JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.2 P.172-177

http://doi.org/10.1631/jzus.B1900459

Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS

Author(s): Yang Liu, You-Ran Shao, Xiang-Yu Li, Zhi-Ming Wang, Li-Rong Yang, Yu-Zhou Zhang, Mian-Bin Wu, Jian-Ming Yao
Affiliation(s): Biotechnology Center, Institute of Plasma Physics and Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China; more
Corresponding email(s): wumb@zju.edu.cn, jmyao@ipp.ac.cn
Key Words: Metabolism, Lycopene, Nicotine, Blakeslea trispora, GC-MS

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Abstract: blakeslea trispora is a natural source of carotenoids, including β-carotene and lycopene, which have industrial applications. Therefore, classical selective breeding techniques have been applied to generate strains with increased productivity, and microencapsulated β-carotene preparation has been used in food industry (Li et al., 2019). In B. trispora, lycopene is synthesized via the mevalonate pathway (Venkateshwaran et al., 2015). lycopene cyclase, which is one of the key enzymes in this pathway, is a bifunctional enzyme that can catalyze the cyclization of lycopene to produce β-carotene and exhibit phytoene synthase activity (He et al., 2017).

在尼古丁诱导下的三孢布拉霉产番茄红素代谢的分析

概要:在三孢布拉霉发酵番茄红素过程中,阻断剂是必不可少的组分.尼古丁作为目前已知的最有效的番茄红素阻断剂之一,其在工业生产上具有巨大的实用价值.但阻断剂作为菌体的外源毒性生物碱,其对菌体的代谢势必有所改变.本文以尼古丁作为阻断剂,对其在三孢布拉霉发酵过程中的代谢影响做了初步研究.基于气相色谱-质谱(GC-MS)检测技术及小分子代谢组分的主成分分析(PCA),证明尼古丁在三孢布拉霉菌体内严重抑制氨基酸及糖代谢,同时脂肪酸和有机酸代谢也有所减弱,但相对强于糖代谢.因此,在尼古丁作为阻断剂时,为积累色素,碳源的补充应以脂肪酸或甘油为主.
关键词：代谢;番茄红素;尼古丁;三孢布拉霉;气相色谱-质谱

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在尼古丁诱导下的三孢布拉霉产番茄红素代谢的分析

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