CLC number:
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2024-01-15
Cited: 0
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Haoyu SUN, Haiyang MA, Li WANG, Yang LIU, Tian HOU, Wenjie TANG, Qing YU, Meiwen AN, Meiling WEN. Biomimetic microchannel network with functional endothelium formed by sacrificial electrospun fibers inside 3D gelatin methacryloyl (GelMA) hydrogel models[J]. Journal of Zhejiang University Science A, 2024, 25(1): 79-96.
@article{title="Biomimetic microchannel network with functional endothelium formed by sacrificial electrospun fibers inside 3D gelatin methacryloyl (GelMA) hydrogel models",
author="Haoyu SUN, Haiyang MA, Li WANG, Yang LIU, Tian HOU, Wenjie TANG, Qing YU, Meiwen AN, Meiling WEN",
journal="Journal of Zhejiang University Science A",
volume="25",
number="1",
pages="79-96",
year="2024",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.A23D0045"
}
%0 Journal Article
%T Biomimetic microchannel network with functional endothelium formed by sacrificial electrospun fibers inside 3D gelatin methacryloyl (GelMA) hydrogel models
%A Haoyu SUN
%A Haiyang MA
%A Li WANG
%A Yang LIU
%A Tian HOU
%A Wenjie TANG
%A Qing YU
%A Meiwen AN
%A Meiling WEN
%J Journal of Zhejiang University SCIENCE A
%V 25
%N 1
%P 79-96
%@ 1673-565X
%D 2024
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A23D0045
TY - JOUR
T1 - Biomimetic microchannel network with functional endothelium formed by sacrificial electrospun fibers inside 3D gelatin methacryloyl (GelMA) hydrogel models
A1 - Haoyu SUN
A1 - Haiyang MA
A1 - Li WANG
A1 - Yang LIU
A1 - Tian HOU
A1 - Wenjie TANG
A1 - Qing YU
A1 - Meiwen AN
A1 - Meiling WEN
J0 - Journal of Zhejiang University Science A
VL - 25
IS - 1
SP - 79
EP - 96
%@ 1673-565X
Y1 - 2024
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.A23D0045
Abstract: Three-dimensional (3D) hydrogel models play a crucial role in tissue engineering for promoting tissue regeneration. A biomimetic microchannel network system in the 3D hydrogel model is necessary for optimal cellular function. This report describes the preparation of a biomimetic hydrogel scaffold with an internal microchannel network, using electrospinning techniques and the sacrificial template method for 3D cell culture. Microchannels and cavities were created within the gelatin methacryloyl (GelMA) hydrogel by sacrificing polyvinyl alcohol (PVA) electrospun fibers (>10 μm), resulting in the creation of microvessel-like channels. Mechanical characterizations, swelling properties, and biodegradation analysis were conducted to investigate the feasibility of a biomimetic microchannel network hydrogel scaffold for 3D cell culture applications. Compared to pure GelMA hydrogel, the hydrogel with microchannels promoted cell proliferation, adhesion, and endothelial tube formation. Moreover, the results confirmed that the biomimetic microchannel network scaffold had a major impact on the distribution and arrangement of human umbilical vein endothelial cells (HUVECs) and can enable the formation of artificial microvasculature by the culture of HUVECs and cell media perfusion.
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