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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.1 P.7-13


Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice

Author(s):  Yong-fang JIANG, Jing MA, Yan HE, Yong-hong ZHANG, Yun XU, Guo-zhong GONG

Affiliation(s):  Center for Liver Diseases, the Second Xiangya Hospital, Central South University, Changsha 410011, China

Corresponding email(s):   jiangyongfang@hotmail.com

Key Words:  CD40 ligand gene, Hepatocellular carcinoma (HCC), Cationic liposome, Transfection

Yong-fang JIANG, Jing MA, Yan HE, Yong-hong ZHANG, Yun XU, Guo-zhong GONG. Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice[J]. Journal of Zhejiang University Science B, 2009, 10(1): 7-13.

@article{title="Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice",
author="Yong-fang JIANG, Jing MA, Yan HE, Yong-hong ZHANG, Yun XU, Guo-zhong GONG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice
%A Yong-fang JIANG
%A Jing MA
%A Yan HE
%A Yong-hong ZHANG
%A Yun XU
%A Guo-zhong GONG
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 1
%P 7-13
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820178

T1 - Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice
A1 - Yong-fang JIANG
A1 - Jing MA
A1 - Yan HE
A1 - Yong-hong ZHANG
A1 - Yun XU
A1 - Guo-zhong GONG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 1
SP - 7
EP - 13
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820178

Objective: To evaluate the efficacy of cationic liposome-mediated CD40 ligand (CD40L) gene therapy for hepatocellular carcinoma. Methods: 1×106 of parental H22 cells or H22 cells transfected with the expression vector containing murine CD40L cDNA encoding the entire coding region (pcDNA3.1+-mCD40L) were inoculated subcutaneously into the left flanks of syngenic BALB/C mice. The tumor-bearing mice (tumor nodules 10 mm in maximal diameter) received the treatment of the intratumoral injection of pcDNA3.1+-mCD40L/Transfectam, pcDNA3.1+, or phosphate-buffered saline (PBS), or no treatment. The mice were monitored for tumor growth weekly. We examined mCD40L messenger ribonucleic acid (mRNA) expression by reverse transcription polymerase chain reaction (RT-PCR) and the histologic changes in tumors at two weeks after intratumoral injection using immunohistochemical staining of tumor tissues. Results: All mice inoculated with parental H22 cells developed a tumor subcutaneously, and the tumor size increased progressively within three weeks. However, the mice receiving H22-CD40L cells exhibited complete regression of the tumor two weeks after tumor cell inoculation. The tumor-bearing animals with the treatment of pcDNA3.1+ or PBS, or without treatment had progressive tumor growth, while those mice treated with pcDNA3.1+-mCD40L exhibited a significant inhibition of tumor growth. RT-PCR analysis showed that 783-bp fragments corresponding to the mCD40L mRNA were amplified only from pcDNA3.1+-mCD40L treated tumors. The tumor samples from pcDNA3.1+-mCD40L-treated mice showed significant lymphocyte infiltration, apoptotic bodies, and confluent necrosis in the tumor tissues. Conclusion: The tumorigenicity of CD40L-expressing cells was abrogated when the cells were implanted subcutaneously. In vivo gene therapy of established liver tumor nodules in mice by the intratumoral injection of pcDNA3.1+-mCD40L led to significant tumor inhibition. There was mCD40L mRNA expression in the tissues from pcDNA3.1+-mCD40L-treated tumors. The intratumoral injection of pcDNA3.1+-mCD40L induced a strong inflammatory, mainly lymphocytic infiltration of the tumor, and increased the necrotic rate of the neoplastic cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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