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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.6 P.391-401

http://doi.org/10.1631/jzus.B1000056


Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir


Author(s):  Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen

Affiliation(s):  State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China, Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China, Department of Microbiology, The University of Hong Kong, Hong Kong, China, Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China

Corresponding email(s):   pcywoo@hkucc.hku.hk, skplau@hkucc.hku.hk

Key Words:  Leptotrichia hongkongensis, Bacterium, Blood culture, Anaerobic bacteria, Novel species


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Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen. Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir[J]. Journal of Zhejiang University Science B, 2010, 11(6): 391-401.

@article{title="Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir",
author="Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen",
journal="Journal of Zhejiang University Science B",
volume="11",
number="6",
pages="391-401",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000056"
}

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%T Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
%A Patrick C. Y. Woo
%A Samson S. Y. Wong
%A Jade L. L. Teng
%A Kit-Wah Leung
%A Antonio H. Y. Ngan
%A Dong-qing Zhao
%A Herman Tse
%A Susanna K. P. Lau
%A Kwok-Yung Yuen
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 6
%P 391-401
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000056

TY - JOUR
T1 - Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
A1 - Patrick C. Y. Woo
A1 - Samson S. Y. Wong
A1 - Jade L. L. Teng
A1 - Kit-Wah Leung
A1 - Antonio H. Y. Ngan
A1 - Dong-qing Zhao
A1 - Herman Tse
A1 - Susanna K. P. Lau
A1 - Kwok-Yung Yuen
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 6
SP - 391
EP - 401
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000056


Abstract: 
A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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<1>

Gurt

2010-08-03 20:46:49

The paper "Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species isolated from the blood culture of a patient with metastatic breast carcinoma and oral cavity as its natural reservoir" is a very thorough study

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