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Bio-Design and Manufacturing  2023 Vol.6 No.4 P.439-450

http://doi.org/10.1007/s42242-023-00238-2


3D-printed engineered bacteria-laden gelatin/sodium alginate composite hydrogels for biological detection of ionizing radiation


Author(s):  Ziyuan Chen, Jintao Shen, Meng Wei, Wenrui Yan, Qiucheng Yan, Zhangyu Li, Yaqiong Chen, Feng Zhang, Lina Du, Bochuan Yuan & Yiguang Jin

Affiliation(s):  Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing 100850, China

Corresponding email(s):   bochuanyuan@sina.com, jinyg@sina.com

Key Words:  3D printing, Alginate, Engineered bacteria, Gelatin, Hydrogel, Ionizing radiation


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Ziyuan Chen, Jintao Shen, Meng Wei, Wenrui Yan, Qiucheng Yan, Zhangyu Li, Yaqiong Chen, Feng Zhang, Lina Du, Bochuan Yuan & Yiguang Jin. 3D-printed engineered bacteria-laden gelatin/sodium alginate composite hydrogels for biological detection of ionizing radiation[J]. Journal of Zhejiang University Science D, 2023, 6(4): 439-450.

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Abstract: 
Nuclear safety is a global growing concern, where ionizing radiation (IR) is a major injury factor resulting in serious damage to organisms. The detection of IR is usually conducted with physical dosimeters; however, biological IR detection methods are deficient. Here, a living composite hydrogel consisting of engineered bacteria and gelatin/sodium alginate was 3D-printed for the biological detection of IR. Three strains of PrecA::egfp gene circuit-containing engineered Escherichia coli were constructed with IR-dependent fluorescence, and the DH5α strain was finally selected due to its highest radiation response and fluorescence. engineered bacteria were loaded in a series of gelatin/sodium alginate matrix hydrogels with different rheology, 3D printability and bacterial applicability. A high-gelatin-content hydrogel containing 10% gelatin/1.25% sodium alginate was optimal. The optimal living composite hydrogel was 3D-printed with the special bioink, which reported significant green fluorescence under γ -ray radiation. The living composite hydrogel provides a biological strategy for the detection of environmental ionizing radiation.

军事科学院军事医学研究院金义光团队 | 3D打印装载工程菌的明胶海藻酸钠复合水凝胶用于电离辐射的生物检测

本研究论文聚焦于工程化细菌检测环境因素的载体制备及制造研究。利用工程菌检测环境中的化学毒物、重金属、放射性物质等有害因素具有广阔的应用前景,但由于工程菌自身条件限制,大多无法在外界环境中现场应用。近年来,生物3D打印水凝胶被广泛用于活细胞培养、组织工程等方面,其在保证细胞活性的前提下还可根据不同应用场景进行形状的个性化设计,有望解决工程菌现场应用问题。本研究从环境电离辐射检测的问题出发,通过构建工程菌并根据性能进行筛选,得到了可在电离辐射诱导下产生绿色荧光信号的辐射响应工程菌。以明胶和海藻酸钠作为主要材料,设计不同配方的水凝胶,通过比较其成胶性能、压缩性能和可打印性等筛选得到最优的水凝胶载体。将工程菌载入水凝胶载体中,通过存活率和荧光强度检测发现3D打印水凝胶可作为活细菌的优良载体,为工程菌提供与培养基相媲美的培养条件以及支撑和保护作用。最终制备得到的3D打印载菌明胶/海藻酸钠复合水凝胶能在受到电离辐射时产生绿色荧光,实现环境电离辐射的报告。本研究采用的生物3D打印水凝胶技术为工程菌实现环境监测应用提供了新的策略,为环境电离辐射监测提供了一种新的方法。

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