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Journal of Zhejiang University SCIENCE A 2002 Vol.3 No.3 P.348~354

http://doi.org/10.1631/jzus.2002.0348


Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L


Author(s):  Sateesh Kumar, XU Meng-kui, CHEN Yu-yin, Ponnuvel, K.M., Datta, R.K.

Affiliation(s):  Department of Sericulture & Apiculture, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   sateesh8@hotmail.com

Key Words:  Restriction fragment length polymorphic (RFLP), Molecular marker, Bombyx mori L, Shell ratio, Bulked segregant analysis (BSA), Near isogenic lines


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Sateesh Kumar, XU Meng-kui, CHEN Yu-yin, Ponnuvel, K.M., Datta, R.K.. Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L[J]. Journal of Zhejiang University Science A, 2002, 3(3): 348~354.

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author="Sateesh Kumar, XU Meng-kui, CHEN Yu-yin, Ponnuvel, K.M., Datta, R.K.",
journal="Journal of Zhejiang University Science A",
volume="3",
number="3",
pages="348~354",
year="2002",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2002.0348"
}

%0 Journal Article
%T Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L
%A Sateesh Kumar
%A XU Meng-kui
%A CHEN Yu-yin
%A Ponnuvel
%A K.M.
%A Datta
%A R.K.
%J Journal of Zhejiang University SCIENCE A
%V 3
%N 3
%P 348~354
%@ 1869-1951
%D 2002
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2002.0348

TY - JOUR
T1 - Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L
A1 - Sateesh Kumar
A1 - XU Meng-kui
A1 - CHEN Yu-yin
A1 - Ponnuvel
A1 - K.M.
A1 - Datta
A1 - R.K.
J0 - Journal of Zhejiang University Science A
VL - 3
IS - 3
SP - 348
EP - 354
%@ 1869-1951
Y1 - 2002
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2002.0348


Abstract: 
Two silkworm strains viz, B20 A (high cocoon shell ratio) and C.Nichi (low cocoon shell ratio) were sib mated for 10 generations to determine the homozygosis. Both bulked segregant analysis(BSA) and near isogenic lines (NIL) studies were done to identify the RFLP markers closely linked to cocoon shell parameters. Three hundred and fifty-two random clones were identified as the low copy number sequence and used for identification of restriction fragment length polymorphic (RFLP) marker linked to cocoon weight and cocoon shell character. In the bulk segregant analysis, DNA from the parents (B20 A, C.Nichi), F1 and F2 progeny of high shell ratio (HSR) and low shell ratio (LSR) were screened for hybridization with the random clones. Polymorphic banding pattern achieved through southern hybridization with different probes indicated the probable correlation of polymorphism with high and low cocoon shell character which are possible landmarks in identifying the putative marker(s) for the cocoon shell character. Out of the 100 probes tried with parents, F1, F2 and their bulks, 10 probes were found to be closely linked to cocoon shell characters.

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