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CLC number: O657.7

On-line Access: 2008-01-16

Received: 2007-11-24

Revision Accepted: 2007-12-24

Crosschecked: 0000-00-00

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.2 P.160~164

http://doi.org/10.1631/jzus.B0720262


Gas chromatographic method for the determination of hexaconazole residues in black tea


Author(s):  Chinnachamy KARTHIKA, Paul James SACHIN

Affiliation(s):  United Planter’ more

Corresponding email(s):   kartheecm@rediffmail.com

Key Words:  Hexaconazole, Residues, Black tea, Florisil, Gas chromatography-nitrogen phosphorus detector (GP-NPD)


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Chinnachamy KARTHIKA, Paul James SACHIN. Gas chromatographic method for the determination of hexaconazole residues in black tea[J]. Journal of Zhejiang University Science B, 2008, 9(2): 160~164.

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Abstract: 
A highly reliable, quantitative and sensitive analytical method for determining the residues of the fungicide, hexaconazole in black tea is described. The proposed method is based on liquid-liquid extraction followed by gas chromatographic determination, using nitrogen phosphorus detector (GC-NPD) for the identification and quantitation of hexaconazole. The most appropriate solvent mixture for extracting hexaconazole residues from black tea was n-hexane:acetone at 1:1 (v/v). The extract was cleaned up by adsorption column chromatography using activated florisil. Performance of the method was assessed by evaluating quality parameters such as recovery value, repeatability, reproducibility, linearity and limits of detection and quantitation. When the method was assessed for repeatability, the percentage of recovery ranged between 86% and 96% while the relative standard deviation was between 0.30% and 2.35%. In studies on reproducibility the recovery ranged from 81% to 85% and relative standard deviation from 1.68% to 5.13%, implying that the method was reliable. A field trial was conducted to verify the application of this method with real samples. Results prove that the validated method was suitable for extracting hexaconazole residues.

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Reference

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