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CLC number: S66

On-line Access: 2010-04-28

Received: 2009-10-08

Revision Accepted: 2010-02-05

Crosschecked: 2010-04-10

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.5 P.332-341

http://doi.org/10.1631/jzus.B0900310


Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses


Author(s):  Umezuruike Linus Opara, Dan Jacobson, Nadiya Abubakar Al-Saady

Affiliation(s):  Department of Horticultural Science, Faculty of AgricSciences, University of Stellenbosch, Stellenbosch 7602, South Africa, Department of Viticulture and Oenology, Institute of Wine Biotechnology, Faculty of AgricSciences, University of Stellenbosch, Stellenbosch 7602, South Africa, Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khod 123, Sultanate of Oman

Corresponding email(s):   opara@sun.ac.za

Key Words:  Musa, Genetic diversity, Amplified fragment length polymorphism (AFLP), Phylogenetics, Principal component analysis (PCA), Hierarchical clustering analysis (HCA), Oman


Umezuruike Linus Opara, Dan Jacobson, Nadiya Abubakar Al-Saady. Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses[J]. Journal of Zhejiang University Science B, 2010, 11(5): 332-341.

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author="Umezuruike Linus Opara, Dan Jacobson, Nadiya Abubakar Al-Saady",
journal="Journal of Zhejiang University Science B",
volume="11",
number="5",
pages="332-341",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900310"
}

%0 Journal Article
%T Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses
%A Umezuruike Linus Opara
%A Dan Jacobson
%A Nadiya Abubakar Al-Saady
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 5
%P 332-341
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900310

TY - JOUR
T1 - Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses
A1 - Umezuruike Linus Opara
A1 - Dan Jacobson
A1 - Nadiya Abubakar Al-Saady
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 5
SP - 332
EP - 341
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900310


Abstract: 
Banana is an important crop grown in oman and there is a dearth of information on its genetic diversity to assist in crop breeding and improvement programs. This study employed amplified fragment length polymorphism (AFLP) to investigate the genetic variation in local banana cultivars from the southern region of oman. Using 12 primer combinations, a total of 1094 bands were scored, of which 1012 were polymorphic. Eighty-two unique markers were identified, which revealed the distinct separation of the seven cultivars. The results obtained show that AFLP can be used to differentiate the banana cultivars. Further classification by phylogenetic, hierarchical clustering and principal component analyses showed significant differences between the clusters found with molecular markers and those clusters created by previous studies using morphological analysis. Based on the analytical results, a consensus dendrogram of the banana cultivars is presented.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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