Full Text:   <2428>

Summary:  <1738>

CLC number: TS252.7

On-line Access: 2015-08-04

Received: 2014-12-20

Revision Accepted: 2015-05-11

Crosschecked: 2015-07-09

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Zhan-mei Jiang


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.8 P.672-679


Development of a test strip for rapid detection of lactoperoxidase in raw milk

Author(s):  Hong-xia Che, Bo Tian, Li-na Bai, Li-ming Cheng, Li-li Liu, Xiao-na Zhang, Zhan-mei Jiang, Xiao-xi Xu

Affiliation(s):  Key Laboratory of Dairy Science (KLDS), Ministry of Education, Northeast Agricultural University, Harbin 150030, China

Corresponding email(s):   zhanmeijiang@neau.edu.cn, xiaoxi_xu01@163.com

Key Words:  Lactoperoxidase, Test strip, Rapid detection, Raw milk, 3, 3'5, 5'-Tetramethylbenzidine (TMB)

Hong-xia Che, Bo Tian, Li-na Bai, Li-ming Cheng, Li-li Liu, Xiao-na Zhang, Zhan-mei Jiang, Xiao-xi Xu. Development of a test strip for rapid detection of lactoperoxidase in raw milk[J]. Journal of Zhejiang University Science B, 2015, 16(8): 672-679.

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author="Hong-xia Che, Bo Tian, Li-na Bai, Li-ming Cheng, Li-li Liu, Xiao-na Zhang, Zhan-mei Jiang, Xiao-xi Xu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Development of a test strip for rapid detection of lactoperoxidase in raw milk
%A Hong-xia Che
%A Bo Tian
%A Li-na Bai
%A Li-ming Cheng
%A Li-li Liu
%A Xiao-na Zhang
%A Zhan-mei Jiang
%A Xiao-xi Xu
%J Journal of Zhejiang University SCIENCE B
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%P 672-679
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400359

T1 - Development of a test strip for rapid detection of lactoperoxidase in raw milk
A1 - Hong-xia Che
A1 - Bo Tian
A1 - Li-na Bai
A1 - Li-ming Cheng
A1 - Li-li Liu
A1 - Xiao-na Zhang
A1 - Zhan-mei Jiang
A1 - Xiao-xi Xu
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 8
SP - 672
EP - 679
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400359

Traditional methods for detecting lactoperoxidase (LP) are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid (CA)/0.2 mol/L disodium hydrogen phosphate (NaP) buffer solution (pH 5.0), 22 mmol/L 3,3',5,5'-Tetramethylbenzidine (TMB), 0.6 mmol/L hydrogen peroxide (H2O2), and 0.5% Tween-20 or 0.3% cetyltrimethyl ammonium bromide (CTAB) were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation (CV) of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1ȁ3;2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation (r=0.9776). So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.


结论:试纸检测体系的优化结果为0.1 mol/L柠檬酸/0.2 mol/L磷酸氢二钠溶液(pH 5.0),22 mmol/L TMB,0.5% Tween-20或0.3% 溴化十六烷基三甲铵(CTAB),和0.6 mmol/L H2O2。检测试纸的精密度较好,变异系数在 2.47%~6.72%(表1);最低检出浓度为1.000~2.000 mg/L(图7);与试管法检测结果的相关系数为0.9776(图8)。因此,试纸检测法是一种快速、灵敏和准确的乳过氧化物酶检测技术,可满足原料乳验收及相关乳制品加工中对乳过氧化物酶检测的需求。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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