Similar articles

Abstract: Two stable transformed lines containing antisense LeETR1 or LeETR2 sequences and their hybridized line were investigated to determine the effect of LeETR1 and LeETR2 specificity in the ethylene receptor family in tomato (Lycopersicon esculentum Mill.) on ethylene signaling. The transgenic line ale1 containing antisense LeETR1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B1. The transgenic line ale2 containing antisense LeETR2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeETR1 and LeETR2 were close to ale1 in many aspects. These results suggested that LeETR1 probably plays a relatively important role in ethylene signaling of tomato growth and development.

[1] Bleecker, A.B., Kende, H., 2000. Ethylene: a gaseous signal molecule in plants. Annu. Rev. Cell Dev. Biol., 16(1):1-18.

[2] Cancel, J.D., Larsen, P.B., 2002. Loss-of-function mutations in the ethylene receptor ERT1 cause enhanced sensitivity and exaggerated response to ethylene in Arabidopsis. Plant Physiol., 129(4):1557-1567.

[3] Chang, C., Stadler, R., 2001. Ethylene hormone receptor action in Arabidopsis. Bioessays, 23(7):619-627.

[4] Ciardi, J.A., Tieman, D.M., Lund, S.T., Jones, J.B., Stall, R.E., Klee, H.J., 2000. Response to Xanthomonas campestris pv. vesicatoria in tomato involves regulation of ethylene receptor gene expression. Plant Physiol., 123(1):81-92.

[5] Ciardi, J., Tieman, D., Jones, J., Klee, H.J., 2001. Reduced expression of the tomato ethylene receptor LeETR4 enhances the hypersensitive response to Xanthomonas campestris pv. vesicatoria. Mol. Plant Microbe Interactions, 14(4):487-495.

[6] Hajdukiewicz, P., Svab, Z., Maliga, P., 1994. The small, versatile pPZP family of Agrobacterium binary vectors. Plant Mol. Biol., 25(6):989-994.

[7] Hua, J., Meyerowitz, E.M., 1998. Ethylene responses are negatively regulated by a receptor gene family in Arabisopsis thaliana. Cell, 94(2):261-271.

[8] Johnson, P.R., Ecker, J.R., 1998. The ethylene gas signal transduction pathway: a molecular perspective. Annu. Rev. Genet., 32(1):227-254.

[9] Klee, H., Tieman, D., 2002. The tomato ethylene receptor gene family: form and function. Physiol. Plant., 115(3):336-341.

[10] Klimyuk, V.L., Carroll, B.J., Thomas, C.M., Jones, J.D.G., 1993. Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J., 3(3):493-494.

[11] Lashbrook, C.C., Tieman, D.M., Klee, H.J., 1998. Differential regulation of the tomato ETR gene family throughout lant development. Plant J., 15(2):243-252.

[12] Lincoln, C., Turner, J., Estelle, M., 1992. Hormone-resistant mutants of Arabidopsis have an attenuated response to Agrobacterium strains. Plant Physiol., 98(3):979-983.

[13] Tieman, D.M., Klee, H.J., 1999. Differential expression of two novel members of the tomato ethylene-receptor family. Plant Physiol., 120(1):165-172.

[14] Tieman, D.M., Taylor, M.G., Ciardi, J.A., Klee, H.J., 2000. The tomato ethylene receptors NR and LeERT4 are negative regulators of ethylene response and exhibit functional compensation within a multigene family. Proc. Nat. Acad. Sci. USA, 97(10):5663-5668.

[15] Wang, H., Qi, M.Q., Cutler, A.C., 1993. A simple method of preparing plant samples for PCR. Nucleic Acid. Research, 21(17):4153-4154.

[16] Whitelaw, C.A., Lyssenko, N.N., Chen, L., Zhou, D., Mattoo, A.K., Tucker, M.L., 2002. Delayed abscission and shorter internodes correlate with a reduction in the ethylene receptor LeERT1 transcript in transgenic tomato. Plant Physiol., 128(3):978-987.

[17] Wilkinson, J.Q., Lanahan, M.B., Yen, H.C., Giavannoni, J.J., Klee, H.J., 1995. An ethylene-inducible component of signal transduction encoded by never-ripe. Science, 270(5243):1807-1809.

[18] Zheng, T.S., Ying, T.J., Zeng, G.W., He, G.Q., 2001. Expression of ethylene receptor gene LeERT2 in tomato mutant Epi and construction of its antisense expression vector. J. Zhejiang Univ. (Agr. and Life Sci.), 27(2):156-160 (in Chinese).

[19] Zhou, D., Kalaitzis, P., Mattoo, A.K., Tucker, M.L., 1996a. The mRNA for an ERT1 homologue is constitutively expressed vegetative and reproductive tissue. Plant Mol. Biol., 30(6):1331-1338.

[20] Zhou, D., Mattoo, A.K., Tucker, M.L., 1996b. Molecular cloning of a tomato cDNA (accession No. U47279) encoding an ethylene receptor. Plant Physiol., 110(4):1435.