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On-line Access: 2022-09-30

Received: 2022-04-28

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Bio-Design and Manufacturing  2023 Vol.6 No.1 P.90-97

http://doi.org/10.1007/s42242-022-00211-5


High-efficiency 3D cell spheroid formation via the inertial focusing effect in rotating droplets


Author(s):  Tan Tang, Peipei Zhang, Yanran Wei, Hongxing Jia, Lin Feng & Ye Xu

Affiliation(s):  School of Mechanical Engineering and Automation, Beihang University, Beijing 100191, China; more

Corresponding email(s):   ye.xu@buaa.edu.cn

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Tan Tang, Peipei Zhang, Yanran Wei, Hongxing Jia, Lin Feng & Ye Xu. High-efficiency 3D cell spheroid formation via the inertial focusing effect in rotating droplets[J]. Journal of Zhejiang University Science D, 2023, 6(1): 90-97.

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Abstract: 

北航徐晔等 | 基于旋转悬滴中惯性流的粒子聚集效应高效制备三维细胞模型

本Technical Note聚焦利用旋转悬滴中惯性流的粒子聚焦效应快速制备三维细胞模型的方法。三维细胞培养技术是体外生物研究的重要工具,近年来得到不断发展。但是现有三维细胞培养技术仍然存在制备时间长、模型均一性差、尺寸不可控的问题。在本次研究中,我们利用惯性流的粒子聚焦效应开发了一种简单又高效的三维细胞模型制备方法。这种方法可以将三维细胞模型的制备时间缩短到12小时,与传统方法相比明显提升了制备效率。由于惯性流的粒子聚焦效应,悬液中的细胞首先被快速聚焦到底部,极大地提高了在后续培养中形成单一细胞球的效率。我们可以改变悬液中的细胞数量,来调控所制备细胞模型的尺寸。另外,改变悬滴的形状可以制备出不同形状的三维细胞模型。利用这种方法,我们开发了三种细胞共培养模型来模拟体内细胞与外环境相互作用的不同状态。为了验证方法的实用性,我们使用该方法制备三维肿瘤球进行抗癌药物检测和毛囊干细胞球进行干细胞分化实验。本文介绍的方法有望在药物筛选、组织工程和生物医学研究中发挥重要作用。

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