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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.8 P.995~1000

http://doi.org/10.1631/jzus.2004.0995


VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension


Author(s):  GONG Fang-qi, LIN You-min, TANG Hong-feng, GU Wei-zhong, WANG Wei, KANG Man-li

Affiliation(s):  Affiliated Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   gongfangqi@zju.edu.cn

Key Words:  Bleomycin, Pulmonary hypertension, VEGF, eNOS, mRNA


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GONG Fang-qi, LIN You-min, TANG Hong-feng, GU Wei-zhong, WANG Wei, KANG Man-li. VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension[J]. Journal of Zhejiang University Science A, 2004, 5(8): 995~1000.

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author="GONG Fang-qi, LIN You-min, TANG Hong-feng, GU Wei-zhong, WANG Wei, KANG Man-li",
journal="Journal of Zhejiang University Science A",
volume="5",
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pages="995~1000",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0995"
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%T VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension
%A GONG Fang-qi
%A LIN You-min
%A TANG Hong-feng
%A GU Wei-zhong
%A WANG Wei
%A KANG Man-li
%J Journal of Zhejiang University SCIENCE A
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%P 995~1000
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%DOI 10.1631/jzus.2004.0995

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T1 - VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension
A1 - GONG Fang-qi
A1 - LIN You-min
A1 - TANG Hong-feng
A1 - GU Wei-zhong
A1 - WANG Wei
A1 - KANG Man-li
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 8
SP - 995
EP - 1000
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.0995


Abstract: 
Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonary arteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbits treated with intratracheal bleomycin (BLM). Methods: Immature rabbits were divided into control and BLM group. Two and four weeks after intratracheal normal saline or BLM injection, the systolic, diastolic and mean pulmonary artery pressure (PASP, PADP, MPAP) were measured by micro-catheter; the pathological changes and the expression of VEGFmRNA and eNOSmRNA of endothelial cells in pulmonary arteries were evaluated by HE and in situ hybridization. Results: Two and four weeks after intratracheal injection of BLM, the PASP, PADP and MPAP increased 53%, 49%, 52% in 2 weeks, and 43%, 89%, 56% in 4 weeks; the wall thickness increased and the cavity in middle and small pulmonary arteries became narrow; the Thickness Index (TI) and Area Index (AI) increased 25%, 14% in 2 weeks, and 22%, 24% in 4 weeks; the level of VEGFmRNA and eNOSmRNA expression decreased 46%, 43% in 2 weeks, and 43%, 51% in 4 weeks. There was no significant difference between 2 weeks and 4 weeks BLM groups. Conclusion: The pulmonary artery pressure was elevated, the thickness of wall increased and the cavity became narrow in middle and small pulmonary arteries, and the level of VEGFmRNA and eNOSmRNA expression in pulmonary arterial endothelial cells decreased in immature rabbits after 2 weeks and 4 weeks of intratracheal 4 U/kg BLM injection.

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