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Bio-Design and Manufacturing  2023 Vol.6 No.2 P.150-173

http://doi.org/10.1007/s42242-022-00194-3


Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-based multi-material hydrogel composites


Author(s):  Suihong Liu, Haiguang Zhang, Tilman Ahlfeld, David Kilian, Yakui Liu, Michael Gelinsky & Qingxi Hu

Affiliation(s):  Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany

Corresponding email(s):   michael.gelinsky@tu-dresden.de, huqingxi@shu.edu.cn

Key Words:  Multi-material composite hydrogel, Crosslinking mechanism, Chitosan, Gelatin, Egg white, 3D printing


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Suihong Liu, Haiguang Zhang, Tilman Ahlfeld, David Kilian, Yakui Liu, Michael Gelinsky & Qingxi Hu. Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-based multi-material hydrogel composites[J]. Journal of Zhejiang University Science D, 2023, 6(2): 150-173.

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Abstract: 
Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to the precise spatial control over depositing the biomaterial. Despite their widespread utilization and numerous advantages, the development of suitable novel biomaterials for extrusion-based 3D printing of scaffolds that support cell attachment, proliferation, and vascularization remains a challenge. multi-material composite hydrogels present incredible potential in this field. Thus, in this work, a multi-material composite hydrogel with a promising formulation of chitosan/gelatin functionalized with egg white was developed, which provides good printability and shape fidelity. In addition, a series of comparative analyses of different crosslinking agents and processes based on tripolyphosphate (TPP), genipin (GP), and glutaraldehyde (GTA) were investigated and compared to select the ideal crosslinking strategy to enhance the physicochemical and biological properties of the fabricated scaffolds. All of the results indicate that the composite hydrogel and the resulting scaffolds utilizing TPP crosslinking have great potential in tissue engineering, especially for supporting neo-vessel growth into the scaffold and promoting angiogenesis within engineered tissues.

TU Dresden和上大刘随红等 | 评估不同交联策略对3D打印壳聚糖基水凝胶复合材料性能的影响

本研究论文聚焦不同交联策略对挤出3D打印壳聚糖基多材料水凝胶复合材料性能的影响评估。3D打印技术(特别是基于挤出方式的3D打印技术)可对生物材料墨水进行空间范围的精确控制和堆积塑形,在组织工程和再生医学的发展领域显示出巨大的潜力。尽管该3D打印技术具有独特优势且被广泛应用于生物制造领域,但研制适用于挤出3D打印且能够促进细胞黏附、生长和血管化的生物材料墨水以及合适的交联策略一直是该领域的一大挑战。因此,本研究论文研发了一种基于壳聚糖/明胶和蛋清蛋白复合的功能化水凝胶生物墨水,其具有良好的可打印性及形状保真度。此外,为了选择合适的交联剂和方法,对三聚磷酸盐 (Tripolyphosphate, TPP)、京尼平 (Genipin, GP)和戊二醛 (Glutaraldehyde, GTA)三种交联剂和五种组合交联方法进行一系列理化及生物学性能对比和研究分析,研究结果发现基于TPP交联的支架在增强理化性能的同时保持了良好的生物学性能且具有较高的应用性价比。此外,打印制备的复合支架的功能性研究表明该蛋白化的水凝胶支架有助于宿主新生血管长入支架,能够促进与宿主间的融合及组织工程支架的血管化,在组织工程和再生医学领域具有广阔的应用前景。
(文章作者还包括上海大学张海光教授和硕士研究生刘亚魁,TU Dresden的David Kilian和Ahlfeld Tilman博士;TU Dresden的Michael Gelinsky教授和上海大学胡庆夕教授为通讯作者)

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