsenoside Rg1 considerably decreased the levels of both active TGF-β1 and phosphorylated Smad2 (pSmad2). Moreover, ginsenoside Rg1 substantially suppressed the expression of 1)%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>thrombospondin-1 (TSP-1), a cytokine which can promote the transcription of TGF-β1 mRNA and the activation of latent TGF-β1. These results suggest that ginsenoside Rg1 inhibits renal interstitial fibrosis in rats with UUO. The mechanism might be partly related to the blocking of TEMT via suppressing the expression of TSP-1.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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