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Received: 2006-03-28

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.70~75


Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock

Author(s):  LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong

Affiliation(s):  Department of Emergency and General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more

Corresponding email(s):   lls201@163.com, luyuanqiang609@yahoo.com.cn

Key Words:  Hemorrhagic shock, Resuscitation, Sodium chloride solution, Hypertonic saline, T-lymphocyte subpopulations, Flow cytometry

LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong. Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock[J]. Journal of Zhejiang University Science B, 2007, 8(1): 70~75.

@article{title="Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock",
author="LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock
%A LU Yuan-qiang
%A CAI Xiu-jun
%A GU Lin-hui
%A MU Han-zhou
%A HUANG Wei-dong
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 1
%P 70~75
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0070

T1 - Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock
A1 - LU Yuan-qiang
A1 - CAI Xiu-jun
A1 - GU Lin-hui
A1 - MU Han-zhou
A1 - HUANG Wei-dong
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 1
SP - 70
EP - 75
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0070

Objective: To investigate the potential and early effect of hypertonic saline resuscitation on t-lymphocyte subpopulations in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley (SD) rats. The rats were randomly divided into Sham group, HTS group (hypertonic saline resuscitation group) and NS group (normal saline resuscitation group). Each group contained 6 rats. The CD4+ and CD8+ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry. Results: In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4+ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8+ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4+/CD8+ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences. Conclusion: In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of t-lymphocyte subpopulations regulating network.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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