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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.5 P.348~354

10.1631/jzus.B0820302


Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate


Author(s):  Li-rong CHEN, Hong-wei ZHOU, Jia-chang CAI, Rong ZHANG, Gong-xiang CHEN

Affiliation(s):  Clinical Laboratory Center, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   chengong218@163.com

Key Words:  Antibiotic resistance, Carbapenem, Enterobacteriaceae, Outer membrane proteins (OMPs)


Li-rong CHEN, Hong-wei ZHOU, Jia-chang CAI, Rong ZHANG, Gong-xiang CHEN. Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate[J]. Journal of Zhejiang University Science B, 2009, 10(5): 348~354.

@article{title="Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate",
author="Li-rong CHEN, Hong-wei ZHOU, Jia-chang CAI, Rong ZHANG, Gong-xiang CHEN",
journal="Journal of Zhejiang University Science B",
volume="10",
number="5",
pages="348~354",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820302"
}

%0 Journal Article
%T Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate
%A Li-rong CHEN
%A Hong-wei ZHOU
%A Jia-chang CAI
%A Rong ZHANG
%A Gong-xiang CHEN
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 5
%P 348~354
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820302

TY - JOUR
T1 - Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate
A1 - Li-rong CHEN
A1 - Hong-wei ZHOU
A1 - Jia-chang CAI
A1 - Rong ZHANG
A1 - Gong-xiang CHEN
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 5
SP - 348
EP - 354
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820302


Abstract: 
Objective: To investigate the mechanism of carbapenem resistance and the occurrence of plasmid-mediated quinolone resistance determinants qnr and aac(6)-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods: An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction (PCR), and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. outer membrane proteins (OMPs) were examined by urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Urea-SDS-PAGE). Results: Minimum inhibitory concentrations (MICs) of imipenem, meropenem, and ertapenem for ZY106 were 2, 4, and 16 μg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-lactamase, and E. coli transconjugant produced IMP-1. Plasmid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrS1-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZY106 lacked an OMP of approximately 38 kDa. Conclusion: It is the first IMP-1-producing enterobacteriaceae in China and the first report of a clinical isolate that harbors both blaIMP and qnrS genes as well. The blaIMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and meropenem susceptibility in E. cloacae.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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