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CLC number: Q789

On-line Access: 2013-04-03

Received: 2012-10-12

Revision Accepted: 2013-01-30

Crosschecked: 2013-03-21

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.4 P.289-298

http://doi.org/10.1631/jzus.B1200278


Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability


Author(s):  Zhang-yue Song, Jing-luan Tian, Wei-zhe Fu, Lin Li, Ling-hong Lu, Lian Zhou, Zhi-hui Shan, Gui-xiang Tang, Hui-xia Shou

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   huixia@zju.edu.cn

Key Words:  Soybean, Agrobacterium-mediated transformation, Genotypes, Stable transgenic efficiency


Zhang-yue Song, Jing-luan Tian, Wei-zhe Fu, Lin Li, Ling-hong Lu, Lian Zhou, Zhi-hui Shan, Gui-xiang Tang, Hui-xia Shou. Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability[J]. Journal of Zhejiang University Science B, 2013, 14(4): 289-298.

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author="Zhang-yue Song, Jing-luan Tian, Wei-zhe Fu, Lin Li, Ling-hong Lu, Lian Zhou, Zhi-hui Shan, Gui-xiang Tang, Hui-xia Shou",
journal="Journal of Zhejiang University Science B",
volume="14",
number="4",
pages="289-298",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200278"
}

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%A Zhang-yue Song
%A Jing-luan Tian
%A Wei-zhe Fu
%A Lin Li
%A Ling-hong Lu
%A Lian Zhou
%A Zhi-hui Shan
%A Gui-xiang Tang
%A Hui-xia Shou
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%N 4
%P 289-298
%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200278

TY - JOUR
T1 - Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability
A1 - Zhang-yue Song
A1 - Jing-luan Tian
A1 - Wei-zhe Fu
A1 - Lin Li
A1 - Ling-hong Lu
A1 - Lian Zhou
A1 - Zhi-hui Shan
A1 - Gui-xiang Tang
A1 - Hui-xia Shou
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 4
SP - 289
EP - 298
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200278


Abstract: 
The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation. Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system. In this study, twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection, regeneration capacity, and stable transgenic efficiency. Three genotypes, Yuechun 04-5, Yuechun 03-3, and Tianlong 1, showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system. For the Tianlong 1, the average stable transformation efficiency is 4.59%, higher than that of control genotypes (Jack and Williams 82), which is enough for further genomic research and genetic engineering. While polymerase chain reaction (PCR), LibertyLink strips, and β-glucuronidase (GUS) staining assays were used to detect the insertion and expression of the transgene, leaves painted with 135 mg/L Basta could efficiently identify the transformants.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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