CLC number:
On-line Access: 2024-03-26
Received: 2023-08-10
Revision Accepted: 2023-12-24
Crosschecked: 0000-00-00
Cited: 0
Clicked: 305
Ding Wang, Qinyu Li, Chenyang Zhou, Zhangjie Li, Kangyi Lu, Yijun Liu, Lian Xuan & Xiaolin Wang. Dissolvable temporary barrier: a novel paradigm for flexible hydrogel patterning in organ-on-a-chip models[J]. Journal of Zhejiang University Science D, 2024, 7(2): 153-166.
@article{title="Dissolvable temporary barrier: a novel paradigm for flexible hydrogel
patterning in organ-on-a-chip models",
author="Ding Wang, Qinyu Li, Chenyang Zhou, Zhangjie Li, Kangyi Lu, Yijun Liu, Lian Xuan & Xiaolin Wang",
journal="Journal of Zhejiang University Science D",
volume="7",
number="2",
pages="153-166",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-023-00267-x"
}
%0 Journal Article
%T Dissolvable temporary barrier: a novel paradigm for flexible hydrogel
patterning in organ-on-a-chip models
%A Ding Wang
%A Qinyu Li
%A Chenyang Zhou
%A Zhangjie Li
%A Kangyi Lu
%A Yijun Liu
%A Lian Xuan & Xiaolin Wang
%J Journal of Zhejiang University SCIENCE D
%V 7
%N 2
%P 153-166
%@ 1869-1951
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1007/s42242-023-00267-x
TY - JOUR
T1 - Dissolvable temporary barrier: a novel paradigm for flexible hydrogel
patterning in organ-on-a-chip models
A1 - Ding Wang
A1 - Qinyu Li
A1 - Chenyang Zhou
A1 - Zhangjie Li
A1 - Kangyi Lu
A1 - Yijun Liu
A1 - Lian Xuan & Xiaolin Wang
J0 - Journal of Zhejiang University Science D
VL - 7
IS - 2
SP - 153
EP - 166
%@ 1869-1951
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1007/s42242-023-00267-x
Abstract: A combination of hydrogels and microfluidics allows the construction of biomimetic three-dimensional (3D) tissue models
in vitro, which are also known as organ-on-a-chip models. The hydrogel patterning with a well-controlled spatial distribution is
typically achieved by embedding sophisticated microstructures to act as a boundary. However, these physical barriers inevitably
expose cells/tissues to a less physiologically relevant microenvironment than in vivo conditions. Herein, we present a novel
dissolvable temporary barrier (DTB) strategy that allows robust and flexible hydrogel patterning with great freedom of design
and desirable flow stimuli for cellular hydrogels. The key aspect of this approach is the patterning of a water-soluble rigid
barrier as a guiding path for the hydrogel using stencil printing technology, followed by a barrier-free medium perfusion
after the dissolution of the DTB. Single and multiple tissue compartments with different geometries can be established using
either straight or curved DTB structures. The effectiveness of this strategy is further validated by generating a 3D vascular
network through vasculogenesis and angiogenesis using a vascularized microtumor model. As a new proof-of-concept in
vasculature-on-a-chip, DTB enables seamless contact between the hydrogel and the culture medium in closed microdevices,
which is an improved protocol for the fabrication of multiorgan chips. Therefore, we expect it to serve as a promising paradigm
for organ-on-a-chip devices for the development of tumor vascularization and drug evaluation in the future preclinical studies.
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