Full Text:   <1154>

CLC number: 

On-line Access: 

Received: 1998-12-28

Revision Accepted: 1999-06-18

Crosschecked: 0000-00-00

Cited: 0

Clicked: 3038

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE A 2000 Vol.1 No.2 P.202-206

http://doi.org/10.1631/jzus.2000.0202


TELOMERASE RNA AND TELOMERASE ACTIVITY IN TROPHOBLASTIC TUMORS


Author(s):  ZHENG Wei, SHI Yi-fu, XIE Xing, XU Jian-yun, CHEN Qi, SHEN Ming

Affiliation(s):  Women's Hospital, Medical College, Zhejiang University, Hangzhou, 310006

Corresponding email(s): 

Key Words:  telomerase, RNA, trophoblast, tumor, placenta


Share this article to: More

ZHENG Wei, SHI Yi-fu, XIE Xing, XU Jian-yun, CHEN Qi, SHEN Ming. TELOMERASE RNA AND TELOMERASE ACTIVITY IN TROPHOBLASTIC TUMORS[J]. Journal of Zhejiang University Science A, 2000, 1(2): 202-206.

@article{title="TELOMERASE RNA AND TELOMERASE ACTIVITY IN TROPHOBLASTIC TUMORS",
author="ZHENG Wei, SHI Yi-fu, XIE Xing, XU Jian-yun, CHEN Qi, SHEN Ming",
journal="Journal of Zhejiang University Science A",
volume="1",
number="2",
pages="202-206",
year="2000",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2000.0202"
}

%0 Journal Article
%T TELOMERASE RNA AND TELOMERASE ACTIVITY IN TROPHOBLASTIC TUMORS
%A ZHENG Wei
%A SHI Yi-fu
%A XIE Xing
%A XU Jian-yun
%A CHEN Qi
%A SHEN Ming
%J Journal of Zhejiang University SCIENCE A
%V 1
%N 2
%P 202-206
%@ 1869-1951
%D 2000
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2000.0202

TY - JOUR
T1 - TELOMERASE RNA AND TELOMERASE ACTIVITY IN TROPHOBLASTIC TUMORS
A1 - ZHENG Wei
A1 - SHI Yi-fu
A1 - XIE Xing
A1 - XU Jian-yun
A1 - CHEN Qi
A1 - SHEN Ming
J0 - Journal of Zhejiang University Science A
VL - 1
IS - 2
SP - 202
EP - 206
%@ 1869-1951
Y1 - 2000
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2000.0202


Abstract: 
To gain better understanding of telomerase's possible role in the carcinogenesis of gestational trophoblastic tumors, the authors conducted RT-PCR amplification-based analysis and carried out telomeric repeat amplification to determine the levels of the human telomerase RNA (hTR) and that of telomerase enzymatic activity itself in 43 normal human placental tissues, 35 gestational trophoblastic tumor tissues and three choriocarcinoma cell lines. hTR was expressed in malignant gestational trophoblastic tumor tissues as well as choriocarcinoma cell lines. The results showed that hTR of early placenta villi and a part of hydatidiform mole were positive. But relatively low levels of the hTR could be found in placental tissues. telomerase enzymatic activity was strongly positive in 32 of the 35 (91.4%) gestational trophoblastic tumor tissues and all the three choriocarcinoma cell lines. The enzymatic activity of telomerase itself was detectable at relatively low lelves in 14 of the 21 (66.7%) early placental villi, only three of the 22(13.6%) term placenta were weakly positive. These results suggest that telomerase activity may be correlated with the development of trophoblastic tumors, and so, may be a useful diagnostic marker for detecting the existence of malignant trophoblastic cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Avilion, A.A., Piatyszek, M.A., Gupta, J., et al., 1996. Human telomerase RNA and telomerase activity in immortal cell lines and tumortissues.Cancer Res., 56: 645-650.

[2]Feng, J.Funk, W.D., Wang, S.S., et al., 1995. The RNA component of human telomerase. Science, 269: 1236-1241.

[3]Roberts D.J., Mutter G., 1994. Advances in the molecular biology of gestational trophoblastic disease. J Reproductive Medicine, 39: 201-208.

[4]Song, H.Z., and Wu, P.C., 1988. Studies in Trophoblastic Diseases in China. Pergamon Press, Oxford, U.K.P. 3-27.

[5]Greider, C.W., and Blackburn, E.H., 1985. Identification of a specific telomere terminal transferase activity in tetrahymena extracts. Cell, 43: 405-413.

[6]Haidacher, S., Blaschitz, A., Desoye, G. et al., 1995. Immunohistochemical evidence of P53 protein in human placenta and choriocarcinoma cell line. Molecular Human Reprod, 10: 983-988.

[7]Iversen.P.L., Pirruccello.S.J. Meta J.E. et al., 1995. Antisense oligonucleotides which demonstrate tumor specific action also inhibit telomerase in vitro. Proc Am Assoc Cancer Res. 36:412.

[8]Kanazawa, Y., Ohkawa, K., Ueda, K., et al., 1996. Hammerhead ribozyme-mediated inhibition of telomerase activity in extract of human hepatocellular carcinoma cells. Biochemical and Biophysical Research Communications, 225: 570-576.

[9]Kim, N.W., Piatyszek, M.A., Prowse, K.R., et al., 1994. Specific association of human telomerase activity with immortal cells and cancer. Science, 266: 2011-2015.

[10]Lange, T., 1994. Activation of telomerase in a human tumor. Proc. Natl. Acad.Sci.USA, 91: 2882-2885.

[11]Norton, J.C., and Piatyszek, M.A., 1996. Inhibition of human telomerase activity by peptide nucleic acids. Nature Biotechnol, 14: 615-619.

[12]Park,J.S., Namkoong, S.E., Lee,H.Y., et al., 1992 Expression and amplification of cellular oncogenes in human developing placenta and neoplastic trophoblastic tissue. Asia-Oceania J. Obstet. Gynaecol., 18: 57-64.

[13]Raymond, E., Sun, D., Chen, S. F., et al., 1996 Agents that target telomerase and telomeres. Current Opinion in Biotechnology, 7: 583-591.

[14]Rhyu, M.S., 1995. Telomeres, telomerase, and immmortality. J.Natl. Cancer Inst. 87: 884-894.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE