CLC number: R375+.3
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2018-07-07
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Guang-yong Ye, Ke-yi Wang, Qiao-di Gui, Min Wang. Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2[J]. Journal of Zhejiang University Science B, 2018, 19(8): 654-661.
@article{title="Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2",
author="Guang-yong Ye, Ke-yi Wang, Qiao-di Gui, Min Wang",
journal="Journal of Zhejiang University Science B",
volume="19",
number="8",
pages="654-661",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1800005"
}
%0 Journal Article
%T Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2
%A Guang-yong Ye
%A Ke-yi Wang
%A Qiao-di Gui
%A Min Wang
%J Journal of Zhejiang University SCIENCE B
%V 19
%N 8
%P 654-661
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1800005
TY - JOUR
T1 - Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2
A1 - Guang-yong Ye
A1 - Ke-yi Wang
A1 - Qiao-di Gui
A1 - Min Wang
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 8
SP - 654
EP - 661
%@ 1673-1581
Y1 - 2018
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1800005
Abstract: Objective: The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs). Methods: LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry. Results: LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA). Conclusions: LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.
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