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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.11 P.895-902


Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain

Author(s):  Yong-min LIU, Sheng-mei ZHU, Kui-rong WANG, Zhi-ying FENG, Qing-lian CHEN

Affiliation(s):  Department of Anesthesiology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   smzhu20088@yahoo.com.cn

Key Words:  Tramadol, Pain, Mechanical allodynia, Interleukin-6 (IL-6), Interleukin-2 (IL-2), Rat

Yong-min LIU, Sheng-mei ZHU, Kui-rong WANG, Zhi-ying FENG, Qing-lian CHEN. Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain[J]. Journal of Zhejiang University Science B, 2008, 9(11): 895-902.

@article{title="Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain",
author="Yong-min LIU, Sheng-mei ZHU, Kui-rong WANG, Zhi-ying FENG, Qing-lian CHEN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain
%A Yong-min LIU
%A Sheng-mei ZHU
%A Kui-rong WANG
%A Zhi-ying FENG
%A Qing-lian CHEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 11
%P 895-902
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820039

T1 - Effect of tramadol on immune responses and nociceptive thresholds in a rat model of incisional pain
A1 - Yong-min LIU
A1 - Sheng-mei ZHU
A1 - Kui-rong WANG
A1 - Zhi-ying FENG
A1 - Qing-lian CHEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 11
SP - 895
EP - 902
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820039

Objective: To evaluate the effects of tramadol on the proinflammatory responses in a rat model of incisional pain by investigating its effects on nociceptive thresholds and serum interleukin-6 (IL-6) and IL-2 levels. Methods: Forty-two male Sprague-Dawley (SD) rats scheduled for plantar incision were randomly divided into 7 groups (n=6 in each group). rats in Group 1 receiving general anesthesia with no incision were served as control; At 30 min before skin incision, Groups 2~5 were given 5 ml normal saline or 1, 10, and 20 mg/kg tramadol, respectively, intraperitoneally (i.p.); Group 6 received 10 mg/kg tramadol after operation; Group 7 received 10 mg/kg tramadol before incision, followed by 200 μg/kg naloxone after operation. mechanical allodynia was measured by electronic von Frey filament to evaluate the nociceptive thresholds 1 h before incision, and 1 h and 2 h after operation. Serum IL-6 and IL-2 levels were measured by enzyme-linked immunosorbent assay (ELISA) 2 h after operation. Results: Mechanical thresholds decreased significantly and serum IL-6 level increased significantly after operation in Group 2 compared with control (P<0.01), and these changes were reversed respectively by tramadol in a dose-dependent manner (P<0.05 and P<0.01, respectively). IL-2 level remained unchanged after operation in Group 2, but decreased in Group 3 (P<0.05), then gradually returned to the normal level in Groups 4 and 5. The intraperitoneally injected tramadol (10 and 20 mg/kg) produced a potent and dose-dependent antinocicptive effect on the lesioned paw. The antinocicptive effects of tramadol were partially antagonized by naloxone (200 μg/kg), suggesting an additional non-opioid mechanism. Conclusion: The results suggest that tramadol could be a good choice for the treatment of pain under the conditions that immunosuppression may be particularly contraindicated.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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