Full Text:   <1676>

CLC number: R605.971

On-line Access: 

Received: 2006-03-28

Revision Accepted: 2006-10-10

Crosschecked: 0000-00-00

Cited: 5

Clicked: 3886

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.70~75

http://doi.org/10.1631/jzus.2007.B0070


Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock


Author(s):  LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong

Affiliation(s):  Department of Emergency and General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; more

Corresponding email(s):   lls201@163.com, luyuanqiang609@yahoo.com.cn

Key Words:  Hemorrhagic shock, Resuscitation, Sodium chloride solution, Hypertonic saline, T-lymphocyte subpopulations, Flow cytometry


LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong. Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock[J]. Journal of Zhejiang University Science B, 2007, 8(1): 70~75.

@article{title="Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock",
author="LU Yuan-qiang, CAI Xiu-jun, GU Lin-hui, MU Han-zhou, HUANG Wei-dong",
journal="Journal of Zhejiang University Science B",
volume="8",
number="1",
pages="70~75",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0070"
}

%0 Journal Article
%T Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock
%A LU Yuan-qiang
%A CAI Xiu-jun
%A GU Lin-hui
%A MU Han-zhou
%A HUANG Wei-dong
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 1
%P 70~75
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0070

TY - JOUR
T1 - Hypertonic saline resuscitation maintains a more balanced profile of T-lymphocyte subpopulations in a rat model of hemorrhagic shock
A1 - LU Yuan-qiang
A1 - CAI Xiu-jun
A1 - GU Lin-hui
A1 - MU Han-zhou
A1 - HUANG Wei-dong
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 1
SP - 70
EP - 75
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0070


Abstract: 
Objective: To investigate the potential and early effect of hypertonic saline resuscitation on t-lymphocyte subpopulations in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 18 Sprague-Dawley (SD) rats. The rats were randomly divided into Sham group, HTS group (hypertonic saline resuscitation group) and NS group (normal saline resuscitation group). Each group contained 6 rats. The CD4+ and CD8+ subpopulations of T-lymphocytes in peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry. Results: In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the CD4+ lymphocytes of peripheral blood in HTS and NS groups markedly increased. Small volume resuscitation with HTS also induced peripheral CD8+ lymphocytes to a certain extent, whereas NS resuscitation showed no effect in this respect. Consequently, compared with Sham and HTS groups, CD4+/CD8+ ratio of peripheral blood in NS group was obviously increased, and showed statistically differences. Conclusion: In this model of rat with severe hemorrhagic shock, small volume resuscitation with HTS is more effective than NS in reducing immunologic disorders and promoting a more balanced profile of t-lymphocyte subpopulations regulating network.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Bahrami, S., Zimmermann, K., Szelenyi, Z., Hamar, J., Scheiflinger, F., Redl, H., Junger, W.G., 2006. Small-volume fluid resuscitation with hypertonic saline prevents inflammation but not mortality in a rat model of hemorrhagic shock. Shock, 25(3):283-289.

[2] Bellamy, R.F., 1984. The causes of death in conventional land warfare: implications for combat casualty care research. Mil. Med., 149(2):55-62.

[3] Cai, X.J., Huang, D.Y., Mu, Y.P., Peng, S.Y., 2002. Hypertonic saline solution resuscitation in hemorrhagic shock dog. Chin. J. Traumatol., 5(3):180-185.

[4] Capone, A.C., Safar, P., Stezoski, W., Tisherman, S., Peitzman, A.B., 1995. Improved outcome with fluid restriction in treatment of uncontrolled hemorrhagic shock. J. Am. Coll. Surg., 180(1):49-56.

[5] Cheadle, W.G., Pemberton, R.M., Robinson, D., Livingston, D.H., Rodriguez, J.L., Polk, H.C.Jr, 1993. Lymphocyte subset responses to trauma and sepsis. J. Trauma., 35(6):844-849.

[6] Coimbra, R., Loomis, W., Melbostad, H., Tobar, M., Porcides, R.D., Lall, R., Holbrook, T., Hoyt, D.B., 2005. Role of hypertonic saline and pentoxifylline on neutrophil activation and tumor necrosis factor-alpha synthesis: a novel resuscitation strategy. J. Trauma., 59(2):257-264.

[7] Cross, J.S., Gruber, D.P., Gann, D.S., Singh, A.K., Moran, J.M., Burchard, K.W., 1989. Hypertonic saline attenuates the hormonal response to injury. Ann. Surg., 209(6):684-692.

[8] Cruz, R.J.Jr, Yada-Langui, M.M., de Figueiredo, L.F., Sinosaki, S., Rocha-e-Silva, M., 2006. The synergistic effects of pentoxifylline on systemic and regional perfusion after hemorrhage and hypertonic resuscitation. Anesth. Analg., 102(5):1518-1524.

[9] Deitch, E.A., Shi, H.P., Feketeova, E., Hauser, C.J., Xu, D.Z., 2003. Hypertonic saline resuscitation limits neutrophil activation after trauma-hemorrhagic shock. Shock, 19(4):328-333.

[10] Giannoudis, P.V., 2003. Current concepts of the inflammatory response after major trauma:an update. Injury, 34(6):397-404.

[11] Homma, H., Deitch, E.A., Feketeova, E., Lu, Q., Berezina, T.L., Zaets, S.B., Machiedo, G.W., Xu, D.Z., 2005. Small volume resuscitation with hypertonic saline is more effective in ameliorating trauma-hemorrhagic shock-induced lung injury, neutrophil activation and red blood cell dysfunction than pancreatitic protease inhibition. J. Trauma., 59(2):266-272.

[12] Hoppen, R.A., Corso, C.O., Grezzana, T.J., Severino, A., Dal-Pizzol, F., Ritter, C., 2005. Hypertonic saline and hemorrhagic shock: hepatocellular function and integrity after six hours of treatment. Acta Cir. Bras., 20(6):414-417.

[13] Hoyt, D.B., 2004. A clinical review of bleeding dilemmas in trauma. Semin. Hematol., 41(1 Suppl. 1):40-43.

[14] Junger, W.G., Liu, F.C., Loomis, W.H., Hoyt, D.B., 1994. Hypertonic saline enhances cellular immune function. Circ. Shock, 42(4):190-196.

[15] Junger, W.G., Coimbra, R., Liu, F.C., Herdon-Remelius, C., Junger, W., Junger, H., Loomis, W., Hoyt, D.B., Altman, A., 1997a. Hypertonic saline resuscitation: a tool to modulate immune function in trauma patients? Shock, 8(4):235-241.

[16] Junger, W.G., Hoyt, D.B., Hamreus, M., Liu, F.C., Herdon-Remelius, C., Junger, W., Altman, A., 1997b. Hypertonic saline activates protein tyrosine kinases and mitogen-activated protein kinase p38 in T-cells. J. Trauma., 42(3):437-443.

[17] Junger, W.G., Hoyt, D.B., Davis, R.E., Herdon-Remelius, C., Namiki, S., Junger, H., Loomis, W., Altman, A., 1998. Hypertonicity regulates the function of human neutrophils by modulating chemoattractant receptor signaling and activating mitogen-activated protein kinase p38. J. Clin. Invest., 101(12):2768-2779.

[18] Kolsen-Petersen, J.A., 2004. Immune effect of hypertonic saline: fact or fiction? Acta Anaesthesiol. Scand., 48(6):667-678.

[19] Loomis, W.H., Namiki, S., Hoyt, D.B., Junger, W.G., 2001. Hypertonicity rescues T cells from suppression by trauma-induced anti-inflammatory mediators. Am. J. Physiol. Cell Physiol., 281(3):C840-C848.

[20] Lu, Y.Q., Cai, X.J., Gu, L.H., Fan, Y.J., Wang, Q., Bao, D.G., 2005. Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock. J. Zhejiang Univ. Sci. B, 6(9):907-912.

[21] Lu, Y.Q., Cai, X.J., Gu, L.H., Wang, Q., Huang, W.D., Bao, D.G., 2006. Early difference in apoptosis of intestinal mucosa of rats with severe uncontrolled hemorrhagic shock after three fluid resuscitation methods. Chin. Med. J., 119(10):858-863.

[22] Maier, R.V., 2000. Pathogenesis of multiple organ dysfunction syndrome—endotoxin, inflammatory cells, and their mediators: cytokines and reactive oxygen species. Surg. Infect. (Larchmt), 1(3):197-205.

[23] Marshall, J.C., Cohen, J., 2000. Immune Response in the Critically Ill. Springer-Verlag, New York.

[24] Moore, F.A., McKinley, B.A., Moore, E.E., 2004. The next generation in shock resuscitation. Lancet, 363(9425):1988-1996.

[25] Oberholzer, A., Oberholzer, C., Moldawer, L.L., 2000. Cytokine signaling—regulation of the immune response in normal and critically ill states. Crit. Care Med., 28(4 Suppl):N3-N12.

[26] Ozguc, H., Tokyay, R., Kahveci, N., Serdar, Z., Gur, E.S., 2003. Hypertonic saline dextran alleviates hepatic injury in hypovolemic rats undergoing porta hepatis occlusion. Shock, 19(4):383-387.

[27] Powers, K.A., Woo, J., Khadaroo, R.G., Papia, G., Kapus, A., Rotstein, O.D., 2003. Hypertonic resuscitation of hemorrhagic shock upregulates the anti-inflammatory response by alveolar macrophages. Surgery, 134(2):312-318.

[28] Rhee, P., Wang, D., Ruff, P., Austin, B., DeBraux, S., Wolcott, K., Burris, D., Ling, G., Sun, L., 2000. Human neutrophil activation and increased adhesion by various resuscitation fluids. Crit. Care Med., 28(1):74-78.

[29] Rizoli, S.B., Kapus, A., Fan, J., Li, Y.H., Marshall, J.C., Rotstein, O.D., 1998. Immunomodulatory effects of hypertonic resuscitation on the development of lung inflammation following hemorrhagic shock. J. Immunol., 161(11):6288-6296.

[30] Rizoli, S.B., Rhind, S.G., Shek, P.N., Inaba, K., Filips, D., Tien, H., Brenneman, F., Rotstein, O.D., 2006. The immuno-modulatory effects of hypertonic saline resuscitation in patients sustaining traumatic hemorrhagic shock: a randomized, controlled, double-blinded trial. Ann. Surg., 243(1):47-57.

[31] Rocha-e-Silva, M., Negraes, G.A., Soares, A.M., Pontieri, V., Loppnow, L., 1986. Hypertonic resuscitation from severe hemorrhagic shock: pattern of regional circulation. Circ. Shock, 19(2):165-175.

[32] Rotstein, O.D., 2000. Novel strategies for immunomodulation after trauma: revisiting hypertonic saline as a resuscitation strategy for hemorrhagic shock. J. Trauma., 49(4):580-583.

[33] Rotstein, O.D., 2003. Modeling the two-hit hypothesis for evaluating strategies to prevent organ injury after shock/resuscitation. J. Trauma., 54(Suppl):203-206.

[34] Shields, C.J., Winter, D.C., Manning, B.J., Wang, J.H., Kirwan, W.O., Redmond, H.P., 2003. Hypertonic saline infusion for pulmonary injury due to ischemia-reperfusion. Arch. Surg., 138(1):9-14.

[35] Victorino, G.P., Newton, C.R., Curran, B., 2003. Effect of hypertonic saline on microvascular permeability in the activated endothelium. J. Surg. Res., 112(1):79-83.

[36] Wade, C.E., Hannon, J.P., Bossone, C.A., Hunt, M.M., Loveday, J.A., Coppes, R.I.Jr, Gildengorin, V.L., 1991. Neuroendocrine responses to hypertonic saline/dextran resuscitation following hemorrhage. Circ. Shock, 35(1):37-43.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE