CLC number: Q93; R51
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2010-05-14
Cited: 16
Clicked: 6253
Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen. Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir[J]. Journal of Zhejiang University Science B, 2010, 11(6): 391-401.
@article{title="Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir",
author="Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen",
journal="Journal of Zhejiang University Science B",
volume="11",
number="6",
pages="391-401",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000056"
}
%0 Journal Article
%T Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
%A Patrick C. Y. Woo
%A Samson S. Y. Wong
%A Jade L. L. Teng
%A Kit-Wah Leung
%A Antonio H. Y. Ngan
%A Dong-qing Zhao
%A Herman Tse
%A Susanna K. P. Lau
%A Kwok-Yung Yuen
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 6
%P 391-401
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000056
TY - JOUR
T1 - Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
A1 - Patrick C. Y. Woo
A1 - Samson S. Y. Wong
A1 - Jade L. L. Teng
A1 - Kit-Wah Leung
A1 - Antonio H. Y. Ngan
A1 - Dong-qing Zhao
A1 - Herman Tse
A1 - Susanna K. P. Lau
A1 - Kwok-Yung Yuen
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 6
SP - 391
EP - 401
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000056
Abstract: A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.
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Open peer comments: Debate/Discuss/Question/Opinion
<1>
Gurt
2010-08-03 20:46:49
The paper "Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species isolated from the blood culture of a patient with metastatic breast carcinoma and oral cavity as its natural reservoir" is a very thorough study