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CLC number: S158.5

On-line Access: 2016-04-05

Received: 2015-08-28

Revision Accepted: 2015-12-08

Crosschecked: 2016-03-18

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Xiao-chuang Cao


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.4 P.294-302


Effects of ammonium application rate on uptake of soil adsorbed amino acids by rice

Author(s):  Xiao-chuang Cao, Qing-xu Ma, Liang-huan Wu, Lian-feng Zhu, Qian-yu Jin

Affiliation(s):  State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; more

Corresponding email(s):   caoxiaochuang@126.com, finm@zju.edu.cn

Key Words:  Soil adsorbed glycine, Ammonium, Glycine uptake, Glycine bioavailability, Sterile cultivation

Xiao-chuang Cao, Qing-xu Ma, Liang-huan Wu, Lian-feng Zhu, Qian-yu Jin. Effects of ammonium application rate on uptake of soil adsorbed amino acids by rice[J]. Journal of Zhejiang University Science B, 2016, 17(4): 294-302.

@article{title="Effects of ammonium application rate on uptake of soil adsorbed amino acids by rice",
author="Xiao-chuang Cao, Qing-xu Ma, Liang-huan Wu, Lian-feng Zhu, Qian-yu Jin",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of ammonium application rate on uptake of soil adsorbed amino acids by rice
%A Xiao-chuang Cao
%A Qing-xu Ma
%A Liang-huan Wu
%A Lian-feng Zhu
%A Qian-yu Jin
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 4
%P 294-302
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500203

T1 - Effects of ammonium application rate on uptake of soil adsorbed amino acids by rice
A1 - Xiao-chuang Cao
A1 - Qing-xu Ma
A1 - Liang-huan Wu
A1 - Lian-feng Zhu
A1 - Qian-yu Jin
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 4
SP - 294
EP - 302
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500203

In recent years, excessive use of chemical nitrogen (N) fertilizers has resulted in the accumulation of excess ammonium (NH4+) in many agricultural soils. Though rice is known as an NH4+-tolerant species and can directly absorb soil intact amino acids, we still know considerably less about the role of high exogenous NH4+ content on rice uptake of soil amino acids. This experiment examined the effects of the exogenous NH4+ concentration on rice uptake of soil adsorbed glycine in two different soils under sterile culture. Our data showed that the sorption capacity of glycine was closely related to soils’ physical and chemical properties, such as organic matter and cation exchange capacity. Rice biomass was significantly inhibited by the exogenous NH4+ content at different glycine adsorption concentrations. A three-way analysis of variance demonstrated that rice glycine uptake and glycine nutritional contribution were not related to its sorption capacity, but significantly related to its glycine:NH4+ concentration ratio. After 21-d sterile cultivation, the rice uptake of adsorbed glycine accounted for 8.8%‒22.6% of rice total N uptake, which indicates that soil adsorbed amino acids theoretically can serve as an important N source for plant growth in spite of a high NH4+ application rate. However, further studies are needed to investigate the extent to which this bioavailability is realized in the field using the 13C, 15N double labeling technology.


方法:采集两种不同生态系统的土壤A和B,经0.5 mol/L K2SO4连续淋洗5次,121 °C灭菌30 min,15N-甘氨酸处理后,根据甘氨酸吸附曲线(图1)确定甘氨酸吸附饱和点和吸附半饱和点,然后向土壤中添加一些不同浓度的铵态氮,水稻幼苗无菌培养21天后,用MAT-271质谱仪测定水稻幼苗氨基酸吸收量。
结论:实验结果表明土壤甘氨酸吸附能力大小与土壤理化性质紧密相关, 如有机质和阳离子交换量。外源高铵态氮水平显著抑制水稻幼苗生长发育 (P<0.05),但甘氨酸吸收及其氮营养贡献与甘氨酸吸附能力大小无关,而与土壤吸附态甘氨酸和铵态氮的浓度比值显著相关(P<0.05)。经过21天的无菌培养,土壤吸附态氨基酸对水稻的氮营养贡献率达8.8%~22.6%,表明土壤吸附态氨基酸理论上可能作为植物的一种潜在重要营养氮源。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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