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CLC number: S643.7

On-line Access: 2013-04-03

Received: 2012-09-09

Revision Accepted: 2013-03-03

Crosschecked: 2013-03-16

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.4 P.279-288


Determination of the genetic diversity of vegetable soybean [Glycine max (L.) Merr.] using EST-SSR markers

Author(s):  Gu-wen Zhang, Sheng-chun Xu, Wei-hua Mao, Qi-zan Hu, Ya-ming Gong

Affiliation(s):  Institute of Vegetable Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; more

Corresponding email(s):   gongym07@126.com

Key Words:  Expressed sequence tag (EST), Simple sequence repeat (SSR), Genetic diversity, Microsatellites, Vegetable soybean

Gu-wen Zhang, Sheng-chun Xu, Wei-hua Mao, Qi-zan Hu, Ya-ming Gong. Determination of the genetic diversity of vegetable soybean [Glycine max (L.) Merr.] using EST-SSR markers[J]. Journal of Zhejiang University Science B, 2013, 14(4): 279-288.

@article{title="Determination of the genetic diversity of vegetable soybean [Glycine max (L.) Merr.] using EST-SSR markers",
author="Gu-wen Zhang, Sheng-chun Xu, Wei-hua Mao, Qi-zan Hu, Ya-ming Gong",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Determination of the genetic diversity of vegetable soybean [Glycine max (L.) Merr.] using EST-SSR markers
%A Gu-wen Zhang
%A Sheng-chun Xu
%A Wei-hua Mao
%A Qi-zan Hu
%A Ya-ming Gong
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 4
%P 279-288
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200243

T1 - Determination of the genetic diversity of vegetable soybean [Glycine max (L.) Merr.] using EST-SSR markers
A1 - Gu-wen Zhang
A1 - Sheng-chun Xu
A1 - Wei-hua Mao
A1 - Qi-zan Hu
A1 - Ya-ming Gong
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 4
SP - 279
EP - 288
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200243

The development of expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating plant genetic diversity. In the present study, 22 polymorphic EST-SSRs from grain soybean were identified and used to assess the genetic diversity in 48 vegetable soybean accessions. Among the 22 EST-SSR loci, tri-nucleotides were the most abundant repeats, accounting for 50.00% of the total motifs. GAA was the most common motif among tri-nucleotide repeats, with a frequency of 18.18%. Polymorphic analysis identified a total of 71 alleles, with an average of 3.23 per locus. The polymorphism information content (PIC) values ranged from 0.144 to 0.630, with a mean of 0.386. Observed heterozygosity (Ho) values varied from 0.0196 to 1.0000, with an average of 0.6092, while the expected heterozygosity (He) values ranged from 0.1502 to 0.6840, with a mean value of 0.4616. Principal coordinate analysis and phylogenetic tree analysis indicated that the accessions could be assigned to different groups based to a large extent on their geographic distribution, and most accessions from China were clustered into the same groups. These results suggest that Chinese vegetable soybean accessions have a narrow genetic base. The results of this study indicate that EST-SSRs from grain soybean have high transferability to vegetable soybean, and that these new markers would be helpful in taxonomy, molecular breeding, and comparative mapping studies of vegetable soybean in the future.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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