Full Text:   <1350>

CLC number: Q943.2

On-line Access: 2011-04-06

Received: 2010-05-05

Revision Accepted: 2010-09-29

Crosschecked: 2011-03-09

Cited: 16

Clicked: 4008

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.4 P.326-334


Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

Author(s):  Abdul Qayyum Rao, Muhammad Irfan, Zafar Saleem, Idrees Ahmad Nasir, Sheikh Riazuddin, Tayyab Husnain

Affiliation(s):  National Centre of Excellence in Molecular Biology, Lahore 53700, Pakistan

Corresponding email(s):   raocemb@yahoo.co.in, tayyab@cemb.edu.pk

Key Words:  Transformation, Gossypium hirsutum, Phytochrome B, Overexpression, Plant growth, Yield

Share this article to: More <<< Previous Article|

Abdul Qayyum Rao, Muhammad Irfan, Zafar Saleem, Idrees Ahmad Nasir, Sheikh Riazuddin, Tayyab Husnain. Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)[J]. Journal of Zhejiang University Science B, 2011, 12(4): 326-334.

@article{title="Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)",
author="Abdul Qayyum Rao, Muhammad Irfan, Zafar Saleem, Idrees Ahmad Nasir, Sheikh Riazuddin, Tayyab Husnain",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)
%A Abdul Qayyum Rao
%A Muhammad Irfan
%A Zafar Saleem
%A Idrees Ahmad Nasir
%A Sheikh Riazuddin
%A Tayyab Husnain
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 4
%P 326-334
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000168

T1 - Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)
A1 - Abdul Qayyum Rao
A1 - Muhammad Irfan
A1 - Zafar Saleem
A1 - Idrees Ahmad Nasir
A1 - Sheikh Riazuddin
A1 - Tayyab Husnain
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 4
SP - 326
EP - 334
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000168

The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium tumefaciens. Integration and expression of PHYB gene in cotton plants were confirmed by molecular evidence. Messenger RNA (mRNA) expression in one of the transgenic lines, QCC11, was much higher than those of control and other transgenic lines. Transgenic cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal conductance. The increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation rate. Data recorded up to two generations, both in the greenhouse and in the field, revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth, with 35% more yield. Moreover, the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism, decrease in apical dominance, and increase in boll size.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Boccalendo, H.E., Polschuk, E.L., Yanovsky, M.J., Sanchez, R.A., Gatz, C., Casal, J.J., 2003. Increased phytochrome B alleviates density effect on tuber yield of field potato crops. Plant Physiol., 133(4):1539-1546.

[2]Chen, M., Chory, J., Fankhauser, C., 2004. Light signal transduction in higher plants. Ann. Rev. Genetics, 38(1):87-117.

[3]Dong, H.Z., Li, W.J., 2007. Variability of endotoxin expression in Bt transgenic cotton. J. Agron. Crop Sci., 193(1):21-29.

[4]Dunwell, J.M., 2000. Transgenic approaches to crop improvement. J. Exp. Biol., 51(Suppl. 1):487-496.

[5]Guo, H., Yang, H., Mockler, T.C., Lin, C., 1998. Regulation of flowering time by Arabidopsis photoreceptors. Science, 279(5355):1360-1363.

[6]Guo, W.Z., Sun, J., Guo, Y.F., Zhang, T.Z., 2001. Investigation of different dosages of inserted Bt genes and their insect-resistance in transgenic Bt cotton. Acta Genet. Sin., 28(7):668-676 (in Chinese).

[7]Hirasawa, T., Tsuchida, M., Ishihara, K., 1992. Relationship between resistance to water transport and exudation rate and the effect of the resistance on the midday depression of stomatal aperture in rice plants. Jpn. J. Crop Sci., 61:145-152.

[8]Hobbs, S.L.A., Warkentin, T.D., Delong, C.M.O., 1993. Transgene copy number can be positively or negatively associated with transgene expression. Plant Mol. Biol., 21(1):17-26.

[9]Husaineid, S.S., Kok, R.A., Schreuder, M.E., Hanumappa, M., Cordonnier-Pratt, M.M., Pratt, L.H., van der Plas, L.H., van der Krol, A.R., 2007. Overexpression of homologus phytochrome genes in tomato exploring the limits in photoreception. J. Exp. Bot., 58(3):615-626.

[10]Jiang, C.Z., Hirasawa, T., Ishihara, K., 1988. Physiological and ecological characteristics of high yielding varieties in rice plants. II. Leaf photosynthetic rates. J. Crop Sci., 57:139-145.

[11]Kendrick, R.E., Kronenberg, G.H.M., 1994. Photomorphogenesis in Plants. Kluwer Academic Publishers, Dordrecht, p.828.

[12]Mathews, S., 2006. Phytochrome-mediated development in land plants: red light sensing evolves to meet the challenges of changing light environments. Mol. Ecol., 15(12):3483-3503.

[13]Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultivars. Plant Physiol., 15:473-497.

[14]Petersen, R.G., 1994. Agricultural Field Experiments: Design and Analysis. Marcel Dekker Inc., NY, USA, p.205-260.

[15]Quail, P.H., 2007. Phytochrome-regulated gene expression. J. Integr. Plant Biol., 49(1):11-20.

[16]Radford, P.J., 1967. Growth analysis formulae-their use and abuse. Crop Sci., 7(3):171-175.

[17]Rao, A.Q., Bakhsh, A., Kiani, S., Shahzad, K., Shahid, A.A., Husnain, T., Riazuddin, S., 2009. The myth of plant transformation. Biotechnol. Adv., 27(6):753-763.

[18]Rao, C.K., 2005. Transgenic Bt Technology: 3. Expression of Transgenes. Available from http://www.monsanto.co.uk/ news/ukshowlib.phtml?uid¼9304

[19]Robson, P.R.H., McCormac, A.C., Irvine, A.S., Smith, H., 1996. Genetic engineering of harvest index in tobacco through overexpression of a phytochrome gene. Nat. Biotechnol., 14(8):995-998.

[20]Said, S.H., Rosan, A.K., Marielle, E.L.S., Mamatha, H., Marie-Michele, C.P., Pratt, L.H., Linus, H.W.V.D.P., Alexander, R.V.D.K., 2007. Overexpression of homologous phytochrome genes in tomato: exploring the limits in photoperception. J. Exp. Bot., 58(3):615-626.

[21]Saiki, R., Chang, C.A., Levenson, C.H., Warren, T.C., Boehm, C.D., Kazazian, H.H.J., Erlich, H.A., 1988. Diagnosis of sickle cell anemia and β-thalassemia with enzymatically amplified DNA and nonradioactive allele-specific oligonucleotide probes. N. Engl. J. Med., 319(9):537-541.

[22]Schittenhelm, S., Hartmann, M.U., Oldenburg, E., 2004. Photosynthesis, carbohydrate metabolism, and yield of phytochrome-B-overexpressing potatoes under different light regimes. Crop Sci., 44(1):131-143.

[23]Shipley, B., 2002. Trade-offs between net assimilation rate and specific leaf area in determining relative growth rate: relationship with daily irradiance. Funct. Ecol., 16(5):682-689.

[24]Somers, D.E., Devlin, P.F., Kay, S.A., 1998. Phytochromes and cryptochromes in the entrainment of the Arabidopsis Circadian clock. Science, 282(5393):1488-1490.

[25]Southern, E.M., 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 98(3):503-517.

[26]Tan, G.Y., Tan, W.K., 1981. Net assimilation rate and relative nitrogen assimilation rate in relation to the dry matter production of alfalfa cultivars. Plant Soil, 59(2):185-192.

[27]Thiele, A., Herold, M., Lenk, I., Quail, P.H., Gatz, C., 1999. Heterologous expression of Arabidopsis phytochrome B in transgenic potato influences photosynthetic performance and tuber. Plant Physiol., 120(1):73-81.

[28]Usami, T., Matsushita, T., Oka, Y., Mochizuki, N., Nagatani, A., 2007. Roles for the N- and C-terminal domains of phytochrome B in interactions between phytochrome B and cryptochrome signaling cascades. Plant Cell Physiol., 48(3):424-433.

[29]Wagner, D., Tepperman, J.M., Quail, P.H., 1991. Overexpression of phytochrome B induces a short hypocotyls phenotype in transgenic Arabidopsis. Cell, 3(12):1275-1288.

[30]Warren, A.L., 2007. Single Cell Gene Expression Analysis by RT PCR. PhD Thesis, California Institute of Technology Pasadena, California, USA, p.133-135.

Open peer comments: Debate/Discuss/Question/Opinion


Imran@SBS<quietsoul800@gmail.com >

2011-04-11 19:35:02

Excellent work

Richa@No address<richapatel925@yahoo.com>

2011-04-09 12:35:46


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE