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Received: 2008-08-07

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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.10 P.818~822

http://doi.org/10.1631/jzus.B0860010


Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana


Author(s):  Ai-rong WANG, Wen-wei LIN, Xiao-ting CHEN, Guo-dong LU, Jie ZHOU, Zong-hua WANG

Affiliation(s):  Computer and Information College, Fujian Agriculture and Forestry University, Fuzhou 350002, China; more

Corresponding email(s):   yxxc19204@sina.com, zonghuaw@163.com

Key Words:  Sclerotinia sclerotiorum, Arabidopsis thaliana, Large subunit ribosomal DNA (LSU rDNA), Systematic classification


Ai-rong WANG, Wen-wei LIN, Xiao-ting CHEN, Guo-dong LU, Jie ZHOU, Zong-hua WANG. Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana[J]. Journal of Zhejiang University Science B, 2008, 9(10): 818~822.

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author="Ai-rong WANG, Wen-wei LIN, Xiao-ting CHEN, Guo-dong LU, Jie ZHOU, Zong-hua WANG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="10",
pages="818~822",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0860010"
}

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%T Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana
%A Ai-rong WANG
%A Wen-wei LIN
%A Xiao-ting CHEN
%A Guo-dong LU
%A Jie ZHOU
%A Zong-hua WANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 10
%P 818~822
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0860010

TY - JOUR
T1 - Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana
A1 - Ai-rong WANG
A1 - Wen-wei LIN
A1 - Xiao-ting CHEN
A1 - Guo-dong LU
A1 - Jie ZHOU
A1 - Zong-hua WANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 10
SP - 818
EP - 822
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0860010


Abstract: 
A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fungal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoculation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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