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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.4 P.267-273

10.1631/jzus.B11a0180


Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour


Author(s):  Jun-liang Wang, Qing Xia, An-ping Zhang, Xiao-yan Hu, Chun-mian Lin

Affiliation(s):  International Joint Research Center for Persistent Toxic Substances, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China; more

Corresponding email(s):   azhang@zjut.edu.cn

Key Words:  Organophosphorus pesticides (OPs), α, -Naphthyl acetate esterase (ANAE), Limit of detection (LOD), Enzyme inhibition, Maximum residue levels (MRLs)


Jun-liang Wang, Qing Xia, An-ping Zhang, Xiao-yan Hu, Chun-mian Lin. Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour[J]. Journal of Zhejiang University Science B, 2012, 13(4): 267-273.

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author="Jun-liang Wang, Qing Xia, An-ping Zhang, Xiao-yan Hu, Chun-mian Lin",
journal="Journal of Zhejiang University Science B",
volume="13",
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year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B11a0180"
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%T Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour
%A Jun-liang Wang
%A Qing Xia
%A An-ping Zhang
%A Xiao-yan Hu
%A Chun-mian Lin
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 4
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%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B11a0180

TY - JOUR
T1 - Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour
A1 - Jun-liang Wang
A1 - Qing Xia
A1 - An-ping Zhang
A1 - Xiao-yan Hu
A1 - Chun-mian Lin
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 4
SP - 267
EP - 273
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B11a0180


Abstract: 
The widespread use of organophosphorus pesticides (OPs) poses a great threat to human health and has made the detection of OP residues in food an important task, especially in view of the fact that easy and rapid detection methods are needed. Because OPs have inhibitory effects on the activity of α;-Naphthyl acetate esterase (ANAE) in plants, in this work we evaluated the possibility of detecting OPs in vegetables with ANAE extracted from commercial flour. The limits of detection (LODs) obtained for methamidophos, dichlorvos, phoxim, dimethoate, and malathion in lettuce samples with crude ANAE were 0.17, 0.11, 0.11, 0.96, and 1.70 mg/kg, respectively. Based on the maximum residue limits (MRLs) for OPs in food stipulated by Chinese laws which are 0.05, 0.20, 0.05, 1.00, and 8.00 mg/kg for methamidophos, dichlorvos, phoxim, dimethoate, and malathion, respectively, the esterase inhibition method with crude ANAE had sufficient sensitivity to detect the residues of dichlorvos, dimethoate, and malathion in lettuce, but it could not be used to guarantee the safety of the same samples if methamidophos or phoxim residue was present. The sensitivity of the method was improved by the use of esterase purified by ammonium sulfate salting-out. The LODs obtained for methamidophos and phoxim with purified esterase were lower than the MRLs for these OPs in food. This is a very promising method for the detection of OP residues in vegetables using crude or purified esterase because of its cheapness, sensitivity, and convenience.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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asem@asemelabasy@yahoo.com<asemelabasy@yahoo.com>

2016-01-22 00:41:20

it is very good

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