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CLC number: R644

On-line Access: 2010-07-04

Received: 2009-12-07

Revision Accepted: 2010-02-09

Crosschecked: 2010-06-09

Cited: 28

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.7 P.524-530


Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering

Author(s):  Chun-mao Han, Li-ping Zhang, Jin-zhang Sun, Hai-fei Shi, Jie Zhou, Chang-you Gao

Affiliation(s):  Department of Burn, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   hanchunmao1@126.com

Key Words:  Collagen, Chitosan, Fibrin glue, Scaffold, Tissue-engineered skin

Chun-mao Han, Li-ping Zhang, Jin-zhang Sun, Hai-fei Shi, Jie Zhou, Chang-you Gao. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering[J]. Journal of Zhejiang University Science B, 2010, 11(7): 524-530.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering
%A Chun-mao Han
%A Li-ping Zhang
%A Jin-zhang Sun
%A Hai-fei Shi
%A Jie Zhou
%A Chang-you Gao
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 7
%P 524-530
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900400

T1 - Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering
A1 - Chun-mao Han
A1 - Li-ping Zhang
A1 - Jin-zhang Sun
A1 - Hai-fei Shi
A1 - Jie Zhou
A1 - Chang-you Gao
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 7
SP - 524
EP - 530
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0900400

To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Ahmed, Z., Underwood, S., Brown, R.A., 2000. Low concentrations of fibrinogen increase cell migration speed on fibronectin/fibrinogen composite cables. Cell Motility and the Cytoskeleton, 46(1):6-16.

[2]Blomback, B., Bark, N., 2004. Fibrinopeptides and fibrin gel structure. Biophysical Chemistry, 112(2-3):147-151.

[3]Boyce, S.T., Ham, R.G., 1983. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. Journal of Investigative Dermatology, 8l(Suppl. 1):33s-40s.

[4]Karp, J.M., Sarraf, F., Shoichet, M.S., Davies, J.E., 2004. Fibrin-filled scaffolds for bone tissue engineering: an in vivo study. Journal of Biomedical Materials Research Part A, 71(1):162-171.

[5]Kneser, U., Voogd, A., Ohnolz, J., Buettner, O., Stangenberg, L., Zhang, Y.H., Zhang, Y.H., Stark, G.B., Schaefer, D.J., 2005. Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute. Cells Tissues Organs, 179(4):158-169.

[6]Ma, L., Gao, C., Zhou, J., Shen, J., Hu, X., Han, C., 2003a. Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials, 24(26):4833-4841.

[7]Ma, L., Gao, C.Y., Mao, Z.W., Shen, J., Hu, X., Han, C.M., 2003b. Thermal dehydration treatment and glutaraldehyde cross-linking to increase the biostability of collagen-chitosan porous scaffolds used as dermal equivalent. Journal of Biomaterials Science, Polymer Edition, 14(8):861-874.

[8]Mao, J., Zhao, L., de Yao, K., Shang, Q., Yang, G., Cao, Y., 2003. Study of novel chitosan-gelatin artificial skin in vitro. Journal of Biomedical Materials Research Part A, 64(2):301-308.

[9]Sechriest, V.F., Miao, Y.J., Niyibizi, C., Westerhausen-Larson, A., Matthew, H.W., Evans, C.H., Fu, F.H., Suh, J.K., 2000. GAG-augmented polysaccharide hydroglue: a novel biocompatible and biodegradable material to support chondrogenesis. Journal of Biomedical Materials Research, 49(4):534-541.

[10]Sun, J.Z., 2007. Preparation of Collagen-Chitosan/Fibrin Glue Asymmetric Scaffold and Its Application in Construction of Tissue Engineered Skin. MS Thesis, Zhejiang University, Hangzhou, China.

[11]Wang, T.W., Huang, Y.C., Sun, J.S., Lin, F.H., 2003. Organotypic keratinocyte fibroblast cocultures on a bilayer gelatin scaffold as a model of skin equivalent. Biomedical Sciences Instrumentation, 39:523-528.

[12]Wang, T.W., Wu, H.C., Huang, Y.C., Sun, J.S., Lin, F.H., 2006. Biomimetic bilayered gelatin-chondroitin 6 sulfate-hyaluronic acid biopolymer as a scaffold for skin equivalent tissue engineering. Artificial Organs, 30(3):141-149.

[13]Wechselberger, G., Russell, R.C., Neumeister, M.W., Schoeller, T., Piza-Katzer, H., Rainer, C., 2002. Successful transplantation of three tissue-engineered cell types using capsule induction technique and fibrin glue as a delivery vehicle. Plastic and Reconstructive Surgery, 110(1):123-129.

[14]Ye, Q., Zünd, G., Benedikt, P., Jockenhoevel, S., Hoerstrup, S.P., Sakyama, S., Hubbell, J.A., Turina, M., 2000. Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering. European Journal of Cardio-Thoracic Surgery, 17(5):587-591.

[15]Yu, H.Q., Zhou, Y., Hua, P., Tan, W.S., 2002. Calcium-regulated growth and differentiation of the mouse epidermal keratinocytes. Sheng Wu Gong Cheng Xu Bao, 18:626-629 (in Chinese).

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