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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.8 P.656~661

http://doi.org/10.1631/jzus.B0810188


Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells


Author(s):  Yi-feng JIANG, Feng-di ZHAO, Xiao-bo LI, Yan-xia NING, Xiu-ling ZHI, Rui-zhe QIAN, Lian-hua YIN

Affiliation(s):  Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China

Corresponding email(s):   rzqian@shmu.edu.cn, lhyin@shmu.edu.cn

Key Words:  Tryptase, RNA interference, Interleukin-6 (IL-6), Tumor necrosis factor-alpha (TNF-&alpha, ), Endothelial cells


Yi-feng JIANG, Feng-di ZHAO, Xiao-bo LI, Yan-xia NING, Xiu-ling ZHI, Rui-zhe QIAN, Lian-hua YIN. Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells[J]. Journal of Zhejiang University Science B, 2008, 9(8): 656~661.

@article{title="Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells",
author="Yi-feng JIANG, Feng-di ZHAO, Xiao-bo LI, Yan-xia NING, Xiu-ling ZHI, Rui-zhe QIAN, Lian-hua YIN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="8",
pages="656~661",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0810188"
}

%0 Journal Article
%T Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells
%A Yi-feng JIANG
%A Feng-di ZHAO
%A Xiao-bo LI
%A Yan-xia NING
%A Xiu-ling ZHI
%A Rui-zhe QIAN
%A Lian-hua YIN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 8
%P 656~661
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0810188

TY - JOUR
T1 - Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells
A1 - Yi-feng JIANG
A1 - Feng-di ZHAO
A1 - Xiao-bo LI
A1 - Yan-xia NING
A1 - Xiu-ling ZHI
A1 - Rui-zhe QIAN
A1 - Lian-hua YIN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 8
SP - 656
EP - 661
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0810188


Abstract: 
Objective: To explore the effects of down-regulated tryptase expression in mast cells on the synthesis and release of )%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-&alpha;) of vascular endothelial cells. Methods: tryptase-siRNA (small-interfering RNA) vector was constructed to inhibit tryptase expression in P815 cells. The medium of P815 cells treated by the tryptase-siRNA (RNAi-P815 group) or pure vector (P815 group) was collected and used to culture bEnd.3 cells. The messenger RNAs (mRNAs) of IL-6 and TNF-α in bEnd.3 cells and their protein levels in the medium were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: IL-6 and TNF-α mRNAs in bEnd.3 cells cultured in RNAi-P815-conditioned medium decreased significantly compared to those in P815-conditioned medium. Consistently, IL-6 and TNF-α protein levels in the medium of bEnd.3 of RNAi-P815 group were lower than those of P815 group. Conclusion: Reduced tryptase expression significantly inhibited the synthesis and release of IL-6 and TNF-α in vascular endothelial cells. RNA interference targeting tryptase expression may be a new anti-inflammatory strategy for vascular diseases.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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