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Received: 2008-04-06

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.11 P.879~884

http://doi.org/10.1631/jzus.B0820116


Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock


Author(s):  Yuan-qiang LU, Wei-dong HUANG, Xiu-jun CAI, Lin-hui GU, Han-zhou MOU

Affiliation(s):  Department of Emergency, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   luyuanqiang@yahoo.cn, huangweidong2003@hotmail.com

Key Words:  Hemorrhagic shock, Resuscitation, Sodium chloride solution, Hypertonic saline, Apoptosis, Intestinal mucosa, Flow cytometry, In situ nick-end labelling


Yuan-qiang LU, Wei-dong HUANG, Xiu-jun CAI, Lin-hui GU, Han-zhou MOU. Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock[J]. Journal of Zhejiang University Science B, 2008, 9(11): 879~884.

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author="Yuan-qiang LU, Wei-dong HUANG, Xiu-jun CAI, Lin-hui GU, Han-zhou MOU",
journal="Journal of Zhejiang University Science B",
volume="9",
number="11",
pages="879~884",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820116"
}

%0 Journal Article
%T Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock
%A Yuan-qiang LU
%A Wei-dong HUANG
%A Xiu-jun CAI
%A Lin-hui GU
%A Han-zhou MOU
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 11
%P 879~884
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820116

TY - JOUR
T1 - Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock
A1 - Yuan-qiang LU
A1 - Wei-dong HUANG
A1 - Xiu-jun CAI
A1 - Lin-hui GU
A1 - Han-zhou MOU
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 11
SP - 879
EP - 884
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820116


Abstract: 
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mucosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Sprague-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluorescein-iso-thiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P<0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P<0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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