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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.5 P.345~351


First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species

Author(s):  JIANG Li-qin, LI Xu-qing, ZHENG Jing-wu

Affiliation(s):  Institute of Biotechnology, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   jwzheng@zju.edu.cn

Key Words:  Bursaphelenchus rainulfi, Pine wood, Morphology, Morphometrics, ITS-RFLP, Phylogeny

JIANG Li-qin, LI Xu-qing, ZHENG Jing-wu. First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species[J]. Journal of Zhejiang University Science B, 2007, 8(5): 345~351.

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author="JIANG Li-qin, LI Xu-qing, ZHENG Jing-wu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species
%A JIANG Li-qin
%A LI Xu-qing
%A ZHENG Jing-wu
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 5
%P 345~351
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0345

T1 - First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species
A1 - JIANG Li-qin
A1 - LI Xu-qing
A1 - ZHENG Jing-wu
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 5
SP - 345
EP - 351
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0345

Bursaphelenchus rainulfi isolated from dead pine trees in Zhejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit (LSU) rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Ambrogioni, L., Caroppo, S., 2002. II genere Bursaphelenchus in Europa Con Particolare Riferimento al complesso dei nematodi del legno del pino. Nematologia Mediterranea, 30(Suppl.):95-106 (in Italian).

[2] Ambrogioni, L., Irdani, T., Caroppo, S., 2003. Records of Bursaphelenchus species on coniferous wood imported from Asian Russia and China to Italy. Redia, 86:139-146.

[3] Braasch, H., 2001. Bursaphelenchus species in conifers in Europe: distribution and morphological relationships. Bulletin OEPP/EPPO Bulletin, 31(2):127-142.

[4] Braasch, H., 2004. A new Bursaphelenchus species (Nematoda: Parasitaphelenchidae) sharing characters with Ektaphelenchidae from the People’s Republic of China. Zootaxa, 624:1-10.

[5] Braasch, H., Burgermeister, W., 2002. Bursaphelenchus rainulfi sp. n. (Nematoda: Parasitaphelenchidae), first record of the genus Bursaphelenchus Fuchs, 1937 from Malaysia. Nematology, 4(8):971-978.

[6] Braasch, H., Gu, J.F., Burgermeister, W., Zhang, J.C., 2005. Bursaphelenchus doui sp. n. (Nematoda: Parasitaphelenchidae) in packaging wood from Taiwan and South Korea—a new species of the xylophilus group. Russian Journal of Nematology, 13(1):19-27.

[7] Burgermeister, W., Metge, K., Braasch, H., Buchbach, E., 2005. ITS-RFLP patterns for differentiation of 26 Bursaphelenchus species (Nematoda: Parasitaphelenchidae) and observations on their distribution. Russian Journal of Nematology, 13(1):29-42.

[8] Dan, Y., Yang, P.W., Li, J.L., Yu, S.F., 2003. PCR amplification of ribosomal DNA in four species of Bursaphelenchus. Journal of Yunnan Agricultural University, 18(3):309-311 (in Chinese).

[9] de Ley, P., Félix, M.A., Frisse, L.M., Nadler, S.A., Sternberg, P.W., Thomas, W.K., 1999. Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1(6):591-612.

[10] Evans, H.F., Mcnamara, D.G., Braasch, H., Chadoeuf, J., Magnusson, C., 1996. Pest risk analysis (PRA) for the territories of the European Union (as PRA area) on Bursaphelenchus xylophilus and its vectors in the genus Monochamus. OEPP/EPPO Bulletin, 26(2):199-249.

[11] Fang, Y.S., Zhao, J., Zhuo, K., 2002a. Description of Bursaphelenchus dongguanensis sp. n. in China (Nematoda: Aphelenchoididae). Journal of Huazhong Agricultural University, 21(2):109-111 (in Chinese).

[12] Fang, Y.S., Zhuo, K., Zhao, J., 2002b. Description of Bursaphelenchus aberrans n. sp. (Nematoda: Parasitaphelenchidae) isolated from pine wood in Guangdong Province, China. Nematology, 4(7):791-794.

[13] Ferris, V.R., Ferris, J.M., Faghihi, J., 1993. Variations in spacer ribosomal DNA in some cyst forming species of plant parasitic nematodes. Fundamental and Applied Nematology, 16(2):177-184.

[14] He, Y., Subbotin, S., Rubtsova, T.V., Lamberti, F., Brown, D.J.F., Moens, M., 2005. A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida). Nematology, 7(1):111-124.

[15] Hoyer, V.U., Burgermeister, W., Braasch, H., 1998. Identification of Bursaphelenchus species (Nematoda: Aphelenchoididae) on the basis of amplified ribosomal DNA (ITS-RFLP). Nachrichtenblatt des Deutschen Pflanzenschutzdiensites, 50(11):273-277.

[16] Hunt, D.J., 1993. Aphelenchida, Longidoridae and Trichodoridae, Their Systematics and Bionomics. Cambridge University Press, UK, p.106-109.

[17] Iwahori, H., Tsuda, K., Kanzaki, N., Izui, K., Ftai, K., 1998. PCR-RFLP and sequencing analysis of ribosomal DNA of Bursaphelenchus nematodes related to pine wilt disease. Fundamental and Applied Nematology, 21(6):655-666.

[18] Maafi, Z.T., Subbotin, S.A., Moens, M., 2003. Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology, 5(1):99-111.

[19] Madani, M., Vovlas, N., Castillo, P., Subbotin, S.A., Moens, M., 2004. Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean Basin using RFLPs and sequences of ITS-rDNA. Journal of Phytopathology, 152(4):229-234.

[20] Mamiya, Y., Enda, N., 1979. Bursaphelenchus mucronatus n. sp. (Nematoda: Aphelenchoididae) from pine wood and its biology and pathogenicity to pine trees. Nematologica, 25(3):353-361.

[21] Palmisano, A.M., Ambrogioni, L., Tomiczek, C., Brandstetter, M., 2004. Bursaphelenchus sinensis sp. n. and B. thailandae Braasch et Braasch-Bidasak in Packaging wood from China. Nematologia Mediterranea, 32(1):57-65 (in Italian).

[22] Ryss, A., Vieira, P., Mota, M., Kulinich, O., 2005. A synopsis of the genus Bursaphelenchus Fuchs, 1937 (Aphelenchida: Parasitaphelenchidae) with key to species. Nematology, 7(3):393-458.

[23] Subbotin, S.A., Sturhan, D., 2004. Heterodera circeae sp. n. and H. scutellariae sp. n. (Tylenchida: Heteroderidae) from Germany, with notes on the goettingiana group. Nematology, 6(3):343-355.

[24] Vrain, T.C., 1993. Restriction fragment length polymorphism separates species of Xiphinema americanum group. Journal of Nematology, 25(3):361-364.

[25] Wang, H., Pan, C., Chen, Y., 2004. A new record species of genus Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) in Pinus massoniana from China. Journal of Xiamen University, 5(5):727-732 (in Chinese).

[26] Yin, G.L., Fang, Y.S., 1995. The new record of Bursaphelenchus from China (Aphelenchida: Parasitaphelenchidae). Acta Zootaxonomica Sinica, 20(1):5.

[27] Yin, K.C., Fang, Y.S., Tarjan, A.C., 1988. The key to species in the genus Bursaphelenchus with a description of Bursaphelenchus hunanensis sp. n. (Nematoda: Aphelenchoididae) found in pine wood in Hunan Province China. Proceedings of the Helminthological Society of Washington, 55(1):1-11.

[28] Zheng, J., Subbotin, S., He, S., Gu, J., Moens, M., 2003. Molecular characterization of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology, 11(1):17-22.

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