Journal of Zhejiang University

Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.11 P.1006-1010

A mutation of Aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor*

Author(s): Wei Hu^1,2,3,Jing Liu^1,3,Ji-hong Chen^1,3,Shu-yang Wang^1,3,Dong Lu^1,3,Qing-hua Wu^1,3,Wen-jian Li^1,3
Affiliation(s): 1. 1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China more
Corresponding email(s): chjh@impcas.ac.cn
Key Words: Mutation, Citric acid, Corn meal hydrolysate, Aspergillus niger

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Wei Hu, Jing Liu, Ji-hong Chen, Shu-yang Wang, Dong Lu, Qing-hua Wu, Wen-jian Li. A mutation of Aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor[J]. Journal of Zhejiang University Science B, 2014, 15(11): 1006-1010.

@article{title="A mutation of Aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor",
author="Wei Hu, Jing Liu, Ji-hong Chen, Shu-yang Wang, Dong Lu, Qing-hua Wu, Wen-jian Li",
journal="Journal of Zhejiang University Science B",
volume="15",
number="11",
pages="1006-1010",
year="2014",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400132"
}

%0 Journal Article
%T A mutation of Aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor
%A Wei Hu
%A Jing Liu
%A Ji-hong Chen
%A Shu-yang Wang
%A Dong Lu
%A Qing-hua Wu
%A Wen-jian Li
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 11
%P 1006-1010
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400132

TY - JOUR
T1 - A mutation of Aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor
A1 - Wei Hu
A1 - Jing Liu
A1 - Ji-hong Chen
A1 - Shu-yang Wang
A1 - Dong Lu
A1 - Qing-hua Wu
A1 - Wen-jian Li
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 11
SP - 1006
EP - 1010
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400132

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: The properties of the screened mutants for hyper-production of citric acid induced by carbon (¹²C⁶⁺) ion beams and X-ray irradiation were investigated in our current study. Among these mutants, mutant H4002 screened from ¹²C⁶⁺ ion irradiation had a higher yield of citric acid production than the parental strain in a 250-ml shaking flash. These expanded submerged experiments in a bioreactor were also carried out for mutant H4002. The results showed that (177.7–196.0) g/L citric acid was accumulated by H4002 through exploiting corn meal hydrolysate (containing initial 200.0–235.7 g/L sugar) with the productivity of (2.96–3.27) g/(L∙h). This was especially true when the initial sugar concentration was 210 g/L, and the best economical citric acid production reached (187.5±0.7) g/L with a productivity of 3.13 g/(L∙h). It was observed that mutant H4002 can utilize low-cost corn meal as a feedstock to efficiently produce citric acid. These results imply that the H4002 strain has the industrial production potentiality for citric acid and offers strong competition for the citric acid industry.

高产柠檬酸突变菌株以玉米粉为原料的生物反应器扩大生产研究

研究高产黑曲霉突变菌株以玉米粉为原料的生物反应器扩大发酵，以期获得适合于工业化生产柠檬酸的发酵工艺。以玉米粉为原料，系统地研究了筛选得到的高产菌株在50 L生物反应器中不同糖浓度发酵生产柠檬酸的特性，最终优化出适合于工业化生产柠檬酸的发酵工艺。（1）利用淀粉酶对粉碎后的玉米进行液化，然后过滤，最终得上清液；（2）以50 L生物反应器作为发酵设备，对筛选得到的高产柠檬酸菌株进行扩大培养；（3）通过测定不同培养时期中积累的柠檬酸含量和剩余的残总糖，最终优化出高效率生产柠檬酸的发酵工艺。以不同糖浓度的液化玉米粉上清液作为碳源，突变菌株H4002能积累177.7~196.0 g/L的柠檬酸，效率能达到2.96~3.27 g/(L∙h)，尤其当糖浓度为210 g/L，H4002菌株表现出最佳的柠檬酸生产水平，如柠檬酸积累187.5 g/L，生产效率达3.13 g/(L∙h)。上述结果说明了突变菌株H4002拥有快速生产柠檬酸的能力。
突变；柠檬酸生产；玉米粉液化液；黑曲霉