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Received: 2008-08-22

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.12 P.923~930

10.1631/jzus.B0820257


Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels


Author(s):  Shang-zhe XIE, Ning-tao FANG, Shui LIU, Ping ZHOU, Yi ZHANG, Song-mei WANG, Hong-yang GAO, Luan-feng PAN

Affiliation(s):  Laboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China; more

Corresponding email(s):   lfpan@shmu.edu.cn

Key Words:  Smooth muscle progenitor cells (SPCs), Tissue-engineered blood vessels (TEBVs), Silk fibroin (SF), Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx)


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Shang-zhe XIE, Ning-tao FANG, Shui LIU, Ping ZHOU, Yi ZHANG, Song-mei WANG, Hong-yang GAO, Luan-feng PAN. Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels[J]. Journal of Zhejiang University Science B, 2008, 9(12): 923~930.

@article{title="Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels",
author="Shang-zhe XIE, Ning-tao FANG, Shui LIU, Ping ZHOU, Yi ZHANG, Song-mei WANG, Hong-yang GAO, Luan-feng PAN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="12",
pages="923~930",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820257"
}

%0 Journal Article
%T Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels
%A Shang-zhe XIE
%A Ning-tao FANG
%A Shui LIU
%A Ping ZHOU
%A Yi ZHANG
%A Song-mei WANG
%A Hong-yang GAO
%A Luan-feng PAN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 12
%P 923~930
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820257

TY - JOUR
T1 - Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels
A1 - Shang-zhe XIE
A1 - Ning-tao FANG
A1 - Shui LIU
A1 - Ping ZHOU
A1 - Yi ZHANG
A1 - Song-mei WANG
A1 - Hong-yang GAO
A1 - Luan-feng PAN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 12
SP - 923
EP - 930
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820257


Abstract: 
Background: A major shortcoming in tissue engineered blood vessels (TEBVs) is the lack of healthy and easily attainable smooth muscle cells (SMCs). smooth muscle progenitor cells (SPCs), especially from peripheral blood, may offer an alternative cell source for tissue engineering involving a less invasive harvesting technique. Methods: SPCs were isolated from 5-ml fresh rat peripheral blood by density-gradient centrifugation and cultured for 3 weeks in endothelial growth medium-2-MV (EGM-2-MV) medium containing platelet-derived growth factor-BB (PDGF BB). Before seeded on the synthesized scaffold, SPC-derived smooth muscle outgrowth cell (SOC) phenotypes were assessed by immuno-fluorescent staining, Western blot analysis, and reverse transcription polymerase chain reaction (RT-PCR). The cells were seeded onto the silk fibroin-modified poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (SF-PHBHHx) scaffolds by 6×104 cells/cm2 and cultured under the static condition for 3 weeks. The growth and proliferation of the seeded cells on the scaffold were analyzed by 3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide (MTT) assay, scanning electron microscope (SEM), and 4,6-diamidino-2-phenylindole (DAPI) staining. Results: SOCs displayed specific “hill and valley” morphology, expressed the specific markers of the SMC lineage: smooth muscle (SM) α-actin, calponin and smooth muscle myosin heavy chain (SM MHC) at protein and messenger ribonucleic acid (mRNA) levels. RT-PCR results demonstrate that SOCs also expressed smooth muscle protein 22α (SM22α), a contractile protein, and extracellular matrix components elastin and matrix Gla protein (MGP), as well as vascular endothelial growth factor (VEGF). After seeded on the SF-PHBHHx scaffold, the cells showed excellent metabolic activity and proliferation. Conclusion: SPCs isolated from peripheral blood can be differentiated into the SMCs in vitro and have an impressive growth potential in the biodegradable synthesized scaffold. Thus, SPCs may be a promising cell source for constructing TEBVs.

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