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On-line Access: 2019-07-16
Received: 2019-06-15
Revision Accepted: 2019-07-04
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Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height
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Maha Alruwaili, Jose A. Lopez, Kevin McCarthy, Emmanuel G, Reynaud, Brian J. Rodriguez. Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height[J]. Journal of Zhejiang University Science D, 2019, 2(3): 172-180.
@article{title="Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height",
author="Maha Alruwaili, Jose A. Lopez, Kevin McCarthy, Emmanuel G, Reynaud, Brian J. Rodriguez",
journal="Journal of Zhejiang University Science D",
volume="2",
number="3",
pages="172-180",
year="2019",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-019-00043-w"
}
%0 Journal Article
%T Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height
%A Maha Alruwaili
%A Jose A. Lopez
%A Kevin McCarthy
%A Emmanuel G
%A Reynaud
%A Brian J. Rodriguez
%J Journal of Zhejiang University SCIENCE D
%V 2
%N 3
%P 172-180
%@ 1869-1951
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-019-00043-w
TY - JOUR
T1 - Liquid-phase 3D bioprinting of gelatin alginate hydrogels: influence of printing parameters on hydrogel line width and layer height
A1 - Maha Alruwaili
A1 - Jose A. Lopez
A1 - Kevin McCarthy
A1 - Emmanuel G
A1 - Reynaud
A1 - Brian J. Rodriguez
J0 - Journal of Zhejiang University Science D
VL - 2
IS - 3
SP - 172
EP - 180
%@ 1869-1951
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-019-00043-w
Abstract: extrusion-based 3D bioprinting is a direct deposition approach used to create three-dimensional (3D) tissue scaffolds typically comprising hydrogels. hydrogels are hydrated polymer networks that are chemically or physically cross-linked. Often, 3D bioprinting is performed in air, despite the hydrated nature of hydrogels and the potential advantage of using a liquid phase to provide cross-linking and otherwise functionalize the hydrogel. In this work, we print gelatin alginate hydrogels directly into a cross-linking solution of calcium chloride and investigate the influence of nozzle diameter, distance between nozzle and surface, calcium chloride concentration, and extrusion rate on the dimensions of the printed hydrogel. The hydrogel layer height was generally found to increase with increasing extrusion rate and nozzle distance, according to the increased volume extruded and the available space, respectively. In addition, the hydrogel width was generally found to increase with decreasing nozzle distance and cross-linking concentration corresponding to confinement-induced spreading and low cross-linking regimes, respectively. Width/height ratios of ~ 1 were generally achieved when the nozzle diameter and distance were comparable above a certain cross-linking concentration. Using these relationships, biocompatible 3D multilayer structures were successfully printed directly into calcium chloride cross-linking solution.
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