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CLC number: R776.1

On-line Access: 2012-05-04

Received: 2011-08-25

Revision Accepted: 2012-02-28

Crosschecked: 2012-04-16

Cited: 9

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.5 P.402-407

10.1631/jzus.B1100278


Curcumin inhibits proliferation of human lens epithelial cells: a proteomic analysis


Author(s):  Yan-hong Hu, Xiu-rong Huang, Ming-xin Qi, Bu-yuan Hou

Affiliation(s):  Department of Ophthalmology, the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China; more

Corresponding email(s):   12212022@163.com

Key Words:  Curcumin, After-cataract, Posterior capsular opacification (PCO), Proteomics, Lens epithelial cells


Yan-hong Hu, Xiu-rong Huang, Ming-xin Qi, Bu-yuan Hou. Curcumin inhibits proliferation of human lens epithelial cells: a proteomic analysis[J]. Journal of Zhejiang University Science B, 2012, 13(5): 402-407.

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author="Yan-hong Hu, Xiu-rong Huang, Ming-xin Qi, Bu-yuan Hou",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100278"
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%T Curcumin inhibits proliferation of human lens epithelial cells: a proteomic analysis
%A Yan-hong Hu
%A Xiu-rong Huang
%A Ming-xin Qi
%A Bu-yuan Hou
%J Journal of Zhejiang University SCIENCE B
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%P 402-407
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%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100278

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T1 - Curcumin inhibits proliferation of human lens epithelial cells: a proteomic analysis
A1 - Yan-hong Hu
A1 - Xiu-rong Huang
A1 - Ming-xin Qi
A1 - Bu-yuan Hou
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 5
SP - 402
EP - 407
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100278


Abstract: 
Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower compared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8093 and 13767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8093 and 13767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for prevention and treatment of polymerase chain reaction (PCR).

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