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Bio-Design and Manufacturing  2018 Vol.4 No.4 P.463~468

10.1631/jzus.2003.0463


Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment


Author(s):  XIE Guan-lin, SOAD Algam, SWINGS J., MEW T. W.

Affiliation(s):  Istitute of Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   glxie@zju.edu.cn

Key Words:  Rice seed, Non-pathogenic bacteria, Antagonistic bacteria, Pseudomonas


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XIE Guan-lin, SOAD Algam, SWINGS J., MEW T. W.. Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment[J]. Journal of Zhejiang University Science D, 2018, 4(4): 463~468.

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DOI - 10.1631/jzus.2003.0463


Abstract: 
With the use of a seed washing technique, more than 4000 Gram negative bacteria were isolated by two improved isolation methods from 446 batches of 1 kg rice seed samples obtained from 22 provinces in the Philippines. They were initially characterized on the basis of colony morphology and results of biochemical and pathogenicity tests. Six hundred and fifty-two strains were further identified by Biolog, from which 133 were selected for fatty acid methyl ester (FAME) analysis together with 80 standard reference!strains. Sixteen species or types of Pseudomonas and 17 genera of non-pseudomonads were identified, more than one third of which have not been recorded in rice. The most predominant species observed were P. putida and P. fulva. About 17% of the strains of Pseudomonas and 2% of the non-pseudomonads were antagonistic to one or more fungal or bacterial pathogens of rice. rice seed is an important source of biological control agents.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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[3]Cottyn, B., Regalado, E., Mew, T.W. and Swings, J., 2001. Bacterial population associated with rice seed in the tropical environment. Phytopathology, 91:282-292.

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[7]Stead, D. E., 1989. Grouping of Xanthomonas campestris pathovars of cereals and grasses by fatty acid profiling. OEPP Bull, 19:57-68.

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[9]Xie, G. L. and Mew, T. W., 1998. A leaf inoculation method for detection of Xanthomonas oryzae pv. oryzicola from rice seed. Plant Dis., 82: 1007-1011.

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